Ian Burr,Toby Winchester,Wilma Keighley et al.
Ian Burr et al.
Codeveloping alongside chemistry and in vitro screening, compound management was one of the first areas in research recognizing the need for efficient processes and workflows. Material management groups have centralized, automated, miniatur...
Achieving accurate compound concentration in cell-based screening: validation of acoustic droplet ejection technology [0.03%]
声学滴定技术在基于细胞的高通量筛选中的应用及化合物浓度准确性评估
Richard John Grant,Karen Roberts,Carly Pointon et al.
Richard John Grant et al.
Compound handling is a fundamental and critical step in compound screening throughout the drug discovery process. Although most compound-handling processes within compound management facilities use 100% DMSO solvent, conventional methods of...
Characterization of a robust enzymatic assay for inhibitors of 2-oxoglutarate-dependent hydroxylases [0.03%]
一种稳健的基于酶的底物 assay 法筛查 2-酮戊二酸依赖性羟化酶抑制剂
Kimon C Kanelakis,Heather L Palomino,Lina Li et al.
Kimon C Kanelakis et al.
The prolyl-4-hydroxylase proteins regulate the hypoxia-inducible transcription factors (HIFs) by hydroxylation of proline residues targeting HIF-1alpha for proteasomal degradation. Using the purified catalytic domain of prolyl hydroxylase 2...
Long-term storage of compound solutions for high-throughput screening by using a novel 1536-well microplate [0.03%]
用于高通量筛选的新型1536孔微孔板的复方溶液的长期保存法
Martin Joseph Pfeifer,Guenther Scheel
Martin Joseph Pfeifer
This report describes the features and the performance of a new and significantly improved 1536-well microplate design. The design allows for simple, automation-friendly, and cost-effective storage of compound solutions for high-throughput ...
Design and implementation of an automated compound management system in support of lead optimization [0.03%]
一项支持先导化合物优化的自动化化合物管理系统的设计与实现
Catherine Quintero,Ilona Kariv
Catherine Quintero
To meet the needs of the increasingly rapid and parallelized lead optimization process, a fully integrated local compound storage and liquid handling system was designed and implemented to automate the generation of assay-ready plates direc...
Maximiliano Zaragoza-Sundqvist,Hans Eriksson,Mattias Rohman et al.
Maximiliano Zaragoza-Sundqvist et al.
Four years ago, the first acoustic droplet ejectors (ADEs) were launched on the market, providing a new generation of high-throughput noncontact liquid handlers that outclassed traditional contact instruments in almost every respect. This i...
Elizabeth Nixon,Sue Holland-Crimmin,Brian Lupotsky et al.
Elizabeth Nixon et al.
Since the introduction of lithotripsy kidney stone therapy, Focused Acoustics and its properties have been thoroughly utilized in medicine and exploration. More recently, Compound Management is exploring its applications and benefits to sam...
Advances in improving the quality and flexibility of compound management [0.03%]
提高化合物管理质量及灵活性的方法研究进展
William P Janzen,Ioana G Popa-Burke
William P Janzen
The process of drug discovery has evolved considerably since the advent of high-throughput screening (HTS) in the 1980s. Experts and opinion leaders today are agreeing that the current trend in the field is a focus on increasing overall qua...
Ian Sinclair,Isabel Charles
Ian Sinclair
Recent literature has described the exciting development of a new universal detection technology for high-performance liquid chromatography (HPLC), as well as some exploratory work on its application to quantitative measurement of solutes a...
Monitoring compound integrity with cytochrome P450 assays and qHTS [0.03%]
采用细胞色素P450检测试验和高通量定量筛选技术监测化合物的完整性
Ryan MacArthur,William Leister,Henrike Veith et al.
Ryan MacArthur et al.
The authors describe how room temperature storage of a 1120-member compound library prepared in either DMSO or in a hydrated-DMSO/water (67/33) mixture affects the reproducibility of potency values as monitored using cytochrome P450 1A2 and...