Determination of equilibrium dissociation constants in fluorescence polarization [0.03%]
应用荧光偏振测定解离平衡常数
L Prystay,M Gosselin,P Banks
L Prystay
A simple mathematical model (the FP K(d) model) is used to generate the dissociation equilibrium constant (K(d)) for G protein-coupled receptor-ligand binding measured using fluorescence polarization (FP) saturation curve analysis. The mode...
S Fox,H Wang,L Sopchak et al.
S Fox et al.
Transfected cell lines as tools for high throughput screening: a call for standards [0.03%]
转染细胞系作为高通量筛选工具：建立标准的呼吁
L Pagliaro,M Praestegaard
L Pagliaro
During 1999, Journal of Biomolecular Screening presented a series of Point-Counterpoint articles that addressed a question posed by editor Bill Janzen: "What is the future of HTS?" These articles discussed many of the global issues involved...
Cost-effective whole-cell assay for laboratory evolution of hydroxylases in Escherichia coli [0.03%]
一种在大肠杆菌中进行羟基酶实验室进化的经济实用型全细胞筛选体系
U Schwaneberg,C Otey,P C Cirino et al.
U Schwaneberg et al.
Cytochrome P450 BM-3 from Bacillus megaterium catalyzes the subterminal hydroxylation of medium- and longchain fatty acids at the omega-1, omega-2, and omega-3 positions. A continuous spectrophotometric assay for P450 BM-3 based on the conv...
Development and use of a gene promoter-based screen to identify novel inhibitors of cyclooxygenase-2 transcription [0.03%]
开发和利用基于基因启动子的筛查来识别新的环氧化酶-2转录抑制剂
K Subbaramaiah,P Bulic,Y Lin et al.
K Subbaramaiah et al.
Cyclooxygenase-2 (COX-2) is a recognized target for cancer prevention and possibly treatment. To identify novel inhibitors of COX-2, we developed a high throughput reporter gene assay that utilizes a region of the human COX-2 promoter to dr...
Development of a miniaturized 384-well high throughput screen for the detection of substrates of cytochrome P450 2D6 and 3A4 metabolism [0.03%]
一种用于检测细胞色素P450 2D6和3A4代谢底物的微型化高通量筛选方法的发展：一篇技术报告
I Kariv,M P Fereshteh,K R Oldenburg
I Kariv
The identification of a large number of biologically active chemical entities during high throughput screening (HTS) necessitates the incorporation of new strategies to identify compounds with druglike properties early during the lead prior...
HTPS flow cytometry: a novel platform for automated high throughput drug discovery and characterization [0.03%]
HTPS流式细胞术：一种自动化的高通量药物发现与表征的新平台
B S Edwards,F W Kuckuck,E R Prossnitz et al.
B S Edwards et al.
The flow cytometer is unique among biomedical analysis instruments because it makes simultaneous and multiple optical measurements on individual cells or particles at high rates. High throughput flow cytometry represents a potentially impor...
Homogeneous cell-based fluorescence polarization assay for the direct detection of cAMP [0.03%]
均一细胞基质荧光偏振测定法对cAMP的直接检测
L Prystay,A Gagné,P Kasila et al.
L Prystay et al.
A fluorescence polarization-based functional assay for cyclic AMP (cAMP) production in cells has been proven effective for the detection of agonist-stimulated cAMP production in a HEK 293 recombinant cell line expressing the corticotropin-r...
K Moore,S Rees
K Moore
M Berg,K Undisz,R Thiericke et al.
M Berg et al.
Liquid handling in higher density microplates (e.g., 1536-well microplates) for more efficient drug screening necessitates carefully selected and optimized parameters. The quality of a liquid handling procedure is dependent on the carryover...
Comparative Study
Journal of biomolecular screening. 2001 Feb;6(1):47-56. DOI:10.1177/108705710100600107 2001