Walid M El-Sharoud
Walid M El-Sharoud
Since its inception, synthetic biology has relied on linear genetic modifications to treat living cells as biological counterparts to silicon microchips and computer-aided manufacturing. This paradigm is deeply rooted in the historical fram...
Elucidation and Elimination of Acetyl Phosphate-Driven Catalytic Promiscuity of ArgF/ArgI Enables High-Level L-Arginine Production in Escherichia coli [0.03%]
阐明并消除乙酰磷酸驱动的ArgF / ArgI催化泛化性以在大肠杆菌中高效生产L-精氨酸
Lijuan Wang,Zhi Zhang,Wei Wei et al.
Lijuan Wang et al.
l-Arginine is a critical amino acid widely used in pharmaceutical, food, and feed industries, yet improving its microbial production yield remains challenging due to unresolved metabolic constraints. In this study, we engineered Escherichia...
Synthetic Alternatives to Fetal Bovine Serum: Platforms, Technologies, and Prospects for Industrial Vaccine Production [0.03%]
合成胎牛血清：平台、技术及疫苗工业生产的前景
Sanjay Gottipamula,Venkataramana Neelam Krishnan
Sanjay Gottipamula
Fetal Bovine Serum (FBS) has served as the gold standard supplement in mammalian cell cultures for more than six decades due to its cocktail of growth factors, carrier proteins, hormones, and attachment factors that create an ideal prolifer...
Pectinase-Based Bioprocesses for Circular Bioeconomy and Sustainable Industrial Development [0.03%]
基于果胶酶的生物过程在循环经济和可持续工业发展中的应用
Nisha Sharma,Dimple Tanwar,Usha Grewal et al.
Nisha Sharma et al.
Pectinases play a vital role in the degradation of pectic part of the plant cell wall and are considered in the group of hydrolytic enzymes. In the present scenario, demand of economically feasible and environment friendly techniques and ap...
Expanding Metabolic Online Monitoring Capabilities in 96-Deepwell Microtiter Plates by Combining Optoelectronic O2 and CO2 Sensors [0.03%]
基于光电氧、二氧化碳传感器的96孔深孔板在线代谢监测能力拓展研究
Robert Dinger,David Pfeifer,David Flitsch et al.
Robert Dinger et al.
To increase the information content in early stages of bioprocess development, various technologies have been introduced for monitoring cultivations in microtiter plates (MTPs). Besides spectroscopical measurements, like in the BioLector te...
An Engineered Multicellular Bacterial Network for Simultaneously Answering Multiple Computational Decision Problems [0.03%]
用于同时解决多个计算决策问题的工程化多细胞细菌网络
Biyas Mukherjee,Sangram Bagh
Biyas Mukherjee
Living cell-based computers are in their infancy and answering multiple computational decision problems by a single system remains a key challenge. Here, we demonstrate an artificial neural network type architecture implemented with molecul...
Construction of a Self-Assembled Multi-Enzyme Cascade for Efficient D-Allulose Biosynthesis [0.03%]
高效D-海藻糖生物合成的多酶级联自组装体系构建
Zhe-Ming Wu,Yu Lu,Jin-Chao Zhang et al.
Zhe-Ming Wu et al.
d-Allulose is a valuable low-calorie rare sugar with diverse physiological benefits. Although phosphorylation-dephosphorylation-based multi-enzyme cascades enable efficient d-allulose biosynthesis, the free-enzyme format limits substrate ch...
Inventory Replenishment and Transshipment for Distributed Autologous Cell Therapy Manufacturing Networks [0.03%]
分布式同种自体细胞治疗制造网络的库存补充和调拨
Zhaowei Li,Chelsea C White rd,Kan Wang et al.
Zhaowei Li et al.
Autologous cell therapy (AuCT) manufacturing networks must balance two competing objectives: reducing cost and improving patient access to time-sensitive therapy. This study compares three AuCT manufacturing network designs: a centralized n...
High Cell Density Cultivation of HEK293 Cells Using a 2L Membrane-Stirred Bioreactor [0.03%]
采用2L膜搅拌生物反应器高密度培养HEK293细胞
Lennart Jacobtorweihe,Yasemin van Heuvel,Kathrina Scheiermann et al.
Lennart Jacobtorweihe et al.
The use of perfusion systems for the continuous cultivation of animal cells at high cell density (HCD) for the production of recombinant proteins, viruses, and viral vectors has many advantages. Besides an enhancement of volumetric producti...
Current Applications of Staphylococcal Protein A in Biotechnology and Biomedicine: A Comprehensive Review [0.03%]
金黄色葡萄球菌蛋白A的生物技术及生物医药应用进展综合述评
Shirin Damough,Shadi Damough,Ladan Mafakher et al.
Shirin Damough et al.
Protein A (SpA), a cell wall-associated protein of Staphylococcus aureus, is an immunoglobulin (Ig) binding molecule with high affinity to the Fc moiety of IgG. It can help S. aureus for effective shielding from recognition and opsonization...