IMACulate(DE3), an E. coli Strain for High Purity His-Tagged Protein Purifications
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Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his-tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co-purified with the target p... ...