Expression, purification, and reconstitution of small-conductance mechanosensitive channel into lipid bilayer: ready for solid-state NMR study
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Solid-state NMR (ssNMR) provides unique advantages for resolving the structure and interactions of membrane proteins within native-mimicking lipid membranes. However, the challenge is preparing milligrams of stable, homogeneous proteoliposomes samples to achie... ...