Purification of Antifreeze Proteins from Tussah Silkworm (Antheraea pernyi) via Polyethylene Glycol Precipitation Coupled with Aqueous Two-Phase Extraction [0.03%]
聚乙二醇沉淀与水相萃取联用纯化柞蚕抗冻蛋白
Xinhui Liu,Yiqun Yin,Xin Xu et al.
Xinhui Liu et al.
Antifreeze proteins (AFPs) represent specialized proteins capable of inhibiting ice crystal growth and modifying ice crystal morphology. They hold significant potential in biopharmaceuticals, food storage, and cryopreservation. As a charact...
Bench-top NMR of water signals: A non-destructive tool for biomacromolecule characterization [0.03%]
台式NMR水信号技术：生物大分子表征的无损工具
Tomoto Ura,Taiji Oyama,Chiaki Nishimura
Tomoto Ura
Bench-top NMR spectroscopy has emerged as an accessible, non-destructive tool for analyzing biomacromolecules in solution. This review focuses on how water proton NMR (wNMR) provides sensitive readouts of protein states in solution. We high...
The advantage of high pH eluting Protein A resins over their regular counterparts in aggregate and host cell protein clearance [0.03%]
高pH洗脱的蛋白A树脂在聚集物和宿主细胞蛋白质清除方面的优势
Ziyang Li,Lixia Hu,Yifeng Li
Ziyang Li
Protein A affinity chromatography is the most well-established method and gold standard for antibody purification. Typically, low pH (i.e., 3.0-3.5) is required for elution of bound antibodies from a Protein A column. However, for acid-sens...
Strategic truncation improves recombinant expression of buffalo Fibulin-5 (FBLN5) in Escherichia coli [0.03%]
截短表达改善了重组 buffalo 胶原结合蛋白5在大肠杆菌中的表达
Deviyani Mahajan,Neha Sarova,Jyoti Rustagi et al.
Deviyani Mahajan et al.
Background: Accurate early pregnancy detection is crucial for enhancing herd productivity in bovines. There is growing interest in identifying reliable protein biomarkers that can be used to develop rapid, sensitive, and ...
Extraction and characterization of functional duckweed proteins using alkaline, enzymatic, and ultrasound-assisted techniques [0.03%]
利用碱法、酶解及超声波辅助技术提取和表征功能水萍蛋白
Ayça Akyüz,Seda Ersus
Ayça Akyüz
The urgent need for sustainable, plant-based protein sources has brought aquatic crops like duckweed (Lemnaceae) into focus due to their high protein content, fast growth, and minimal resource requirements. This study aims to optimize and c...
A kaleidoscope of hosts: Expression systems of recombinant antibody reagents for immunological assays [0.03%]
用于免疫检测的重组抗体试剂的表达系统：多姿多彩的宿主细胞体系
Jia Xuan Yeoh,Yee Siew Choong,Theam Soon Lim
Jia Xuan Yeoh
Monoclonal antibodies (mAbs) have been shown to be highly promising reagents used in immunological analysis of various diseases and other chronic conditions due to their specific targeting, potency, and stability. Advances in protein engine...
H P Chethana,U Rathan Kumar,Gunimala Chakraborty et al.
H P Chethana et al.
Intrinsically disordered proteins/regions are abundant in cancer signalling pathways and neurodegenerative diseases like Parkinson's disease, amyotrophic lateral sclerosis, Alzheimer's disease, etc. Purification of intrinsically disordered ...
Amino acid chemistry and post-translational modifications underlying marine adhesive proteins: Biochemical insights for designing underwater adhesives [0.03%]
海洋粘附蛋白的氨基酸化学和翻译后修饰：设计水下粘合剂的生化启示
Taehee Yoon,Hyung Joon Cha
Taehee Yoon
Underwater adhesion remains a complex challenge due to the interference of water with traditional bonding mechanisms. Marine organisms, such as mussels, sandcastle worms, and barnacles, have evolved specialized adhesive proteins that overco...
Comparative biophysical and functional analysis of TCZ-UFRJ, a potential biosimilar to Actemra [0.03%]
TCZ-UFRJ的生物物理和功能特性分析及其与Actemra的比较研究
Sanclayver Corrêa Araújo,Federico Francisco Marsili,Renata Guimarães Alvim et al.
Sanclayver Corrêa Araújo et al.
Biosimilar antibodies have become increasingly significant in the pharmaceutical market, driven by the expiration of patents on many reference products. This study presents a comparative analysis between the originator tocilizumab, used to ...
Plasmid-transformed Bifidobacterium longum 105A secreting β-glucuronidase for prodrug conversion of SN-38 glucuronide [0.03%]
质粒转化产生的长双歧杆菌105A菌株分泌β-葡萄糖醛酸酶以将SN-38葡萄糖醛酸酯前药转化为治疗药物
Atsushi Saisho,Michiko Shimokawa,Rintaro Kubo et al.
Atsushi Saisho et al.
The development of tumor-selective prodrug activation strategies remains a major challenge in cancer pharmacotherapy. In this study, we focused on SN-38 glucuronide (SN-38G), a highly hydrophilic prodrug with low membrane permeability that ...