A functional investigation of antibody Fc-FcRn variant binding guided by in silico free energy perturbation methods [0.03%]
基于计算机自由能微扰方法的抗体Fc-FcRn变异结合功能研究
Jared M Sampson,Alina P Sergeeva,Tianyang Gao et al.
Jared M Sampson et al.
Accurate calculation of energy changes upon mutation is a key requirement for the effective use of computational methods in protein design. In this study, we applied free energy perturbation (FEP) calculations to predict the effects of muta...
Brain-seeded 42-residue amyloid-β fibrils that resemble fibrils directly extracted from Alzheimer's disease brain tissue [0.03%]
由阿尔茨海默病脑组织中直接提取的纤维素类似的42残基脑源淀粉样蛋白-β纤维体播种法
Kent R Thurber,Myungwoon Lee,Robert Tycko
Kent R Thurber
Fibrils formed by the 42-residue amyloid-β peptide (Aβ42) are known to be polymorphic, with molecular conformations and supramolecular structures that depend on conditions of nucleation, growth, seeding, or other factors. Structural studi...
Mapping interaction of assembly factor Rpn14 with the proteasome base reveals a bipartite interface and implies ordered remodeling of intersubunit contacts during proteasome biogenesis [0.03%]
解析装配因子RPN14与蛋白酶体底座的互作界面揭示了在蛋白酶体生物发生过程中亚基间相互作用的有序重塑现象
Quill Thomas,Madison Sterling,Lauren G Carnley et al.
Quill Thomas et al.
The 26S proteasome is the largest known protease and an essential mediator of targeted protein degradation, a transformative therapeutic modality for human diseases. Assembly of the 26S proteasome from its 66 cognate subunits depends on nin...
Towards a molecular understanding of the role of helix 8 in GPCR trafficking [0.03%]
关于G蛋白偶联受体转运中螺旋8作用的分子研究
Tommas Theiss Ehler Nielsen,Jan Hendrik Schmidt,Samir Mustafa et al.
Tommas Theiss Ehler Nielsen et al.
Helix 8 in G protein-coupled receptors (GPCRs) has recently been linked to receptor internalization (Schmidt et al.,2025, Sci. Adv. 11, eadv1499), but the molecular basis of this relationship remains unclear. Here, we examined a proposed me...
ADP-Bound States of Cytoplasmic Dynein: Cryo-electron Microscopy Reveals a Two-Step Post-Power-Stroke Transition and Roles of Regulatory ATPase Sites [0.03%]
细胞动力蛋白与ADP结合状态：低温电子显微镜揭示了功率冲程之后的两步转换及调节ATP酶位点的作用
Hiroshi Imai,Riko Kanazawa,Toshihisa Maeshima et al.
Hiroshi Imai et al.
Cytoplasmic dynein is essential for intracellular transport and cell division, and its dysfunction is implicated in severe neurological disorders. Its motility is driven by the primary ATPase site in the AAA1 module and allosterically modul...
Fabrice Lejeune,Yoon Ki Kim,Neeraj Sharma
Fabrice Lejeune
The Structure of Escherichia coli MscL and its dimer formation in Nanodiscs [0.03%]
大肠杆菌MscL的结构及其在纳米盘中的二聚体形成
Tim Rasmussen,Julia Isabel Bahner,Vanessa J Flegler et al.
Tim Rasmussen et al.
Mechanosensitive channels of large conductance (MscL) are essential bacterial safety valves that prevent osmotic lysis by releasing solutes in response to membrane tension. Despite extensive functional studies on Escherichia coli MscL (EcMs...
Genome-Wide Impact of Human DBR1 Depletion on RNA Processing Networks Reveal a Connection Between Pre-mRNA Splicing, mRNA Surveillance and Stress Granule Dynamics [0.03%]
人类DBR1缺失对RNA加工网络的基因组范围的影响揭示了前mRNA剪接，mRNA监视和压力颗粒动力学之间的联系
Tiffany Barwell,Caleb M Embree,Robert Reid et al.
Tiffany Barwell et al.
The RNA lariat debranching enzyme DBR1 is essential for intron turnover and RNA metabolism, yet its broader impact on transcriptome regulation remains incompletely defined. To elucidate the consequences of DBR1 depletion, we performed trans...
Deep Chaudhuri,Madhu Bhatt,Shubhasis Haldar
Deep Chaudhuri
Protein translocation across the bacterial SecYEG channel involves mechanical constraints arising from ATP-driven SecA activity, geometric confinement within the translocon, and folding of the emerging polypeptide on the periplasmic side. P...
Evolutionarily Conserved Interactions of RNA Polymerases with TFIIH via a Common Acidic Tail of the RPB6 Subunit [0.03%]
进化保守的RNA聚合酶通过RPB6亚基共同的酸性尾部与TFIIH相互作用
Masahiko Okuda,Yuriko Yoshimura,Aki Hayashi et al.
Masahiko Okuda et al.
In eukaryotes, the three RNA polymerases (RNAPs) share a flexible acidic N-terminal tail (NTT) derived from the common subunit RPB6. In human, NTT interacts with the PH domain (PH-D) of the p62 subunit in the general transcription/repair fa...