Characterization of a robust enzymatic assay for inhibitors of 2-oxoglutarate-dependent hydroxylases
{{output}}
The prolyl-4-hydroxylase proteins regulate the hypoxia-inducible transcription factors (HIFs) by hydroxylation of proline residues targeting HIF-1alpha for proteasomal degradation. Using the purified catalytic domain of prolyl hydroxylase 2 (PHD2(181-417)), an... ...
