Purification, crystallization and preliminary X-ray analysis of Bacteroides fragilis Zn2+ beta-lactamase
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The Zn(2+) beta-lactamase from Bacteroides fragilis (E.C. 3.5.2.6) was overexpressed in Escherichia coli using an isopropylthiogalactoside-inducible T7 RNA polymerase expression system. Crystallization trials by the hanging-drop vapour-diffusion method have yi... ...
