Expanding the Landscape of Disordered Flexible Linkers: A Structural and Computational Framework for DLD Dataset Assembly [0.03%]
无序柔性链接器的景观扩展：DLD数据集组装的结构和计算框架
Di Meng,Juliana Glavina,Heli M Garcia Alvarez et al.
Di Meng et al.
Disordered flexible linkers (DFLs) are functional elements found within intrinsically disordered regions that carry out key functions by connecting domains and/or short linear motifs. Understanding the features of DFLs is limited by the lac...
Codon Optimality Modulates Cellular Stress and Innate Immune Responses Triggered by Exogenous RNAs [0.03%]
密码子最优性调节外源RNA诱导的细胞应激和固有免疫反应
Chotiwat Seephetdee,Nada Bejar,Kathrina D Castillo et al.
Chotiwat Seephetdee et al.
Despite advancements in RNA sequence design, evidence regarding the preferential use of synonymous codons on cellular stress and innate immune responses is lacking. To this end, we developed a new codon optimality formula to re-engineer the...
Insights into Genome Ejection by a Therapeutic phiKMV-like Bacteriophage [0.03%]
治疗性φKMV样噬菌体基因组弹射的原理探讨
Junwei Li,Nathan F Bellis,Ravi K Lokareddy et al.
Junwei Li et al.
Ar-KM is a phiKMV-like therapeutic bacteriophage used in clinical candidate phage therapy cocktails to treat lung infections caused by P. aeruginosa. Here, we present an integrative structural atlas of Ar-KM proteins using cryo-EM, proteomi...
In Vivo Single-molecule Tracking of CHD Family Chromatin Remodeler Hrp3 Defines the Chromatin Context-dependent Binding Dynamics in S. pombe [0.03%]
S. pombe中CHD家族染色质重塑因子Hrp3的活细胞单分子追踪定义了染色质环境依赖性结合动力学
Akriti Kumari,Oankar Varde,Varsha Gopi et al.
Akriti Kumari et al.
ATP-dependent chromatin remodelers of the CHD family regulate genome organisation and transcription, yet their dynamic behaviour in native chromatin remains unclear. Here, we performed in vivo single-molecule tracking of the CHD remodeler H...
Coiled-Coil Domain Kinking Controls Laminin-332 Cleavage by Elastase [0.03%]
卷曲螺旋结构域的裂解控制弹性酶对层粘连蛋白-332的切割
Lucky Akter,Romain Amyot,Robert Großmann et al.
Lucky Akter et al.
Laminins are integral components of basement membranes, where they provide structural support and mediate integrin-dependent cell adhesion. A flexible coiled-coil domain forms the laminin long arm, but ultrastructural insight into this doma...
The GCN2-ATF4 signaling axis orchestrates the hepatic transcriptional stress response to short-term sulfur amino acid deprivation [0.03%]
短期硫氨基酸缺乏的肝脏转录应激反应由GCN2-ATF4信号轴调控
Valérie Carraro,Maëlle Cherpaz,Lucie Longechamp et al.
Valérie Carraro et al.
Mammals rely on the integrated stress response (ISR) to maintain essential amino acid (EAA) homeostasis. The kinase GCN2 is a key ISR sensor that is rapidly activated by uncharged tRNAs during EAA deprivation, leading to eIF2α phosphorylat...
URG7-Driven Homeostatic Adaptation Protects SH-SY5Y Cells from 6-OHDA Neurotoxicity [0.03%]
尿激酶型纤溶酶原激活物受体URG7通过稳态适应保护SH-SY5Y细胞免受6-羟多巴胺神经毒性损伤
Ilaria Nigro,Rocchina Miglionico,Ludovica Lela et al.
Ilaria Nigro et al.
Parkinson's disease (PD) is characterized by progressive dopaminergic neurodegeneration associated with oxidative stress, mitochondrial dysfunction, endoplasmic reticulum (ER) stress, and impaired proteostasis. In this study, we investigate...
New structures of human formylglycine-generating enzyme reveal features important for catalysis, disease and structure-based drug design [0.03%]
人来源酰甘氨醛生成酶的新结构揭示了对于催化、疾病和基于结构的药物设计重要的特征
Julia L Kowal,Sarfaraz Alam,Karthikeyan Radhakrishnan et al.
Julia L Kowal et al.
The formylglycine-generating enzyme (FGE) post-translationally modifies the active site of all human sulfatases. Mutations in the SUMF1 gene encoding FGE may lead to catalytically inactive FGE or destabilize the protein. The resulting lack ...
Effects of sequence reversal on the conformational dynamics, phase transitions, and function of Tau protein [0.03%]
序列倒置对Tau蛋白构象动力学、相变和功能的影响
Lumiao Wang,Yuhui Wang,Xi Wang et al.
Lumiao Wang et al.
Sequence reversal has been shown to significantly alter the folding and function of ordered proteins. Compared to ordered proteins, the conformations of intrinsically disordered proteins (IDPs) are more dynamic and expanded. Whether the equ...
How Topoisomerases Relax and Disentangle the Genome During DNA Replication [0.03%]
DNA复制过程中的拓扑异构酶基因组解旋和消散机制
James M Dewar
James M Dewar
DNA replication generates topological stress that must be relaxed and molecular entanglements that must be resolved. Unwinding of the parental duplex creates positive supercoils ahead of the fork, while fork rotation transfers parental inte...