Expression of a recombinant DIVA antigen for differential diagnosis of H7N9 subtype avian influenza virus infected and vaccinated chickens [0.03%]
一种用于鉴别诊断H7N9亚型禽流感病毒感染鸡和疫苗接种鸡的重组DIVA抗原的表达
Wang Yu,Jiao Hu,Xiaoquan Wang et al.
Wang Yu et al.
H7N9 subtype avian influenza virus (AIV) is a great threat for poultry industry in China. Serological assays that differentiate infected and vaccinated animals (DIVA) can facilitate H7N9 virus monitoring in vaccinated poultry. A serological...
Prokaryotic expression, purification of Tldi1 protein and preparation of its polyclonal antibody in Salmonella Typhimurium [0.03%]
大肠杆菌表达、纯化副鼠疫耶尔森氏菌Tldi1蛋白及多克隆抗体的制备
Yunzhu Yang,Chang Liu,Jiaman Chen et al.
Yunzhu Yang et al.
This study aimed to construct a prokaryotic expression vector for the T6SS immunity protein Tldi1 of Salmonella Typhimurium and to prepare its specific polyclonal antibody, thereby facilitating further functional research on Tldi1.The domin...
Soluble Expression, Two-Step Purification and Tagmentation-Compatible Activity Assessment of Hyperactive Tn5 Transposase Using a GB1 Fusion Tag Strategy [0.03%]
利用GB1融合标签策略表达、纯化及活性评估适用于连接酶模块的高活性Tn5转座酶变异体
Yuzhen Xie,Yan Shi,Heyao Zhang et al.
Yuzhen Xie et al.
Background: Hyperactive Tn5 transposase is an essential reagent for tagmentation-based next-generation sequencing (NGS) library construction that includes assays for transposase-accessible chromatin using sequencing and c...
High-level soluble production of firefly luciferase in E. coli via promoter engineering [0.03%]
通过启动子工程在大肠杆菌中高效表达萤火虫荧光素酶
Ling Liu,Jiacheng Zhang,Haorui Xu et al.
Ling Liu et al.
Firefly luciferase (FLuc) is a key enzyme catalyzing bioluminescent reactions. Owing to its strong penetrability of bioluminescence, low background signal, high catalytic efficiency, high sensitivity and specificity for substrates, FLuc is ...
Rapid generation of Drosophila Schneider 2 (S2) cell lines and FACS-based isolation of high-yield soluble or membrane proteins [0.03%]
果蝇Schneider S2细胞系的快速构建及高产量可溶或膜蛋白的FACS分离技术
Stefanie Köhler,Heike Böning,Immo Prinz et al.
Stefanie Köhler et al.
Efficient production of milligram quantities of properly folded, functional eukaryotic proteins remains a significant challenge for structural and biochemical research, especially for membrane proteins and complex glycoproteins. The Drosoph...
Heterologous expression and characterization of multi-resistant manganese superoxide dismutase from Thermus aquaticus in Escherichia coli [0.03%]
来自于Thermus aquaticus的多抗性锰超氧化物歧化酶在大肠杆菌中的异源表达及鉴定
Wanli Guo,Pan Wei,Feng Huang et al.
Wanli Guo et al.
This study describes the heterologous expression and stability profiling of a manganese superoxide dismutase from Thermus aquaticus (TaqMn-SOD). Codon-optimized TaqMn-SOD fused with a C-terminal 6His tag was expressed in Escherichia coli. T...
Assessing different Protein A resins' homodimer separation potentials through processing a two-antibody-containing artificial mixture [0.03%]
通过处理含两种抗体的人工混合物来评估不同蛋白质A树脂的同二聚体分离潜力
Gaoya Yuan,Meng Qu,Yifeng Li
Gaoya Yuan
Homodimers represent the most difficult-to-remove byproducts associated with the production of asymmetric bispecific antibodies (bsAbs). An affinity medium can potentially separate homodimer byproducts if it binds different antibodies with ...
Bioinformatics, expression, and immunogenicity analysis of MPXV A7 protein [0.03%]
MPXV A7蛋白的生物信息学、表达及免疫原性分析
Chenyi Wang,Jiaqi Xiong,Hao Zhou et al.
Chenyi Wang et al.
Since the World Health Organization designated the Monkeypox (mpox) outbreak a Public Health Emergency of International Concern (PHEIC) in 2022, monkeypox virus (MPXV) has spread across all six continents. However, the virus's genomic varia...
Purification and characterization of a new trypsin inhibitor from Senegalia rugata and its toxicity against the larvae of the dengue mosquito Aedes albopictus (Diptera: Culicidae) [0.03%]
Senegalia rugata的一种新型胰蛋白酶抑制剂的纯化及表征及其对登革热蚊子Aedes albopictus幼虫的毒性作用研究
Jemy Jacob,Kannan Vadakkadath Meethal
Jemy Jacob
Plant protease inhibitors are defense molecules found predominantly in the storage tissues of plants and help prevent herbivory. A new trypsin inhibitor was purified to homogeneity from the seeds of Senegalia rugata through sequential steps...
Biochemical characterization of a novel NAD+-dependent alcohol dehydrogenase BaADH9 from Bacillus amyloliquefaciens with C2-selective catalytic sites [0.03%]
来自枯草芽孢杆菌的NAD+依赖性酒精脱氢酶BaADH9的生化特性及C2选择性催化位点分析
Xiaoyang Zhang,Caixia Song,Yujie Chen et al.
Xiaoyang Zhang et al.
Alcohol dehydrogenases (ADHs) are key biocatalysts for the biosynthesis of food flavor additives, but most natural ADHs lack strict substrate selectivity, limiting their industrial application. In this study, a novel NAD+-dependent alcohol ...