Kun Zhang,Xin Ni,Peng Cai et al.
Kun Zhang et al.
Efficient xylose utilization is crucial for biomass hydrolysate valorization. However, Komagataella phaffii cannot efficiently utilize xylose. Here, we constructed xylose isomerase (XI)-xylulokinase (XK) pathway, nonoxidative pentose phosph...
Multisite Cre-lox Recombination Enables Regulatory Mechanism Elucidation and Systematic Engineering of Echinocandin B Biosynthesis in Aspergillus nidulans [0.03%]
多位点Cre-Lox重组实现棘白菌素B合成在Aspergillus nidulans中的机制阐明与系统工程改造
Youchu Ma,Yue Zhang,Dongfang Zhang et al.
Youchu Ma et al.
Echinocandin B (ECB), a cyclic lipohexapeptide for synthesizing antifungal drugs, is produced by the nonribosomal peptide synthetase gene cluster in Aspergillus nidulans. However, industrial production remains limited by the inefficiency of...
Bruno S Paulo,Sean B Romanowski,Adjo E Kadjo et al.
Bruno S Paulo et al.
Genome minimization, including the deletion of endogenous gene clusters that encode natural products, is a common strategy to improve the yield of heterologous products. We have been interested in developing Burkholderia sp. FERM BP-3421 as...
Metabolic engineering of Escherichia coli for the high-level production of putrescine [0.03%]
用于高产量生产腐胺的大肠杆菌代谢工程菌株的构建
Yoo-Sung Ko,Je Woong Kim,Alisher Nazarbekov et al.
Yoo-Sung Ko et al.
Putrescine is an important platform chemical used in the manufacture of engineering plastics. To support the development of a sustainable plastics industry, microbial production of putrescine from renewable resources has attracted increasin...
Investigating Overflow Metabolism in Heterotrophic Cultures of the Green Alga Chromochloris zofingiensis [0.03%]
异养培养下极低密度的微藻细胞中溢出代谢的研究——以锌 Finger 藻为例
Michelle Meagher,Dimitrios J Camacho,Sean D Gallaher et al.
Michelle Meagher et al.
Chromochloris zofingiensis is of interest for its ability to perform a reversible trophic switch in the presence of glucose that is characterized by a shutdown of photosynthesis and an accumulation of energy storage metabolites. Previous wo...
Sonali Srivastava,Aakash Chandramouli,Payal Gupta et al.
Sonali Srivastava et al.
Delta lactones are fatty acid-derived aroma compounds that hold tremendous commercial value. As consumer demand for natural flavours continues to rise, the bioproduction of δ-lactones, including δ-decalactone and δ-dodecalactone, is attr...
Precise Biosynthesis of β-1,2-Glucan from Cellulosic Materials by in vitro Metabolic Engineering [0.03%]
利用体外代谢工程精确合成β-1,2-葡萄聚糖
Yunjie Li,Yujiao Lu,Pingping Han et al.
Yunjie Li et al.
The biosynthesis of polysaccharides with precisely defined structures, such as tunable degree of polymerization (DP) and low polydispersity index (PDI), remains a significant challenge in microbial cell factories due to their intricate endo...
Omics analyses decoding mechanisms underlying the self-flocculating phenotype of yeast cells and stress tolerance for robust production [0.03%]
解码酵母细胞自聚集表型及应激耐受的机制以实现稳定生产omics研究
Xue Zhang,Yang Dai,Xin-Qing Zhao et al.
Xue Zhang et al.
A unique self-flocculating yeast strain SPSC01 was developed through protoplast fusion for fuel ethanol production with high product titers. In this study, we conducted comparative multi-omics analyses on SPSC01 to elucidate mechanisms unde...
Metabolic engineering of a plasmid-free, non-auxotrophic Escherichia coli for efficient glycolate production [0.03%]
一种无质粒、非营养缺陷型大肠杆菌的代谢工程改造高效产生甘氧酸
Jinyu Cheng,Xinyi Jiang,Xiaomin Li et al.
Jinyu Cheng et al.
Glycolate, an α-hydroxycarboxylic acid, is widely used in industries such as bioplastics, food, and pharmaceuticals. However, current microbial production methods are limited by the use of plasmids and chemical inducers, hindering their in...
Establishing heterologous betaxanthin pigment biosynthesis in cyanobacteria [0.03%]
在蓝藻中建立异源betaxanthin色素生物合成路径
Sayali S Hanamghar,David A Russo,Silas Busck Mellor et al.
Sayali S Hanamghar et al.
Betalains are water-soluble pigments with two major classes: red-violet betacyanins and yellow-orange betaxanthins. These pigments are increasingly being sought after as natural replacements for synthetic pigments in the food industry. Trad...