Karolina Michalska,Dominika Borek,Alejadra Hernández-Santoyo et al.
Karolina Michalska et al.
Plant-type L-asparaginases hydrolyze the side-chain amide bond of L-asparagine or its beta-peptides. They belong to the N-terminal nucleophile (Ntn) hydrolases and are synthesized as inactive precursor molecules. Activation occurs via the a...
Critical importance of loop conformation to avidin-enhanced hydrolysis of an active biotin ester [0.03%]
环状构象对亲和素增强活性生物素酯水解的重要性critical importance of loop conformation to avidin-enhanced hydrolysis of an active biotin ester
Ruchama Hayouka,Yael Eisenberg-Domovich,Vesa P Hytönen et al.
Ruchama Hayouka et al.
The homotetrameric and biotin-binding properties of avidin and streptavidin have been exploited for a myriad of biotechnological applications and theoretical studies. Among the few differences between the two proteins is the capacity of avi...
Glycerol concentrations required for the successful vitrification of cocktail conditions in a high-throughput crystallization screen [0.03%]
高通量结晶筛选中甘油浓度对鸡尾酒条件玻璃化的成功要求
Robin Kempkes,Elizabeth Stofko,Kam Lam et al.
Robin Kempkes et al.
The Hauptman-Woodward Medical Research Institute runs a high-throughput crystallization screening service in which macromolecules are screened against 1536 potential crystallization cocktails. Typically, multiple crystallization leads are i...
Tobias Karlberg,Ruairi Collins,Susanne van den Berg et al.
Tobias Karlberg et al.
Argininosuccinate synthetase catalyzes the transformation of citrulline and aspartate into argininosuccinate and pyrophosphate using the hydrolysis of ATP to AMP and pyrophosphate. This enzymatic process constitutes the rate-limiting step i...
Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13) [0.03%]
人单核细胞趋化蛋白4(MCP-4/CCL13)的结构
Cyril Barinka,Adam Prahl,Jacek Lubkowski
Cyril Barinka
Monocyte chemoattractant proteins (MCPs) belong to the CC chemokine family and are involved in many (patho)physiological processes characterized by mononuclear cell infiltration, including tissue remodeling, atherosclerosis and cancer metas...
Differential specific radiation damage in the Cu II-bound and Pd II-bound forms of an alpha-helical foldamer: a case study of crystallographic phasing by RIP and SAD [0.03%]
铜(II)和钯(II)络合的α-螺旋嵌段聚合物的辐射损伤差异：一种RIP和SAD衍射相位确定的案例研究
Klaus Fütterer,Raimond B G Ravelli,Scott A White et al.
Klaus Fütterer et al.
The high photon flux at third-generation synchrotron sources can inflict significant primary radiation damage upon macromolecular crystals, even when the crystals are cryocooled. However, specific radiation-induced structural changes can be...
Metals in proteins: correlation between the metal-ion type, coordination number and the amino-acid residues involved in the coordination [0.03%]
蛋白质中的金属离子：金属离子类型、配位数及参与配位的氨基酸残基之间的关系
Ivan Dokmanić,Mile Sikić,Sanja Tomić
Ivan Dokmanić
Metal ions are constituents of many metalloproteins, in which they have either catalytic (metalloenzymes) or structural functions. In this work, the characteristics of various metals were studied (Cu, Zn, Mg, Mn, Fe, Co, Ni, Cd and Ca in pr...
Three-dimensional structures of L-asparaginase from Erwinia carotovora complexed with aspartate and glutamate [0.03%]
来源于欧文氏菌的L-天冬酰胺酶与L-天冬氨酸和谷氨酸结合的复合物的三维结构
O V Kravchenko,Yu A Kislitsin,A N Popov et al.
O V Kravchenko et al.
The crystal structures of Erwinia carotovora L-asparaginase complexed with L-aspartate and L-glutamate were determined at 1.9 and 2.2 A, respectively, using the molecular-replacement method and were refined to R factors of about 21% in both...
Application of maximum-entropy maps in the accurate refinement of a putative acylphosphatase using 1.3 A X-ray diffraction data [0.03%]
最大熵图在1.3埃分辨率衍射数据上的酰基磷at酶同源模的精确优化-refinement of a putative acylphosphatase using 1.3 A X-ray diffraction data
Eiji Nishibori,Takahiro Nakamura,Masanori Arimoto et al.
Eiji Nishibori et al.
Accurate structural refinement of a putative acylphosphatase using 1.3 A X-ray diffraction data was carried out using charge densities determined by the maximum-entropy method (MEM). The MEM charge density clearly revealed detailed features...
Modulation of activity by Arg407: structure of a fungal alpha-1,2-mannosidase in complex with a substrate analogue [0.03%]
Arg407调节活性：与底物类似物结合的真菌α-1，2-甘露糖苷酶结构
Yuri D Lobsanov,Takashi Yoshida,Tom Desmet et al.
Yuri D Lobsanov et al.
Class I alpha-mannosidases (glycoside hydrolase family GH47) play key roles in the maturation of N-glycans and the ER-associated degradation of unfolded glycoproteins. The 1.95 A resolution structure of a fungal alpha-1,2-mannosidase in com...