A microtiter assay for quantifying protein-protein interactions associated with cell-cell adhesion [0.03%]
一种用于定量与细胞粘附相关的蛋白质-蛋白质相互作用的微量滴定板测定法
Nicholas A Graham,Melissa D Pope,Tharathorn Rimchala et al.
Nicholas A Graham et al.
Cell-cell adhesions are a hallmark of epithelial tissues, and the disruption of these contacts plays a critical role in both the early and late stages of oncogenesis. The interaction between the transmembrane protein E-cadherin and the intr...
A novel cyclic adenosine monophosphate responsive luciferase reporter incorporating a nonpalindromic cyclic adenosine monophosphate response element provides optimal performance for use in G protein coupled receptor drug discovery efforts [0.03%]
一种新型环状鸟苷一磷酸反应型萤火虫荧光素酶报告基因纳入非回文环状鸟苷一磷酸反应元件可为G蛋白偶联受体药物发现工作提供最佳性能
Oleg G Chepurny,George G Holz
Oleg G Chepurny
The authors report the characterization of a novel cyclic adenosine monophosphate (cAMP)-responsive luciferase (Luc) reporter that exhibits optimal performance in high-throughput screens of agonist binding at G protein-coupled receptors (GP...
High-throughput screening by mass spectrometry: comparison with the scintillation proximity assay with a focused-file screen of AKT1/PKB alpha [0.03%]
基于质谱的高通量筛选及其与液闪计数聚焦库筛选AKT1/PKB alpha的比较研究
Andrea K Quercia,William A LaMarr,Jayhyuk Myung et al.
Andrea K Quercia et al.
Mass spectrometry is an emerging format for label-free high-throughput screening. The main limitation of mass spectrometry is throughput, due to the requirement to purify samples prior to ionization. Here the authors compare an automated hi...
Comparative Study
Journal of biomolecular screening. 2007 Jun;12(4):473-80. DOI:10.1177/1087057107300647 2007
Evaluation of different glutathione S-transferase-tagged protein captures for screening E6/E6AP interaction inhibitors using AlphaScreen [0.03%]
用于筛选E6/E6AP相互作用抑制剂的不同的谷胱甘肽S-转移酶标记的蛋白捕获法的评价研究(AlphaScreen法)
Peter Sehr,Michael Pawlita,Joe Lewis
Peter Sehr
Human papillomavirus (HPV) infection is responsible for the development of cervical cancer and its premalignant lesions in women. The virus-encoded oncogene E6 is a promising target for an anti-HPV drug therapy. The authors describe the dev...
Comparative Study
Journal of biomolecular screening. 2007 Jun;12(4):560-7. DOI:10.1177/1087057107301246 2007
Kyung-Sook Chung,Young-Joo Jang,Nam-Soon Kim et al.
Kyung-Sook Chung et al.
A total of 437 human full-length cDNAs isolated by microarray analysis of liver and/or gastric cancer tissues were evaluated for their relevance to cancer using the fission yeast Schizosaccharomyces pombe. Overexpression of 161 human cDNAs ...
Identifying druglike inhibitors of myelin-reactive T cells by phenotypic high-throughput screening of a small-molecule library [0.03%]
通过小分子文库的表型高通量筛选鉴定针对髓鞘T细胞的药物样抑制剂
Christina Rossi,Deepa Padmanaban,Jake Ni et al.
Christina Rossi et al.
Inflammatory T cells that are reactive to myelin protein components of the CNS play a critical role in the pathogenesis of multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). The authors have previ...
Genotyping of the lactase-phlorizin hydrolase c/t-13910 polymorphism by means of a new rapid denaturing high-performance liquid chromatography-based assay in healthy subjects and colorectal cancer patients [0.03%]
健康受试者和结直肠癌患者乳糖酶/芍药素水解酶基因型的新快速变性高效液相色谱检测方法
Ada Piepoli,Enrico Schirru,Angela Mastrorilli et al.
Ada Piepoli et al.
Adult-type hypolactasia results from the progressive decline of lactase-phlorizin hydrolase activity in enterocytes after weaning. Lactase nonpersistence may determine a primary lactose intolerance with reduced diary product consumption, wh...
Comparative Study
Journal of biomolecular screening. 2007 Aug;12(5):733-9. DOI:10.1177/1087057107301328 2007
Identification of a novel laser dye substrate of mammalian cytochromes P450: application in rapid kinetic analysis, inhibitor screening, and directed evolution [0.03%]
用于哺乳动物细胞色素P450的新型激光染料底物鉴定及其在快速动力学分析、抑制剂筛选和定向进化中的应用研究
Santosh Kumar
Santosh Kumar
The author sought to develop a high-throughput activity screening assay to carry out rapid kinetic analysis, inhibitor screening, and directed evolution of cytochrome P450 2C enzymes. Initially, of the 9 fluorescent substrates and 10 P450 2...
High-throughput flow cytometry-based assay to identify apoptosis-inducing proteins [0.03%]
一种用于识别促凋亡蛋白质的高通量流式细胞术测定法
Mamatha Sauermann,Florian Hahne,Christian Schmidt et al.
Mamatha Sauermann et al.
After sequencing the human genome, the challenge ahead is to systematically analyze the functions and disease relation of the proteins encoded. Here the authors describe the application of a flow cytometry-based high-throughput assay to scr...
High-throughput mass spectrometry screening for inhibitors of phosphatidylserine decarboxylase [0.03%]
磷脂酰丝氨酸去羧酶抑制剂的高通量质谱筛选技术
Chris D Forbes,Joshuaine G Toth,Can C Ozbal et al.
Chris D Forbes et al.
A high-throughput mass spectrometry assay to measure the catalytic activity of phosphatidylserine decarboxylase (PISD) is described. PISD converts phosphatidylserine to phosphatidylethanolamine during lipid synthesis. Traditional methods of...