Ultralow-resolution ab initio phasing of filamentous proteins: crystals from a six-Ig fragment of titin as a case study [0.03%]
超高分辨率从头相位测定ofilamentous蛋白质的晶体：titin六Ig片段为例研究
Alexandre Urzhumtsev,Eleonore von Castelmur,Olga Mayans
Alexandre Urzhumtsev
Low-resolution diffraction data (resolution below 12 angstroms) from crystals of a filamentous six-Ig fragment of titin, I65-I70, were used in ab initio phasing with the aim of calculating its lattice packing and molecular envelope. Filamen...
Impact of low-frequency hotspot mutation R282Q on the structure of p53 DNA-binding domain as revealed by crystallography at 1.54 angstroms resolution [0.03%]
低频热点突变R282Q对p53 DNA结合结构域的影响：1.54埃分辨率晶体学表征
Chao Tu,Yu Hong Tan,Gary Shaw et al.
Chao Tu et al.
Tumor suppressor p53 is a sequence-specific DNA-binding protein and its central DNA-binding domain (DBD) harbors six hotspots (Arg175, Gly245, Arg248, Arg249, Arg273 and Arg282) for human cancers. Here, the crystal structure of a low-freque...
Structure of acostatin, a dimeric disintegrin from Southern copperhead (Agkistrodon contortrix contortrix), at 1.7 A resolution [0.03%]
南方铜头蛇双链离整合素蛋白质结构解析
Natalia Moiseeva,Robert Bau,Stephen D Swenson et al.
Natalia Moiseeva et al.
Disintegrins are a family of small (4-14 kDa) proteins that bind to another class of proteins, integrins. Therefore, as integrin inhibitors, they can be exploited as anticancer and antiplatelet agents. Acostatin, an alphabeta heterodimeric ...
Multiple crystal structures of actin dimers and their implications for interactions in the actin filament [0.03%]
肌动蛋白二聚体的多种晶体结构及其对肌动蛋白纤维中相互作用的意义
Michael R Sawaya,D S Kudryashov,Inna Pashkov et al.
Michael R Sawaya et al.
The structure of actin in its monomeric form is known at high resolution, while the structure of filamentous F-actin is only understood at considerably lower resolution. Knowing precisely how the monomers of actin fit together would lead to...
Structure of peptidyl-tRNA hydrolase 2 from Pyrococcus horikoshii OT3: insight into the functional role of its dimeric state [0.03%]
来自于Pyrococcus horikoshii OT3的肽基- tRNA水解酶2的结构：其二聚体形式功能作用的新见解
Katsumi Shimizu,Chizu Kuroishi,Mitsuaki Sugahara et al.
Katsumi Shimizu et al.
Peptidyl-tRNA hydrolases catalyze the hydrolytic removal of the peptidyl moiety from the peptidyl-tRNA molecule to prevent misreading during translation. Here, the expression, purification, crystallization and X-ray diffraction study of pep...
Qiang Zhao,Qing Han,Charles R Kissinger et al.
Qiang Zhao et al.
The hepatitis C (HCV) internal ribosome entry site (IRES) element plays a central role in cap-independent translation of the viral genomic RNA. The unique conformation of IRES domain II is critical for 80S ribosomal assembly and initiation ...
A 7μm mini-beam improves diffraction data from small or imperfect crystals of macromolecules [0.03%]
7微米细光束改善了小分子或不完美晶体的衍射数据
Ruslan Sanishvili,Venugopalan Nagarajan,Derek Yoder et al.
Ruslan Sanishvili et al.
A simple apparatus for achieving beam sizes in the range 5-10 μm on a synchrotron beamline was implemented in combination with a small 125 x 25 μm focus. The resulting beam had sufficient flux for crystallographic data collection from sam...
A knowledge-driven approach for crystallographic protein model completion [0.03%]
一种知识驱动的蛋白质晶体学模型补充方法
Krista Joosten,Serge X Cohen,Paul Emsley et al.
Krista Joosten et al.
One of the most cumbersome and time-demanding tasks in completing a protein model is building short missing regions or ;loops'. A method is presented that uses structural and electron-density information to build the most likely conformatio...
A mixture of fortunes: the curious determination of the structure of Escherichia coli BL21 Gab protein [0.03%]
奇特的决定：大肠杆菌BL21脱氨酶辅因子蛋白结构的确立
Bernhard Lohkamp,Doreen Dobritzsch
Bernhard Lohkamp
In protein crystallography, monodisperse protein samples of high purity are usually required in order to obtain diffraction-quality crystals. Here, crystals were reproducibly grown from a protein sample before its homogeneity had been deter...
Structures of two archaeal diphthine synthases: insights into the post-translational modification of elongation factor 2 [0.03%]
两类古菌二磷噻唑氨基酸合成酶的结构研究及对延伸因子2翻译后修饰的启示
Seiichiro Kishishita,Katsumi Shimizu,Kazutaka Murayama et al.
Seiichiro Kishishita et al.
The target of diphtheria toxin is the diphthamide residue in translation elongation factor 2 (EF-2), which is generated by a three-step post-translational modification of a specific histidine residue in the EF-2 precursor. In the second mod...