Corrigendum to "Purification and characterization of full-length monomeric TEC family kinase, ITK" [Protein Expr. Purif. 229 (2025) 106682] [0.03%]
“全长度单体TEC家族激酶ITK的纯化与表征”一文的勘误修正
Udumbara M Rathnayake,Junya Wada,Vanessa E Wall et al.
Udumbara M Rathnayake et al.
Published Erratum
Protein expression and purification. 2026 Aug 10:106985. DOI:10.1016/j.pep.2026.106985 2026
Human TBC1 domain-containing kinase is a class I multidomain pseudokinase [0.03%]
人TBC1结构域含有的蛋白激酶是一类多结构域假蛋白激酶
Shiwangi Maurya,Lilly E Cheek,Anthony T Iavarone et al.
Shiwangi Maurya et al.
TBCK-related encephalopathy (TBCKE) is a neurodevelopmental disorder associated with biallelic mutations in TBCK. Despite the increasing number of reported cases worldwide, the biochemical and biophysical properties of TBCK remain unclear, ...
Simultaneous solubility-enhancing domain removal and target purification using the Npu split intein [0.03%]
利用Npu半intein同时进行溶剂化增强域去除和目标纯化
Sophia Mayone,Shanthi Napier,Roman Adomanis et al.
Sophia Mayone et al.
While protein A has become a reliable platform for purifying antibodies, an analogous universal purification method for native non-antibody therapeutics has yet to be widely implemented. Downstream processing of recombinant proteins that re...
Optimization of the Recombinant Overexpression and Purification of Human Nedd4-1 in Escherichia coli [0.03%]
大肠杆菌中人类Nedd4-1重组过表达及纯化条件的优化
Hunter S DeHaan,Abigail R Spear,Dewey G McCafferty
Hunter S DeHaan
The E3 ubiquitin ligase Nedd4-1 is a structurally complex, multidomain enzyme that plays a crucial role in maintaining proteostasis and regulating the cellular stress response. Nedd4-1's complex regulatory mechanism involves both intermolec...
Combining Gene Amplification with SEAP-reporter to Achieve High Expression of Recombinant Omicron XBB.1.5 spike protein [0.03%]
结合基因扩增与SEAP报告基因实现奥密克戎XBB.1.5 Spike蛋白的高效表达
Zhongcheng Zou,Peter D Sun
Zhongcheng Zou
Most stable mammalian recombinant protein expressions use labor-intensive ELISA with target specific antibodies for screening of high-expression clones. Some alternative antibody-free reporter systems, such as the ones using intracellular g...
Expression and immunoassay assessment of immunomodulatory PanDR and SARS-CoV-2 receptor-binding domain antigen in bacteria host cell [0.03%]
细菌宿主细胞中免疫调节蛋白PanDR和SARS-CoV-2受体结合区抗原的表达及免疫测定评估
Rukayat Olajumoke Onilegbale,Gbemisola Morounke Saibu,Habeeb Adebodun Bankole et al.
Rukayat Olajumoke Onilegbale et al.
The gap in technical capacity and inadequate infrastructure for biomanufacturing limits progress in vaccine development and biomanufacturing in low- and middle-income countries (LMICs). Peptide subunit antigens offer a promising solution to...
Recombinant expression of Zika virus NS1 protein in Escherichia coli: effects of fusion strategy and culture conditions [0.03%]
寨卡病毒NS1蛋白在大肠杆菌中的重组表达：融合策略和培养条件的影响
Aliana Saenz de la Torre Leal,Thalía Morán Ávila,Mayra Muné et al.
Aliana Saenz de la Torre Leal et al.
Zika virus (ZIKV) nonstructural protein 1 (NS1) represents a valuable diagnostic target due to its early secretion during infection and reduced cross-reactivity with other flaviviruses. Optimization of NS1 expression conditions is essential...
Construction and evaluation of an inside-out lysis system based on T4 lysozyme [0.03%]
基于T4溶菌酶的“inside-out”裂解系统构建及评价
Wenfeng Ni,Mengya Qin,Xiaoge Yang et al.
Wenfeng Ni et al.
Lysozyme can effectively lyse Escherichia coli (E. coli), providing a method for the release of intracellular substances. In this study, an inside-out lysis system based on T4 lysozyme was constructed. Three secretion signal peptides (PelB,...
Mapping the interactome of recombinant human protein KIAA1143 [0.03%]
重组人蛋白KIAA1143的相互作用图谱_MAPPING THE INTERACTOME OF RECOMBINANT HUMAN PROTEIN KIAA1143
Runzhi Wang,Xiaosong Feng,Yajun Xie
Runzhi Wang
KIAA1143 is located within the 3p21.3 chromosomal region, which is frequently deleted in human cancers and enriched for tumor suppressor genes, and encodes an evolutionarily conserved protein; however, its biological functions and relevance...
Recombinant expression and improved refolding of the SARS-CoV-2 spike receptor-binding domain in Escherichia coli [0.03%]
在大肠杆菌中表达及改进SARS-CoV-2刺突蛋白受体结合域的重折叠属性
Daniela Roa-Velázquez,Jessica Georgina Filisola-Villaseñor,Dan Israel Zavala-Vargas et al.
Daniela Roa-Velázquez et al.
The receptor-binding domain (RBD) of SARS-CoV-2 spike glycoprotein is central to serological diagnostics and vaccine development, but production in eukaryotic expression systems is costly and technically demanding. Here, we describe an Esch...