Charge-density analysis of a protein structure at subatomic resolution: the human aldose reductase case [0.03%]
人醛糖还原酶结构的次原子分辨率电荷密度分析
Benoît Guillot,Christian Jelsch,Alberto Podjarny et al.
Benoît Guillot et al.
The valence electron density of the protein human aldose reductase was analyzed at 0.66 angstroms resolution. The methodological developments in the software MoPro to adapt standard charge-density techniques from small molecules to macromol...
A new rotation function for molecular replacement by using both the self and cross Patterson vectors [0.03%]
一种新的旋转函数：利用自旋和交叉帕特森向量进行分子替代
Fan Jiang
Fan Jiang
The rotation function in molecular replacement is traditionally computed in reciprocal space. The common practice is to use a Patterson vector radius of about the size of the target molecule to limit the interatomic vectors to match only th...
Unique features of the structure and interactions of mycobacterial uracil-DNA glycosylase: structure of a complex of the Mycobacterium tuberculosis enzyme in comparison with those from other sources [0.03%]
结核分枝杆菌尿嘧啶-DNA糖基化酶结构和相互作用的特征：与其它物种相应蛋白结构的比较
Prem Singh Kaushal,Ramappa K Talawar,P D V Krishna et al.
Prem Singh Kaushal et al.
Uracil-DNA glycosylase (UNG), a repair enzyme involved in the excision of uracil from DNA, from mycobacteria differs from UNGs from other sources, particularly in the sequence in the catalytically important loops. The structure of the enzym...
Structure of a rabbit muscle fructose-1,6-bisphosphate aldolase A dimer variant [0.03%]
兔肌肉果糖-1,6-二磷酸醛缩酶A变异体二聚体的结构
Manashi Sherawat,Dean R Tolan,Karen N Allen
Manashi Sherawat
Fructose-1,6-bisphosphate aldolase (aldolase) is an essential enzyme in glycolysis and gluconeogenesis. In addition to this primary function, aldolase is also known to bind to a variety of other proteins, a property that may allow it to per...
Structure of monkey dimeric dihydrodiol dehydrogenase in complex with isoascorbic acid [0.03%]
猕猴二聚体二氢二醇脱氢酶与异抗坏血酸复合物的结构
Vincenzo Carbone,Rie Sumii,Shuhei Ishikura et al.
Vincenzo Carbone et al.
Mammalian dimeric dihydrodiol dehydrogenase (DD) is identical to NADP+-dependent D-xylose dehydrogenase. A recent investigation showed that the three-dimensional structure of monkey DD is similar to those of prokaryotic NADP(H)-dependent gl...
A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry [0.03%]
一种具有二十九倍二面体对称性的 Vault 核糖核蛋白颗粒
Koji Kato,Hideaki Tanaka,Tomoyuki Sumizawa et al.
Koji Kato et al.
Vault is a 12.9 MDa ribonucleoprotein particle with a barrel-like shape, two protruding caps and an invaginated waist structure that is highly conserved in a wide variety of eukaryotes. Multimerization of the major vault protein (MVP) is su...
Iterative-build OMIT maps: map improvement by iterative model building and refinement without model bias [0.03%]
迭代构建OMIT图：通过反复建模和精炼改进地图而没有模型偏差
Thomas C Terwilliger,Ralf W Grosse-Kunstleve,Pavel V Afonine et al.
Thomas C Terwilliger et al.
A procedure for carrying out iterative model building, density modification and refinement is presented in which the density in an OMIT region is essentially unbiased by an atomic model. Density from a set of overlapping OMIT regions can be...
Rajendrakumar A Gosavi,Timothy C Mueser,Constance A Schall
Rajendrakumar A Gosavi
Increasing the solubility of protein stock solutions to above that in a standard chromatography buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl) led to an increase in the number of crystallization conditions for ten globular proteins subjected t...
The structures of Alcaligenes faecalis D-3-hydroxybutyrate dehydrogenase before and after NAD+ and acetate binding suggest a dynamical reaction mechanism as a member of the SDR family [0.03%]
芽孢杆菌D-羟基丁酸脱氢酶的晶体结构表明其作为SDR家族成员具有动态反应机制
Md Mominul Hoque,Satoru Shimizu,Md Tofazzal Hossain et al.
Md Mominul Hoque et al.
D-3-Hydroxybutyrate dehydrogenase, which catalyzes the reversible reaction between D-3-hydroxybutyrate and acetoacetate, has been classified into the short-chain dehydrogenase/reductase family and is a useful marker in the assay of diabetes...
Use of complementary cation and anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1 [0.03%]
利用互补的阳离子和阴离子重原子盐衍生物确定细胞色素P450 46A1结构
Mark Andrew White,Natalia Mast,Ingemar Bjorkhem et al.
Mark Andrew White et al.
Human cytochrome P450 46A1 (CYP46A1) is one of the key enzymes in cholesterol homeostasis in the brain. The crystallization and heavy-atom structure solution of an active truncated CYP46A1 in complex with the high-affinity substrate analogu...