Rapid shape determination of tissue transglutaminase using high-throughput computing [0.03%]
利用高性能计算快速测定组织转谷氨酰胺酶的结构
Donna Lammie,James Osborne,Daniel Aeschlimann et al.
Donna Lammie et al.
Small-angle X-ray scattering can be used to determine the molecular shape of macromolecules in solution which are otherwise refractory to conventional high-resolution studies. DAMMIN and GASBOR are applications that utilize ab initio method...
Effect of a sodium ion on the dehydration-induced phase transition of monoclinic lysozyme crystals [0.03%]
钠离子对单斜形溶菌酶晶体脱水诱导相变的影响研究
Kazuaki Harata,Toshihiko Akiba
Kazuaki Harata
A monoclinic lysozyme crystal grown in NaCl solution was transformed into a new monoclinic crystal form by controlled dehydration. This crystal-to-crystal phase transition was accompanied by 20-40% solvent loss and the transformed crystal d...
Justyna Korczyńska,Ting Chou Hu,David K Smith et al.
Justyna Korczyńska et al.
The development of new crystallization platforms via the application of high-throughput technologies has delivered a plethora of crystallization plates suitable for robot-driven and manual setups. However, practically all these plates (exce...
The effect of deuteration on protein structure: a high-resolution comparison of hydrogenous and perdeuterated haloalkane dehalogenase [0.03%]
氘标记对蛋白质结构的影响——以卤代烷脱卤酶为例的高分辨率氢/氘对比研究
Xuying Liu,B Leif Hanson,Paul Langan et al.
Xuying Liu et al.
Haloalkane dehalogenase from Xanthobacter autotrophicus (XaDHL) was overexpressed under different isotopic conditions to produce fully hydrogenous (h-XaDHL) and perdeuterated (d-XaDHL) enzyme forms. Deuterium atoms at labile positions were ...
An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer [0.03%]
斜方晶系Dicer冰晶体中假同类孪晶的一个特殊例子
Ian J MacRae,Jennifer A Doudna
Ian J MacRae
The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from cry...
Structure of GES-1 at atomic resolution: insights into the evolution of carbapenamase activity in the class A extended-spectrum beta-lactamases [0.03%]
GES-1的原子结构解析：类A广谱β-内酰胺酶卡巴 penemase活性进化机制的新见解
Clyde A Smith,Marisa Caccamo,Katherine A Kantardjieff et al.
Clyde A Smith et al.
The structure of the class A extended-spectrum beta-lactamase GES-1 from Klebsiella pneumoniae has been determined to 1.1 A resolution. GES-1 has the characteristic active-site disulfide bond of the carbapenemase family of beta-lactamases a...
Structural studies of a bifunctional inhibitor of neprilysin and DPP-IV [0.03%]
NEP/DPP-4双功能抑制剂的结构研究
Christian Oefner,Sabine Pierau,Henk Schulz et al.
Christian Oefner et al.
Neutral endopeptidase (NEP) is the major enzyme involved in the metabolic inactivation of a number of bioactive peptides including the enkephalins, substance P, endothelin, bradykinin and atrial natriuretic factor, as well as the incretin h...
The structure of apo tryptophanase from Escherichia coli reveals a wide-open conformation [0.03%]
来自大肠杆菌的无辅因子色氨酸酶的结构展示了一种开放式的构象
Natalia Tsesin,Anna Kogan,Garik Y Gdalevsky et al.
Natalia Tsesin et al.
The crystal structure of apo tryptophanase from Escherichia coli (space group F222, unit-cell parameters a = 118.4, b = 120.1, c = 171.2 A) was determined at 1.9 A resolution using the molecular-replacement method and refined to an R factor...
Production of Slit2 LRR domains in mammalian cells for structural studies and the structure of human Slit2 domain 3 [0.03%]
用于结构研究的人Slit2 LRR结构城的哺乳动物细胞表达及人类Slit2第三结构域的结构分析
Cecile Morlot,Wieger Hemrika,Roland A Romijn et al.
Cecile Morlot et al.
Slit2 and Roundabout 1 (Robo1) provide a key ligand-receptor interaction for the navigation of commissural neurons during the development of the central nervous system. Slit2 is a large multidomain protein containing an unusual domain organ...
Photoreduction of the active site of the metalloprotein putidaredoxin by synchrotron radiation [0.03%]
利用同步辐射照射金属酶蛋白_PUTIDAREDOXIN活性中心的光还原反应研究
Mary C Corbett,Matthew J Latimer,Thomas L Poulos et al.
Mary C Corbett et al.
X-ray damage to protein crystals is often assessed on the basis of the degradation of diffraction intensity, yet this measure is not sensitive to the rapid changes that occur at photosensitive groups such as the active sites of metalloprote...