Removal of homodimers of bispecific antibody via mixed-mode chromatography with dual pH-salt gradients [0.03%]
Qian Li,Guohong Qin,Hongyang Zhao et al.
Qian Li et al.
Bispecific antibodies (bsAbs) have become a research hotspot in the pharmaceutical field due to their enhanced specificity and reduced side effects. However, their complex structure, especially the unbalanced expression of heavy and light c...
Identification and Characterization of a New Proline-Specific Endopeptidase, PvPSE1, from Paecilomyces variotii [0.03%]
Yuzong Liang,Tong Guo,Xiang Gu et al.
Yuzong Liang et al.
Fungal proline-specific endopeptidases (PSEs) show strong potential in both therapeutic and biotechnological applications, while most known members are acidic proteases from Aspergillus species. Here, we report the identification and charac...
Mutation of the proteolytic cleavage site enhances stability and yield of recombinant hepatitis C virus core protein in bacterial cells [0.03%]
Kyo Izumida,Yumiko Hara,Keisuke Tabata et al.
Kyo Izumida et al.
The Hepatitis C virus (HCV) core protein is crucial for viral assembly and pathogenesis; however, structural studies have been hampered by its instability and historical reliance on denaturing purification methods. Although a recent non-den...
Genetic characterization and mutagenesis of fat-degrading enzymes from Bacillus cereus for enhanced lipid degradation and esterification [0.03%]
芽孢杆菌脂质降解酶的基因组分析及诱变研究增强其脂质降解和酯化能力
Fahad A Al-Dhabaan
Fahad A Al-Dhabaan
Lipases are key biocatalysts for industrial lipid processing. Thirty bacterial isolates were screened for lipolytic activity using qualitative and quantitative assays, identifying Bacillus cereus B18 as the most potent producer (clear zone ...
Structural rationality and enhanced immunoprotective efficacy of endogenously expressed OmpX-IL-9 fusion protein against Salmonella in broilers [0.03%]
结构性合理性和增强的免疫保护效力：鸡中内源性表达OmpX-IL-9融合蛋白抵抗沙门氏菌的效果研究
Kexin Liu,Shuyu Wei,Changquan Liu et al.
Kexin Liu et al.
Salmonella infection severely threatens the broiler industry. OmpX, a promising vaccine candidate, exhibits limited immunogenicity as a single agent, while exogenous IL-9 adjuvants have inherent application deficiencies. This study is the f...
Screening and characterization of EGFR-targeted specific nanobodies [0.03%]
用于筛选和表征EGFR靶向特异性纳米抗体的试验研究
Jing Geng,Long Sha,Yan Liu et al.
Jing Geng et al.
The epidermal growth factor receptor (EGFR), also known as HER1 or ErbB1, is a member of the ErbB receptor family. It plays a critical role in epidermal growth factor (EGF)-mediated cell proliferation and signal transduction pathways. Mutat...
Molecular chaperones and solubility tags: A 44-fold increase in recombinant fibEase yield for therapeutic applications [0.03%]
分子伴侣和溶剂化标签：用于药物研发的重组人弹性蛋白样多肽产量提升44倍的新方法
Zhiqun Che,Suyuan Wei,Renjie Sun et al.
Zhiqun Che et al.
Following codon optimization, the fibE of marine origin achieved successful intracellular expression in Escherichia coli. To enhance production, a combinatorial strategy involving codon optimization, solubility-enhancing tags, molecular cha...
Cloning, Expression, and Biochemical characterization of κ-Carrageenase from Marine Bacterium Cellulophaga sp. P-1 [0.03%]
来自海洋细菌菌株P-1的κ-角叉菜酶的克隆、表达及其生化性质研究
Lin Pan,Yawen Zhang,Zhe Wang et al.
Lin Pan et al.
A novel cold-adapted κ-carrageenase (Car2891) was identified from the marine bacterium Cellulophaga sp. P-1 isolated from Chondrus. The enzyme was recombinantly expressed in E. coli BL21(DE3) and biochemically characterized. Car2891 exhibi...
Heterologous expression of fungal glycoside hydrolase family 115 α-glucuronidases [0.03%]
来源于真菌的几丁质酶家族115α-葡萄糖醛酸酶的异源表达
Michael J Bowman,Nancy N Nichols,Ronald E Hector
Michael J Bowman
Deconstruction of plant xylan is challenging due to the structural complexity arising from side groups on the polymer. One of the side groups, glucuronic acid, can be further modified with a methyl group at the 4-hydroxy position, as well a...
Preparation and evaluation of Escherichia coli-derived glycoprotein E for development of a subunit vaccine against varicella-zoster virus [0.03%]
利用大肠杆菌表达的水痘带状疱疹病毒糖蛋白E重组抗原研究其免疫反应及保护效果
Luyao Zhang,Yao Zhang,Huan Meng et al.
Luyao Zhang et al.
Glycoprotein E (gE) of varicella-zoster virus (VZV) is a validated target for subunit vaccine development. However, gE produced in Chinese hamster ovary (CHO) cells is associated with high manufacturing costs and glycosylation-related heter...