Jiazeng Yang,Yajing Fan,Howard H Chou
Jiazeng Yang
Flavonolignans are pharmacologically important plant natural products whose production has been limited to extraction from natural sources. Their defining transformation, the oxidative coupling of flavonoid and phenylpropanoid precursors, w...
Engineering a Gluconate Bypass Carbon Entry Architecture for Robust Stationary Phase Biomanufacturing [0.03%]
工程构建稳定的迟缓生长期生物制造葡萄糖酸盐绕过碳输入架构
Utsuki Yano,Payel Sarkar,Michael D Lynch
Utsuki Yano
Two-stage bioprocesses which decouple cell growth from product synthesis are an attractive approach to biomanufacturing. However high levels of production in stationary phase cultures often suffer from a progressive decline in metabolism. W...
Synthetic yeast-bacterium consortium enables co-inducible relayed synthesis of chemicals [0.03%]
合成酵母-菌群联盟 Enables 协同诱导递送合成化学物质
Mingqiang Xu,Jiahui Yu,Xinjie Chen et al.
Mingqiang Xu et al.
Microbial coculture can integrate advantages and overcome the metabolic imbalance of individual species. Programming strain interactions represents a common routine for synthetic microbial communities with distinct species, which causes dif...
Substrate-informed metabolic engineering enables a genome-encoded seven-sugar chassis of Corynebacterium glutamicum for spent sulfite liquor valorization [0.03%]
基质导向代谢工程使谷氨酸棒杆菌成为处理废亚硫酸盐液的七糖底盘菌株
Peng Cao,Michael Kohlstedt,Jens Christmann et al.
Peng Cao et al.
Efficient microbial conversion of heterogeneous carbohydrate mixtures remains a central bottleneck in industrial biotechnology, yet scalable chassis architectures capable of coordinated multi-sugar utilization are limited. Recently, substra...
Metabolic adaptation enabling D-xylose assimilation via the entner-doudoroff pathway in glycolysis-impaired Escherichia coli strains [0.03%]
适应性代谢变化使葡萄糖分解受损的大肠杆菌菌株能够通过Entner-Doudoroff途径同化D-木糖
Camillo Iacometti,Valérie A Delmas,Mélodie Cadillon et al.
Camillo Iacometti et al.
In the model bacterium Escherichia coli, the Embden-Meyerhof-Parnas (EMP) glycolysis pathway is the primary route for carbohydrate metabolism. However, alternative metabolic routes can be activated depending on genetic configurations and av...
13C metabolic flux analysis and gene expression analysis of perfusion culture reveal metabolic bottlenecks for stable glycosylation profiles of biologics [0.03%]
基于灌流培养的代谢通量分析与基因表达分析揭示生物药稳定糖基化模式的代谢瓶颈
Sarah A Sacco,Tiffany Tang,Sean Engels et al.
Sarah A Sacco et al.
Perfusion Chinese hamster ovary (CHO) cell culture offers potential benefits over fed-batch cultures, including higher productivity and a reduced production footprint. However, maintaining a stable profile of product quality attributes (PQA...
De novo biosynthesis of the polymethoxylated flavones eupatilin and jaceosidin in Saccharomyces cerevisiae [0.03%]
从头合成新补加茋和菊塞定在酿酒酵母中的聚合甲氧基黄酮类化合物
Jingyan Liu,Tianzhen Sun,Zhongjun Liu et al.
Jingyan Liu et al.
Eupatilin (EUP) and jaceosidin (JAC) are bioactive polymethoxylated flavones (PMFs) derived from Artemisia argyi. However, their sustainable production is hindered by the low abundance of this plant and the challenges of regioselective chem...
Metabolic rewiring overcomes physiological constraints in Sphingobium lignivorans SYK-6 for valorization of industrial lignin streams [0.03%]
代谢重构克服了Sphingobium lignivorans SYK-6生理限制以实现工业木质素的高值化转换
Akito Mizuide,Ryo Kato,Masaya Fujita et al.
Akito Mizuide et al.
Lignin-rich industrial streams represent an abundant but underutilized source of renewable aromatic carbon. Efficient biological conversion requires microbial hosts capable of metabolizing chemically diverse lignin-derived aromatic compound...
An Nguyen,Merja Penttilä,Alexander D Frey et al.
An Nguyen et al.
Biopolymer chitin has properties such as biocompatibility, strength, chemical functionality, and biodegradability that are attractive for applications. It could be biotechnologically produced using fungi, but the economical feasibility depe...
Programmable and controllable sexual life cycle for improved evolution in Komegataella phaffii [0.03%]
可编程和可控有性生活周期在毕赤酵母Komegataellaphaffii进化中的应用
Lili Song,Yanyan Wu,Li Hua et al.
Lili Song et al.
The sexual life cycle represents a natural engine for promoting genetic diversity in yeast. However, harnessing it for controllable, rapid evolution in industrial yeast strains remains a big challenge. Here, we developed quantitative mating...