Recombinant expression and purification of TANB77-derived pilin-like proteins 935 and 938 [0.03%]
TANB77衍生的类菌毛蛋白935和938的重组表达及纯化
Tham Truong Phuong Tran,Linh Thanh Nguyen,Byeong-Gu Han et al.
Tham Truong Phuong Tran et al.
Increasing evidence highlights the gut microbiota as a source of diagnostic biomarkers and a key modulator of anti-tumor immunity and cancer immunotherapy responses. Pilin-like proteins from the uncultured TANB77 clade have shown potential ...
Expression, purification, and reconstitution of small-conductance mechanosensitive channel into lipid bilayer: ready for solid-state NMR study [0.03%]
机控小导通量机械敏感性通道在脂质双层中的表达、提纯及重构：固态核磁共振研究的准备工作
Shenghua Meng,Zixiang Gao,Yuan Lin et al.
Shenghua Meng et al.
Solid-state NMR (ssNMR) provides unique advantages for resolving the structure and interactions of membrane proteins within native-mimicking lipid membranes. However, the challenge is preparing milligrams of stable, homogeneous proteoliposo...
Removal of polysorbates degradation-related impurities by depth filter screening: A case study [0.03%]
基于深度滤器筛选去除聚山梨酯降解相关杂质的研究：一例研究
Chunxiao Xuan,Yangguang Xu,Xinang Cui et al.
Chunxiao Xuan et al.
Depth filtration (DF) is widely utilized as a clarification step in pharmaceutical manufacturing, concurrently removing process-related impurities such as host cell proteins (HCPs) and DNA. This study demonstrates DF's capability in elimina...
Purification and characterization of the peroxiredoxin 5-thioredoxin 2 complex: unraveling the redox regeneration of peroxiredoxin 5 by thioredoxin 2 within the peroxiredoxin catalytic cycle [0.03%]
过氧化物酶体胱氨酸5-硫氧还蛋白2复合物的纯化与特性分析：在过氧化物酶体催化循环中揭示5-硫氧还蛋白2对过氧化物酶体5的氧化还原再生作用
Xiangwei Yang,Yifan Yang,Sizhe Gong et al.
Xiangwei Yang et al.
Peroxiredoxin (Prx) is a critical cysteine-based peroxidase that detoxifies reactive oxygen species to maintain cellular peroxide homeostasis and prevent oxidative stress. Peroxiredoxin 5 (Prx5 or PRDX5) is the unique atypical 2-cysteine Pr...
Hyper-Expression of Functional Human Basic Fibroblast Growth Factor 2 in Escherichia coli [0.03%]
大肠杆菌中人脑源性神经营养因子的高效表达及复性诱导
Tamilvendan Manavalan,Jiawu Bi,Khongsay Naulchan et al.
Tamilvendan Manavalan et al.
The human basic fibroblast growth factor 2 (hbFGF2) is a highly conserved protein that is crucial for cell proliferation, differentiation, and migration. Commercial hbFGF2 is expensive and contributes significantly to the cost of cultivated...
Fine-tuning of leaky expression delivers soluble and functional recombinant alphainterferon in the cytoplasm of Escherichia coli [0.03%]
精细调控漏噬表达在大肠杆菌胞液中获得可溶而有生物活性的重组人干扰素α
Rodrigo Martins Bretas,Marcus Vinícius Alves da Silva,Sophie Yvette Leclercq et al.
Rodrigo Martins Bretas et al.
Alphainterferons are cytokines that became famous in the treatment of certain infections and tumors due to their antiviral, antiproliferative, and immunomodulatory activities. While the production of these small proteins in Escherichia coli...
Robust aggregate separation by Sartobind Rapid A Protein A membrane [0.03%]
Sartobind Rapid A Protein A膜的耐受性研究
Gaoya Yuan,Meng Qu,Yifeng Li
Gaoya Yuan
Protein A resins are the most widely used affinity media for product capture in antibody and Fc-fusion protein purification. However, they generally lack the capability to separate monomers from co-binding aggregates. Recently, Protein A me...
Deciphering the inconsistent performance of Protein A membrane on host cell protein clearance [0.03%]
解读蛋白质A膜清除宿主细胞蛋白性能不一致的机理
Yifan Yang,Xudong Zhang,Yifeng Li
Yifan Yang
In downstream processing of antibodies and Fc-fusion proteins, Protein A affinity chromatography plays an important role in host cell protein (HCP) reduction. Recently, Protein A membrane has emerged as a promising alternative to resin-base...
Bioinformatics analysis and preparation of polyclonal antibodies against the extracellular region of the Langya Virus attachment protein [0.03%]
-langya病毒吸附蛋白胞外区 bioinformatics分析及多克隆抗体的制备
Shuxing Qiu,Xing Yin,Jinjiao He et al.
Shuxing Qiu et al.
The Langya virus attachment glycoprotein (LayV-G) is a major surface glycoprotein critical for host cell entry, membrane fusion, and the induction of neutralizing antibody production. A highly specific, reliable, and cost-effective anti-Lay...
Scalable Production and Characterization of Recombinant Pneumococcal Histidine Triad Protein D in Escherichia coli for Vaccine Development [0.03%]
在大肠杆菌中用于疫苗开发的重组肺炎链球菌组氨酸三联体蛋白D的可放大生产和表征
Prashant Jadhav,Hitesh Malviya,Jyoti Gokhale
Prashant Jadhav
Streptococcus pneumoniae causes 1.5 million deaths annually, primarily in low-income countries. Pneumococcal histidine triad protein D (PhtD), a conserved 92-110 kDa surface protein, is a promising serotype-independent vaccine candidate due...