Comparative genomics of diverse Escherichia coli O157:H7 strains to characterize plasmids, prophages, virulence and antimicrobial resistance genes [0.03%]
比较基因组学在多样的大肠杆菌O157:H7菌株中表征质粒、前噬菌体、毒力和抗菌素抗性基因
Sydney Menzeko Gambushe,Peter Ayodeji Idowu,Oliver Tendayi Zishiri
Sydney Menzeko Gambushe
Plasmids play a critical role in bacterial evolution and represent major drivers of the emergence and dissemination of antimicrobial resistance. As primary mobile genetic elements (MGEs), plasmids facilitate the horizontal transfer of resis...
Megaplasmids of the enteropathogenic species Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus represent a group of novel genetic elements unrelated to other plasmids of Vibrionaceae [0.03%]
副溶血性弧菌、霍乱弧菌和创伤弧菌巨型质粒代表了一组新型的遗传元件，与其他 Vibrionaceae 质粒无关
Maria Borowiak,Jens A Hammerl,Eckhard Strauch
Maria Borowiak
Vibrio (V.) parahaemolyticus is a marine-associated bacterium that has previously been linked to foodborne illness associated with seafood consumption. Various plasmids harbouring antimicrobial resistance and virulence genes have been descr...
Assembly-based analysis of the infant gut microbiome reveals novel ubiquitous plasmids [0.03%]
基于基因组的婴儿肠道微生物组分析发现新的普遍存在质粒
Hanna Theodora Noordzij,Meike T Wortel,Anna Heintz-Buschart et al.
Hanna Theodora Noordzij et al.
Little is known about the role of mobile genetic elements in natural ecosystems such as the infant gut microbiome. Here, we conduct the most comprehensive longitudinal study of the infant plasmidome to date by analyzing monthly fecal sample...
Expression, purification, and refolding of an optimized SARS-CoV-2 receptor binding domain in E. coli [0.03%]
一种优化的新冠病毒受体结合区在大肠杆菌中的表达、纯化及复性
Anamika Biswas,Arighna Sarkar,Sreejith Raran-Kurussi et al.
Anamika Biswas et al.
The outbreak of Coronavirus Disease 2019 (COVID-19) has posed a significant risk to global health, warranting the formulation of efficient preventive and therapeutic measures to tackle its causative agent, severe acute respiratory syndrome ...
Single-strain mobilome sequencing quantifies bacterial genetic response to stress, including activity of IS elements, prophages, RNAs, and REPINs [0.03%]
单菌株移动基因组测序量化细菌遗传应激反应，包括IS元件、前噬菌体、RNA和REPIN的活性
Tue Kjærgaard Nielsen,Lars Hestbjerg Hansen
Tue Kjærgaard Nielsen
Microbial genomes are continuously being rearranged by mobile genetic elements (MGEs), leading to configurations that may confer novel phenotypic traits such as antibiotic resistance, degradation of compounds, or metabolic features. Standar...
Gloria Del Solar,Christopher M Thomas,Elisabeth Grohmann
Gloria Del Solar
Hideaki Nojiri,Masaki Shintani,Julian I Rood
Hideaki Nojiri
Insights from public database sequences related to the replication initiation protein TrfA of the IncP-1 plasmid RK2 [0.03%]
有关IncP-1质粒RK2复制起始蛋白TrfA的公共数据库序列的见解
Haruo Suzuki,Kazuki Moriguchi,Masaki Shintani et al.
Haruo Suzuki et al.
Replicon typing identifies sequences similar to known DNA replication initiators and is widely used to detect specific plasmid groups (e.g., IncP-1) in genome and metagenome sequencing data. However, the characteristics of these homologous ...
Prevention of RK2 plasmid replication initiation in starved Escherichia coli cells [0.03%]
饥饿条件下抑制大肠杆菌中RK2质粒的复制起始
Ewelina Boguszewska,Igor Konieczny
Ewelina Boguszewska
Bacterial adaptation to environmental stress involves stringent regulation of DNA replication. While the mechanisms controlling chromosomal replication under adverse conditions, such as amino acid starvation, are relatively well characteriz...
Nathan Fraikin,Bruria Samuel,David Burstein et al.
Nathan Fraikin et al.
Conjugative plasmids are key drivers of horizontal gene transfer and the spread of antimicrobial resistance. Their successful establishment in new hosts requires overcoming diverse bacterial defence mechanisms, such as restriction-modificat...