Systems metabolic engineering of Escherichia coli for high-level branched-chain fatty acid production via dual precursor pathways [0.03%]
通过双重前体途径系统代谢工程改造大肠杆菌高效合成支链脂肪酸
Mengfan Hu,Cai Feng,Mingjun Li et al.
Mengfan Hu et al.
Branched-chain fatty acids (BCFAs), naturally synthesized by Gram-positive bacteria, are promising feedstocks for the production of advanced biofuels. However, efficient BCFA biosynthesis in Gram-negative bacteria such as Escherichia coli r...
Systematic metabolic engineering of Escherichia coli for high-level production of trans-4-hydroxy-L-proline [0.03%]
系统代谢工程改造大肠杆菌高效生产反式4-羟基-L脯氨酸
Kai Wang,Shu-Ping Tian,Shuo Wan et al.
Kai Wang et al.
As a high-value-added amino acid derivative, trans-4-hydroxy-L-proline (T-4-Hyp) faces key bottlenecks in its microbial production from glucose, including insufficient precursor supply and an imbalance between cell growth and product biosyn...
Discovery, identification of tricyclic sesquiterpene avermitilol synthase, and its heterologous production in plasmid-free Corynebacterium glutamicum [0.03%]
avermitilol大环内酯三环骨架生物合成研究及异源表达
Seong-Eun Kim,Yeo Rang Cho,Hyun Jeong Lee et al.
Seong-Eun Kim et al.
Tricyclic sesquiterpenes are bioactive natural products with broad applications in pharmaceuticals, fragrances, and sustainable aviation biofuels. However, the repertoire of characterized tricyclic sesquiterpene synthases (tSTSs) remains li...
Humanization of N-glycan-dependent protein quality-control system in Kluyveromyces marxianus promotes glycoprotein secretion [0.03%]
乳酸克鲁维酵母N-糖依赖性蛋白质质量检测系统的优化促进了重组蛋白的分泌
Yi Ai,Yuting He,Lunqiang Zhao et al.
Yi Ai et al.
Human N-glycoproteins represent a market worth hundreds of billions of dollars, yet their production in yeast is often limited by misfolding and degradation. However, few strategies have addressed this limitation by targeting differences be...
Corrigendum to "De novo biosynthesis of the polymethoxylated flavones eupatilin and jaceosidin in Saccharomyces cerevisiae" [Metabol. Eng. 97 (2026), 102502] [0.03%]
“从头合成新月花青素和木犀草素在酿酒酵母中的聚合甲氧基黄酮”元数据工程CORRIGENDUM
Jingyan Liu,Tianzhen Sun,Zhongjun Liu et al.
Jingyan Liu et al.
Published Erratum
Metabolic engineering. 2026 Jul 29:102505. DOI:10.1016/j.ymben.2026.102505 2026
Metal Ions Program Flavonolignan Biosynthesis and Enable Photo-Biohybrid Production [0.03%]
金属离子调控黄烷醇黄酮类生物合成并实现光生物杂化产物生成
Jiazeng Yang,Yajing Fan,Howard H Chou
Jiazeng Yang
Flavonolignans are pharmacologically important plant natural products whose production has been limited to extraction from natural sources. Their defining transformation, the oxidative coupling of flavonoid and phenylpropanoid precursors, w...
Engineering a Gluconate Bypass Carbon Entry Architecture for Robust Stationary Phase Biomanufacturing [0.03%]
工程构建稳定的迟缓生长期生物制造葡萄糖酸盐绕过碳输入架构
Utsuki Yano,Payel Sarkar,Michael D Lynch
Utsuki Yano
Two-stage bioprocesses which decouple cell growth from product synthesis are an attractive approach to biomanufacturing. However high levels of production in stationary phase cultures often suffer from a progressive decline in metabolism. W...
Synthetic yeast-bacterium consortium enables co-inducible relayed synthesis of chemicals [0.03%]
合成酵母-菌群联盟 Enables 协同诱导递送合成化学物质
Mingqiang Xu,Jiahui Yu,Xinjie Chen et al.
Mingqiang Xu et al.
Microbial coculture can integrate advantages and overcome the metabolic imbalance of individual species. Programming strain interactions represents a common routine for synthetic microbial communities with distinct species, which causes dif...
Substrate-informed metabolic engineering enables a genome-encoded seven-sugar chassis of Corynebacterium glutamicum for spent sulfite liquor valorization [0.03%]
基质导向代谢工程使谷氨酸棒杆菌成为处理废亚硫酸盐液的七糖底盘菌株
Peng Cao,Michael Kohlstedt,Jens Christmann et al.
Peng Cao et al.
Efficient microbial conversion of heterogeneous carbohydrate mixtures remains a central bottleneck in industrial biotechnology, yet scalable chassis architectures capable of coordinated multi-sugar utilization are limited. Recently, substra...
Metabolic adaptation enabling D-xylose assimilation via the entner-doudoroff pathway in glycolysis-impaired Escherichia coli strains [0.03%]
适应性代谢变化使葡萄糖分解受损的大肠杆菌菌株能够通过Entner-Doudoroff途径同化D-木糖
Camillo Iacometti,Valérie A Delmas,Mélodie Cadillon et al.
Camillo Iacometti et al.
In the model bacterium Escherichia coli, the Embden-Meyerhof-Parnas (EMP) glycolysis pathway is the primary route for carbohydrate metabolism. However, alternative metabolic routes can be activated depending on genetic configurations and av...