Biodegradable Polyhydroxyalkanoates as Advanced Antimicrobial Biomaterials: A Review [0.03%]
可降解聚羟基脂肪酸酯类抗菌生物材料的研究进展综述
Shuai He,Longteng Fang,Jing Jing et al.
Shuai He et al.
Polyhydroxyalkanoates (PHA), a class of biodegradable polyesters synthesized by microorganisms, have emerged as a key platform for advanced antimicrobial biomedicine. Distinguished by their exceptional biocompatibility, tunable degradation ...
Tunable and Efficient CO2 Delivery Supports Robust Malic Acid Production by Escherichia coli in a Hollow Fiber Membrane Bioreactor [0.03%]
一种用于支持空纤膜生物反应器中工程菌高效生产的二氧化碳递送系统
Isaiah Woodson,Ryan Duong,Bennett Fisher et al.
Isaiah Woodson et al.
Malic acid, a four-carbon dicarboxylic acid, represents a top value-added chemical from biomass and important building-block molecule for various industrial chemical processes. Currently, however, it is primarily produced from petrochemical...
What Makes an "Ideal" Cell Line for Recombinant Adeno-Associated Virus Production? [0.03%]
重组腺相关病毒生产理想的细胞系应具备哪些条件？
James Conheady,Nicholas Donohue,Alexandra Bogdanovic et al.
James Conheady et al.
Recombinant adeno-associated viruses (rAAVs) have been utilized extensively as the vector-of-choice for in vivo delivery of gene therapy products. Nonetheless, there are several disadvantages associated with current rAAV production systems,...
An Antibiotic-Free Cryptic Plasmid Platform for Modular Pathway Coupling in Probiotic Escherichia Coli Nissle 1917 [0.03%]
用于 probiotic 大肠杆菌 nissle 1917 的模块化途径偶联的无抗生素隐秘质粒平台
Xue Cai,Jie Cui,Lu Xiong et al.
Xue Cai et al.
Escherichia coli Nissle 1917 (EcN) is a well-established probiotic chassis with an excellent safety profile; however, its application as an industrial microbial cell factory remains limited, largely due to genetic instability and antibiotic...
Fouling-Free Inertial Microfluidic Cell Clarification for Lentivirus Manufacturing [0.03%]
用于 lentivirus 制备的无污染惯性微流体细胞澄清装置
Dohyun Park,Alexander Bevacqua,Mingyang Cui et al.
Dohyun Park et al.
Lentiviral vectors (LVVs) are widely used as gene-delivery vehicles in cell and gene therapy, enabling stable integration of transgenes into target cells. However, efficient clarification of LVV harvest remains a major bottleneck in large-s...
Continuous Purification of mRNA Produced by In Vitro Transcription Using High Performance Countercurrent Membrane Purification [0.03%]
基于高性能逆流膜色谱技术的体外转录mRNA连续纯化过程研究
Ziqiao Wang,Amin Javidanbardan,Ali Behboudi et al.
Ziqiao Wang et al.
Messenger ribonucleic acid (mRNA) therapeutics produced by in vitro transcription must be purified to remove residual enzymes and free nucleotides. This study examines the use of high-performance countercurrent membrane purification (HPCMP)...
Magnetoelastic Cell Monitoring System for Evaluating the Effects of Surface Chemistry on the Performance of Mesenchymal Stem Cell Growth [0.03%]
一种磁弹性细胞监测系统用于评估表面化学对间充质干细胞生长性能的影响
William S Skinner,Carolina Rivera-Crespo,Eyerusalem A Gebreyesus et al.
William S Skinner et al.
The recent clinical adoption of cell therapies has increased the need for more efficient, robust, and reproducible manufacturing technologies. Human mesenchymal stromal cells (hMSCs), a major component of the cell and immunotherapeutic mark...
Metabolic Reprogramming and Proteome Reallocation Accompany Loss of Respiratory Oscillations in Yeast Accelerostat [0.03%]
酵母加速仪中呼吸振荡消失伴随代谢重构和蛋白质组重新分配
Gaoya Wang,Yong Chen,Yingping Zhuang et al.
Gaoya Wang et al.
Respiratory oscillations are a hallmark of glucose-limited yeast chemostats, yet how growth rate shapes their emergence and collapse remains unclear. Here, we combined accelerostat cultivation with quantitative metabolomics and proteomics t...
Tetsuo Asakura,Takashi Tanaka,Kenichiro Tatematsu et al.
Tetsuo Asakura et al.
As a biomaterial, silk fibroin (SF) derived from the silkworm Bombyx mori exhibits excellent biocompatibility, controllable biodegradability, minimal inflammatory responses, outstanding mechanical properties, and high processability that en...
A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates [0.03%]
核苷激酶级联通路模块化合成核糖核苷三磷酸
Oliver T Damm,Martin Pfeiffer,Johannes Zöhrer et al.
Oliver T Damm et al.
The increasing demand for affordable nucleoside 5'-triphosphates (NTPs) necessitates efficient and scalable methods of synthesis. Herein, we report standardized one-pot enzymatic cascades for the production of all canonical NTPs (ATP, CTP, ...