C M Nunn,S Neidle
C M Nunn
The crystal structure of the self-complementary DNA octamer d(GGCATGCC) has been determined to a resolution of 2.38 A and R factor of 17.1%. The structure is an A-DNA octamer duplex with Watson-Crick base pairing along its length. This sequ...
Application of known triplet phases in the crystallographic study of bovine pancreatic trypsin inhibitor. I: studies at 1.55 and 1.75 a resolution [0.03%]
已知三重相在牛胰蛋白酶抑制剂晶体学研究中的应用。I. 1.55和1.75Å分辨率的研究
R H Mathiesen,F Mo
R H Mathiesen
The structure of bovine pancreatic trypsin inhibitor could be re-determined both with data at 1.55 and 1.75 A resolution using direct methods strengthened by the application of about 90 and 130 triplet phases, respectively, assumed known wi...
Crystallization of xylanase from Erwinia chrysanthemi: influence of heat and polymeric substrate [0.03%]
黄单胞菌木聚糖酶的结晶及其耐热性和底物特异性的研究
A P de la Rosa,J Day,S B Larson et al.
A P de la Rosa et al.
Xylanase from the bacterial plant pathogen Erwinia chrysanthemi (E.C. 3.2.1.8), expressed in E. coli, has been crystallized for X-ray diffraction analysis both in the presence and the absence of its polymeric substrate 4-O-methyl glucuronox...
G N Murshudov,A A Vagin,E J Dodson
G N Murshudov
This paper reviews the mathematical basis of maximum likelihood. The likelihood function for macromolecular structures is extended to include prior phase information and experimental standard uncertainties. The assumption that different par...
Partial improvement of crystal quality for microgravity-grown apocrustacyanin C1 [0.03%]
微重力培养的蓝藻素C1单晶品质的部分改善
E H Snell,A Cassetta,J R Helliwell et al.
E H Snell et al.
The protein apocrustacyanin C(1) has been crystallized by vapour diffusion in both microgravity (the NASA space shuttle USML-2 mission) and on the ground. Rocking width measurements were made on the crystals at the ESRF Swiss-Norwegian beam...
Isocitrate lyase from Aspergillus nidulans: crystallization and X-ray analysis of a glyoxylate cycle enzyme [0.03%]
来自曲霉的异柠檬酸裂合酶：乙醛酸循环酶的结晶和X射线分析
S J Langridge,P J Baker,J R DeLucas et al.
S J Langridge et al.
Isocitrate lyase (ICL) from the filamentous fungus Aspergillus nidulans catalyzes the first committed step of the carbon-conserving glyoxylate bypass. This enzyme has been crystallized by the hanging-drop method of vapour diffusion using po...
Purification, crystallization and preliminary X-ray analysis of Bacteroides fragilis Zn2+ beta-lactamase [0.03%]
脆弱类杆菌锌依赖性β-内酰胺酶的纯化、结晶及初步X射线分析
A Carfí,R Paul-Soto,L Martin et al.
A Carfí et al.
The Zn(2+) beta-lactamase from Bacteroides fragilis (E.C. 3.5.2.6) was overexpressed in Escherichia coli using an isopropylthiogalactoside-inducible T7 RNA polymerase expression system. Crystallization trials by the hanging-drop vapour-diff...
Crystallization of the RNA guanylyltransferase of Chlorella virus PBCV-1 [0.03%]
绿藻病毒PBCV-1的RNA鸟苷基转移酶的结晶化研究
A J Doherty,K Håkansson,C K Ho et al.
A J Doherty et al.
RNA guanylyltransferase, or capping enzyme (E.C. 2.7.7.50) catalyzes the transfer of GMP from GTP to diphosphate-terminated RNA to form the cap structure GpppN. Chlorella virus capping enzyme expressed in E. coli has been purified, treated ...
Preliminary crystallographic study on a low molecular weight form of bacterial plasminogen activator staphylokinase [0.03%]
细菌纤溶酶原激活物表皮葡萄球菌激酶的初步结晶学研究
D Chattopadhyay,J E Stewart,C D Smith et al.
D Chattopadhyay et al.
Staphylokinase, a 17 kDa protein, produced by certain strains of Staphylococcus aureus functions as a fibrin-specific plasminogen activator. During its interaction with plasminogen, staphylokinase is converted into a low molecular weight fo...
Purification, crystallization and preliminary X-ray analysis of the M.BseCI DNA methyltransferase from Bacillus stearothermophilus [0.03%]
来自卵形芽孢杆菌的M.BseCI DNA甲基化酶的纯化、结晶及初步X射线分析
A Athanasiadis,Y Papanikolau,M Rina et al.
A Athanasiadis et al.
The DNA methyltransferase M.BseC1 from B. stearothermophilus methylates the N6 atom of the 3' adenine in the sequence 5'-ATCGAT-3'. The 579-residue protein has been isolated and crystallized using seeding and microdialysis techniques. The c...