Additive-based protein stabilization for stress-prone and unstable proteins [0.03%]
基于添加剂的蛋白质稳定方法在易受压力和不稳定蛋白质中的应用
Tomoto Ura,Takumi Nakamura,Moe Iijima et al.
Tomoto Ura et al.
Protein-stabilizing additives have traditionally been investigated under dilute solution conditions using simple monomeric proteins as model systems. While such studies have clarified fundamental additive-protein interactions, their direct ...
Development and optimization of the hypoglycemic activity of an α-glucosidase inhibitory protein from a UV-induced mutant strain of Streptomyces griseorubens [0.03%]
紫外线诱变链霉菌灰红环菌产生抗糖尿病活性化合物的育种优化研究
Mohammed Alosaimi,Waleed Abdulkhair,Jamal Asseri et al.
Mohammed Alosaimi et al.
This study investigated the isolation, enhancement, and optimisation of an α-glucosidase inhibitory protein with hypoglycaemic activity from Streptomyces species obtained from tomato-cultivated soils rich in actinomycetes. A total of 110 S...
Expression and production of recombinant SARS-CoV-2 RBD protein in E. coli system: comparison of performance, functionality and stability in three strains [0.03%]
大肠杆菌系统中SARS-CoV-2 RBD重组蛋白的表达与生产：三种菌株性能、功能及稳定性的比较分析
Romina Golafshan,Mahmoudreza Aghamali,Mohammad Javad Rasaee
Romina Golafshan
The worldwide COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2). The receptor binding domain (RBD) of SARS-Cov-2 is located on the spike protein, which binds and interacts with ACE2. The RBD serve as a...
DETECTION OF HUMAN PD-1 ON T-CELLS USING A FUSION PROTEIN CONSISTING OF AN ANTI-PD-1 SINGLE CHAIN VARIABLE FRAGMENT LINKED TO SUPERFOLDER GFP [0.03%]
利用含有抗PD-1单链可变片段的超折叠绿色荧光蛋白融合蛋白检测T细胞上的人PD-1
Jong Shin,David Sanford,Alex A Bohm et al.
Jong Shin et al.
Monoclonal antibodies that disrupt the interaction between T-cell derived PD-1 and immunosuppressive ligands, such as PD-L1, have dramatically improved approaches to cancer therapy (reviewed in Tan et al. (2016), Topalian (2015), Pardoll, (...
Novel anti-NS1 human single domain antibody from dengue infected NS1 positive patients [0.03%]
登革热感染阳性患者的新型抗NS1人源单域抗体
Odedara Mitalben Bhikhabhai,Hemant Kumar
Odedara Mitalben Bhikhabhai
Viruses of the flavivirus family significantly affect human health worldwide. Dengue virus (DENV), a member of the flavivirus family, causes pathogenic symptoms like high fever, severe headache and body pain upon infection. In some cases, i...
Expression of Recombinant Preenzyme Blo t 1 Allergen in Escherichia coli and Its IgE Reactivity [0.03%]
大肠杆菌表达的重组预酶型Blo t 1过敏原及其IgE反应性
Chi Linh Thi Nguyen,Uyen Quynh Nguyen,Phuong Mai Vu et al.
Chi Linh Thi Nguyen et al.
Blomia tropicalis is a predominant house dust mite in tropical-subtropical climates, and its allergen Blo t 1 is recognized as a key trigger of allergic responses. This study aimed to express, purify recombinant Blo t 1 from B. tropicalis c...
Expression and functional characterization of recombinant soluble CD40 Ligand in prokaryotic systems [0.03%]
原核系统中重组可溶CD40配体的表达及功能研究
Qi Xu,Xinying He,Jiale Bai et al.
Qi Xu et al.
Objective: To develop an optimized prokaryotic expression system for producing functional soluble CD40 ligand (sCD40L) and characterize its hemostatic activity. ...
The superiority of MabSelect VL, Cytiva's second-generation Protein L resin, over three other affinity resins in purifying a VHH-containing trispecific antibody [0.03%]
一种包含VHH的三特异性抗体在四种亲和介质上的捕获性能比较研究：蛋白L第二代树脂MabSelect?VL优于其他三种亲和介质
Ruomei Lv,Wenjing Qi,Yifeng Li
Ruomei Lv
Affinity chromatography is widely utilized for initial product capture in antibody purification. Besides Protein A resins, other affinity resins that bind different subdomains of an antibody have also been developed. For example, Protein L ...
Recombinant expression of crotoxin and comparison to native crotoxin as immunogens for anti-crotalid antivenoms [0.03%]
重组表达crotoxin并将其与天然crotoxin作为反抗游蛇毒素的免疫原进行比较
Miguel Angel Mejía Sánchez,Samuel Cardoso Arenas,Ricardo Miranda Blancas et al.
Miguel Angel Mejía Sánchez et al.
The crotoxin complex is identified as the primary lethal toxin in various species of rattlesnakes. It comprises two subunits: CtxA, a non-toxic protein, and CtxB, a minimally toxic enzymatic phospholipase A2. These components exert neurotox...
Comparative Analysis of SUMO and His-tag Fusion for Soluble Expression of the SARS-CoV-2 Omicron RBD in E. coli [0.03%]
用于E.coli中表达SARS-CoV-2奥密克戎RBD的SUMO和His标签融合的可溶性表达的比较分析
Sadegh Zargan,Saba Mesdaghinia,Hasan Jalili et al.
Sadegh Zargan et al.
The soluble expression of the SARS-CoV-2 Receptor-Binding Domain (RBD) is fundamental for manufacturing protein-based countermeasures. Although E. coli is a favored host for rapid production, its utility is often constrained by the formatio...