Expression and purification of the ectodomain of erythropoietin receptor fused to mCitrine or mTFP1 fluorescent protein in Escherichia coli [0.03%]
融合mCitrine或mTFP1荧光蛋白的红细胞生成素受体胞外区在大肠杆菌中的表达与纯化
Eunho Kang,Janbolat Ashim,Sangho Ji et al.
Eunho Kang et al.
The erythropoietin receptor (EPOR) is a single-pass transmembrane protein that homo-dimerizes upon binding with its renal ligand erythropoietin (EPO) to trigger downstream signaling. Its extracellular ectodomain mediates ligand binding. The...
Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 [0.03%]
大规模细菌生产及Hsc70稳定磷酸化的研究进展
Nicholas Kley,Michael Hecht-Bucher,Aymelt Itzen
Nicholas Kley
Phosphorylation of the constitutively expressed Hsp70 chaperone Hsc70 at threonine 495 modulates its cellular functions. While the functional consequences of Hsc70 phosphorylation are increasingly understood, analyzing its structural effect...
J Anakha,Yenisetti Rajendra Prasad,Sanjay Chakka et al.
J Anakha et al.
Background and objectives: Angiogenesis plays a central role in the progression of numerous pathological conditions, making its modulation an important therapeutic strategy. Endostatin, a naturally occurring endogenous in...
Persistence and Fab-Specific Interaction of Acyl-Protein Thioestere-1 with Monoclonal Antibodies During Downstream Processing [0.03%]
下游加工过程中酰基蛋白硫酯酶1与单克隆抗体的持久性和fab特异性相互作用
Ernest Šprager,Aleksander Krajnc,Mojca Lunder et al.
Ernest Šprager et al.
Difficult-to-remove host cell proteins (HCPs) can compromise monoclonal antibody (mAb) product quality and stability. This study investigated the persistence of acyl-protein thioesterase-1, a polysorbate-degrading HCP, during downstream pro...
Biophysical characterization of heterologously expressed GrpE and bioinformatic analysis of its native sequence from Neisseria gonorrhoeae [0.03%]
外源性表达的GrpE生物物理表征及其天然序列的生物信息学分析
Jianrong Chang,Jiatong Zhao,Lanlan Mei et al.
Jianrong Chang et al.
The co-chaperone GrpE is a bacterial nucleotide exchange factor essential for DnaK chaperone function and plays a critical role in bacterial pathogenicity. In this study, a putative GrpE from the human pathogen Neisseria gonorrhoeae (NgGrpE...
Expression and purification of recombinant Lopap from Lonomia obliqua (Lepidoptera: Saturniidae) in Spodoptera frugiperda larvae using the baculovirus system [0.03%]
家蚕杆状病毒表达系统在阿根廷灯蛾(Spodoptera frugiperda)中重组表达和纯化斜纹夜盗蛾(Lonomia obliqua)的抗凝血蛋白Lopap
Joaquín Manuel Birenbaum,Juan Félix Onofrio Orlowski,Joaquín Poodts et al.
Joaquín Manuel Birenbaum et al.
A process using Spodoptera frugiperda larvae as biological factories for the expression and one-step purification of the Lonomia obliqua prothrombin activator protein (Lopap) is described. Two recombinant Lopap (Lp) variants were designed, ...
Overcoming hydrophobicity-driven aggregation: An integrated expression strategy enables high-yield soluble production of recalcitrant starch synthases in Escherichia coli [0.03%]
克服疏水性导致的聚集作用：一体化表达策略可在大肠杆菌中实现难以对付的淀粉合成酶的高产量可溶性产生
Yujie Zhu,Yifei Zhao,Xinqi Wang et al.
Yujie Zhu et al.
Diverse enzyme resources are essential for advancing cell-free chemoenzymatic starch synthesis from CO2. However, heterologous expression of such enzymes in Escherichia coli often leads to protein misfolding and inclusion body formation, re...
Construction of a novel B7H3 VHH-Fc antibody with potent antitumor activity and NK/T cell activation [0.03%]
一种具有抗肿瘤活性和激活NK/T细胞的新B7H3 VHH-Fc抗体的构建
Ailing Pan,Caiyi Yuan,Yaru Wang et al.
Ailing Pan et al.
Background: B7-H3 is a tumor-specific antigen capable of regulating both NK and T cell activities. However, current B7-H3 antibodies in clinical or preclinical development lack immunoregulatory functions. ...
Comparison of the Efficacy of Cell Wall Disruption Methods for Saccharomyces cerevisiae INVSc1 cells Expressing Toxoplasma gondii Recombinant ROP6 Protein [0.03%]
用于破坏酿酒酵母表达弓形虫ROP6重组蛋白细胞壁的方法的比较研究
Tuğba Karakavuk,Muhammet Karakavuk,Ceren Gül et al.
Tuğba Karakavuk et al.
Toxoplasma gondii infects both humans and animals, causing severe clinical manifestations. Due to the lack of a safe vaccine, the development of a safe and effective vaccine against toxoplasmosis remains a significant need. Saccharomyces ce...
Refolding-free, Tag-free Production of functional platelet-derived growth factor-BB at Gram-Per-Liter Scale in Escherichia coli [0.03%]
无变复性、无标签人血小板衍生生长因子BB在大肠杆菌中的克隆表达及克氏抽提法高纯度制备工艺开发
Young Su Kim,Hwabong Jeong,Hyejin Lee et al.
Young Su Kim et al.
Platelet-derived growth factor-BB (PDGF-BB) is a homodimeric growth factor essential for angiogenesis, wound healing, and tissue regeneration with well-established therapeutic potential. However, scalable microbial production of bioactive P...