High-risk ST11 Klebsiella pneumoniae harboring blaKPC-2 in wildlife: Mobilome architecture and adaptive potential beyond hospital environments [0.03%]
具有blaKPC-2基因的高风险ST11克雷伯氏肺炎菌在野生动物中的可移动基因组结构与医院外适应性潜力研究
Carolina Aparecida Ramos,Stella Cabral,Joseane Cristina Ferreira et al.
Carolina Aparecida Ramos et al.
Carbapenem-resistant Klebsiella pneumoniae ST11 is a high-risk lineage predominantly associated with healthcare settings. Here, we report the phenotypic and genomic characterization of a KPC-producing K. pneumoniae ST11 isolate recovered fr...
High-risk ST11 Klebsiella pneumoniae harboring blaKPC-2 in wildlife: Mobilome architecture and adaptive potential beyond hospital environments [0.03%]
存在于野生动物中的高风险ST11肺炎克雷伯菌blaKPC-2携带株：医院外环境中的可移动基因组结构与适应性潜力
Carolina Aparecida Ramos,Stella Cabral,Joseane Cristina Ferreira et al.
Carolina Aparecida Ramos et al.
Carbapenem-resistant Klebsiella pneumoniae ST11 is a high-risk lineage predominantly associated with healthcare settings. Here, we report the phenotypic and genomic characterization of a KPC-producing K. pneumoniae ST11 isolate recovered fr...
Rational design of a thermo-inducible near-zero-background T-vector platform based on PhiX174 lysis gene E [0.03%]
基于PhiX174裂解基因E的近零背景热诱导T载体理性设计
Lixia Fu,Xiaodan Liu,Jianfeng Cao et al.
Lixia Fu et al.
T vectors are core tools for TA cloning and are widely used for the direct cloning of PCR products. In the present study, a novel versatile T vector, designated pZBT01C, was developed based on a modified lysis gene E (mE) from bacteriophage...
Enhanced resistance to macrolides and tetracyclines due to cooperation among plasmid-encoded genes associated with IS26 mobile genetic elements [0.03%]
与IS26可移动遗传元件相关的质粒编码基因的合作增强大环内酯类和四环素类抗生素的耐药性
David E Cummings,Mercedes I Coppola,Adele M Gargiulo et al.
David E Cummings et al.
Antibiotic resistance genes associated with IS26-family mobile genetic elements are globally distributed in the Gram-negative plasmidome. In many cases, antibiotic resistance genes are co-located with genes of unknown function. Two particul...
iBiT: A vector system allowing stable genome integration and scalable expression of NanoLuc fusion proteins to monitor dynamic protein-protein interactions in intact cells [0.03%]
一种允许稳定基因组整合和NanoLuc融合蛋白的可扩展表达以监测完整细胞中动态蛋白质-蛋白质相互作用的载体系统
Jan Dreute,M Lienhard Schmitz
Jan Dreute
The accurate determination of protein-protein interactions (PPIs) within living cells is essential for our understanding of cellular processes. Molecular interactions can be determined through complementation systems, in which the spatial p...
Comparative genomics of diverse Escherichia coli O157:H7 strains to characterize plasmids, prophages, virulence and antimicrobial resistance genes [0.03%]
比较基因组学在多样的大肠杆菌O157:H7菌株中表征质粒、前噬菌体、毒力和抗菌素抗性基因
Sydney Menzeko Gambushe,Peter Ayodeji Idowu,Oliver Tendayi Zishiri
Sydney Menzeko Gambushe
Plasmids play a critical role in bacterial evolution and represent major drivers of the emergence and dissemination of antimicrobial resistance. As primary mobile genetic elements (MGEs), plasmids facilitate the horizontal transfer of resis...
Megaplasmids of the enteropathogenic species Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus represent a group of novel genetic elements unrelated to other plasmids of Vibrionaceae [0.03%]
副溶血性弧菌、霍乱弧菌和创伤弧菌巨型质粒代表了一组新型的遗传元件，与其他 Vibrionaceae 质粒无关
Maria Borowiak,Jens A Hammerl,Eckhard Strauch
Maria Borowiak
Vibrio (V.) parahaemolyticus is a marine-associated bacterium that has previously been linked to foodborne illness associated with seafood consumption. Various plasmids harbouring antimicrobial resistance and virulence genes have been descr...
Assembly-based analysis of the infant gut microbiome reveals novel ubiquitous plasmids [0.03%]
基于基因组的婴儿肠道微生物组分析发现新的普遍存在质粒
Hanna Theodora Noordzij,Meike T Wortel,Anna Heintz-Buschart et al.
Hanna Theodora Noordzij et al.
Little is known about the role of mobile genetic elements in natural ecosystems such as the infant gut microbiome. Here, we conduct the most comprehensive longitudinal study of the infant plasmidome to date by analyzing monthly fecal sample...
Expression, purification, and refolding of an optimized SARS-CoV-2 receptor binding domain in E. coli [0.03%]
一种优化的新冠病毒受体结合区在大肠杆菌中的表达、纯化及复性
Anamika Biswas,Arighna Sarkar,Sreejith Raran-Kurussi et al.
Anamika Biswas et al.
The outbreak of Coronavirus Disease 2019 (COVID-19) has posed a significant risk to global health, warranting the formulation of efficient preventive and therapeutic measures to tackle its causative agent, severe acute respiratory syndrome ...
Single-strain mobilome sequencing quantifies bacterial genetic response to stress, including activity of IS elements, prophages, RNAs, and REPINs [0.03%]
单菌株移动基因组测序量化细菌遗传应激反应，包括IS元件、前噬菌体、RNA和REPIN的活性
Tue Kjærgaard Nielsen,Lars Hestbjerg Hansen
Tue Kjærgaard Nielsen
Microbial genomes are continuously being rearranged by mobile genetic elements (MGEs), leading to configurations that may confer novel phenotypic traits such as antibiotic resistance, degradation of compounds, or metabolic features. Standar...