Rational design of a thermo-inducible near-zero-background T-vector platform based on PhiX174 lysis gene E
{{output}}
T vectors are core tools for TA cloning and are widely used for the direct cloning of PCR products. In the present study, a novel versatile T vector, designated pZBT01C, was developed based on a modified lysis gene E (mE) from bacteriophage PhiX174, which allo... ...
