Rewiring methanol metabolism in Komagataella phaffii through implementation and evolution of a heterologous RuMP cycle [0.03%]
通过异源RuMP循环的构建和进化重构毕赤酵母甲醇代谢路径
Miriam Kuzman,Lisa Sanvito,Bernd M Mitic et al.
Miriam Kuzman et al.
Biotechnology holds great potential for sustainable industrial production, with methanol emerging as a promising alternative carbon source due to its availability, cost-effectiveness, and high degree of reduction. Natural methylotrophic mic...
A metabolizable inducer-based carbon-source autoinduction (MICA) system enables low-cost, high-level protein expression and metabolic control in Bacillus subtilis [0.03%]
一种可代谢诱导剂的碳源自诱导(MICA)系统能够使枯草芽孢杆菌进行低成本、高产量的蛋白质表达及代谢调控
Yang Li,Zhendong Li,Yufeng Lin et al.
Yang Li et al.
Bacillus subtilis is widely used for industrial protein production and metabolic synthesis owing to its robustness, safety, and strong secretion capacity. However, high-cost and cytotoxic chemical inducers remain major bottlenecks for large...
Harnessing the yeast nucleus for the efficient production of valuable monoterpenes [0.03%]
利用酵母核来高效生产有价值的单萜类化合物
Jing Wang,Wenbing Cao,Zhaohui Zheng et al.
Jing Wang et al.
Subcellular engineering emerges as a pivotal strategy to amplify terpene production in yeast cell factories. The yeast nucleus, which shares acetyl-CoA pool with the cytosol and maintains nuclear membrane integrity during mitosis, offers un...
Enhanced Methanol Metabolism via Reinforced Cellular Energy and Reducing Power Supply for Sustainable Carbon Conversion [0.03%]
增强细胞能量和还原力供应以实现甲醇的可持续碳转化及其代谢强化
Haiyan Liu,Cheng Zhu,Yun Chen et al.
Haiyan Liu et al.
Methanol, derived from abundant natural gas, biogas methane, coal gasification, biomass conversion, and CO2 reduction processes, presents a promising substrate for bioproduction of chemicals, making its assimilation a key focus of this stud...
Metabolic engineering of Escherichia coli for uridine-5'-diphosphate N-acetylglucosamine production through salvage pathway [0.03%]
通过补救途径工程改造大肠杆菌产生尿苷二磷酸-N-乙酰葡萄糖胺
Han Sun,Liwei Qing,Hao Dong et al.
Han Sun et al.
Uridine-5'-diphosphate N-acetylglucosamine (UDP-GlcNAc) has unique biological activities and is a key precursor for production of glycosaminoglycans and human milk oligosaccharides. Metabolic synthesis is considered as an economic strategy ...
Substrate-informed metabolic engineering of Corynebacterium glutamicum enables balanced glucose-xylose co-utilization for the valorization of lignocellulosic feedstocks [0.03%]
基质导向的谷氨酸棒杆菌代谢工程使葡萄糖和木糖平衡共利用，有利于纤维素原料的高值化利用
David J Mees,Peng Cao,Ann-Kathrin Thönes et al.
David J Mees et al.
Efficient co-utilization of glucose and xylose-the predominant sugars in lignocellulosic and paper-derived hydrolysates-remains a major bottleneck in microbial bioprocessing due to substrate hierarchy and carbon catabolite repression. Here,...
Synthetically primed growth of Pseudomonas putida on 2,4-dinitrotoluene as sole carbon and nitrogen source [0.03%]
以2,4-二硝基甲苯作为唯一氮、碳源的荧光假单胞菌合成增养生长研究
David Rodríguez-Espeso,Irene Del Olmo Lianes,Jim C Spain et al.
David Rodríguez-Espeso et al.
While current genetic tools easily enable transfer of metabolic genes among bacteria, their effective nesting in the recipients depends on biochemical and regulatory compatibilities of the introduced pathway with those of the host. This iss...
Metabolic engineering of Escherichia coli for the production of two-carbon primary amines [0.03%]
大肠杆菌代谢工程生产一、二碳 primary amines
Alisher Nazarbekov,Yoo-Sung Ko,Sang Yup Lee
Alisher Nazarbekov
Primary amines are the industrially important chemicals widely used in the manufacture of dyes, pharmaceuticals, and agrochemicals. In this study, we developed metabolically engineered Escherichia coli strains for the efficient production o...
Reconstruction of arginine deiminase pathway sustains a higher-energy state in mammalian cells [0.03%]
精氨酸脱亚胺酶途径的重建使哺乳动物细胞处于更高的能量状态
Mauro Torres,Matthew Reaney,Kate Meeson et al.
Mauro Torres et al.
ATP is the universal "energy currency" of the cell, and its supply may represent one of several limiting factors influencing the productivity of mammalian cell factories. Here, we present a novel, mitochondria-independent approach to enhanc...
Engineering Halomonas bluephagenesis for high-efficiency biosynthesis of pyruvate [0.03%]
工程改造Halomonas蓝噬菌体以高效生物合成丙酮酸
Kang Wang,Zonghao Zhang,Zhongnan Zhang et al.
Kang Wang et al.
Pyruvate, a C3 platform compound, has significant applications across multiple sectors, including bio-based materials (e.g., polylactic acid), pharmaceutical intermediates (such as L-alanine), and food additives. Biomanufacturing via microb...