Structure of sylvaticin, a new alpha-elicitin-like protein from Pythium sylvaticum [0.03%]
来自Pythium sylvaticum的新α-elicin类似蛋白sylvaticin的结构
Marie-Bernard Lascombe,Pascal Retailleau,Michel Ponchet et al.
Marie-Bernard Lascombe et al.
The structure of sylvaticin, a 10 kDa major pythin protein excreted by the parasitic oomycete Pythium sylvaticum, has been determined. Although closely related to alpha-elicitins in its biological response, toxicity and overall structure, s...
X-ray crystal structural analysis of the binding site in the ferric and oxyferrous forms of the recombinant heme dehaloperoxidase cloned from Amphitrite ornata [0.03%]
重组线虫虫茧变形虫血红素脱卤过氧化物酶的铁离子和一氧化铁离子形式结合位点的X射结晶体结构分析
Vesna de Serrano,Zuxu Chen,Michael F Davis et al.
Vesna de Serrano et al.
The dehaloperoxidase (DHP) from the terebellid polychaete Amphitrite ornata is an enzyme that converts para-halogenated phenols to the corresponding quinones in the presence of hydrogen peroxide. Its enzymatic activity is similar to that of...
Refined crystal structures of red and green fluorescent proteins from the button polyp Zoanthus [0.03%]
来自纽扣水母Zoanthus的红色和绿色荧光蛋白的精细晶体结构
Nadezhda Pletneva,Vladimir Pletnev,Tamara Tikhonova et al.
Nadezhda Pletneva et al.
The three-dimensional structures of the wild-type red (zRFP574) and green (zGFP506) fluorescent proteins (FP) from the button polyp Zoanthus have been determined at 1.51 and 2.2 A resolution, respectively. In addition, the crystal structure...
Conformational switches in winged-helix domains 1 and 2 of bacterial translation elongation factor SelB [0.03%]
细菌翻译延长因子SelB翼状螺旋结构域1和2中的构象转换
Oleg Ganichkin,Markus C Wahl
Oleg Ganichkin
The crystal structure of the first two winged-helix motifs of translation elongation factor SelB from Moorella thermoacetica has been determined at 1.1 A resolution. Compared with the previous structure of the two domains in conjunction wit...
Structure determination of the O-methyltransferase NovP using the 'free lunch algorithm' as implemented in SHELXE [0.03%]
利用SHELXE中的“免费午餐算法”确定O-甲基转移酶NovP的结构
Isabel Usón,Clare E M Stevenson,David M Lawson et al.
Isabel Usón et al.
NovP is an S-adenosyl-L-methionine-dependent O-methyltransferase from Streptomyces spheroides (subunit MW = 29 967 Da). Recombinant N-terminally His-tagged NovP crystallizes in space group P2, with approximate unit-cell parameters a = 51.81...
Structure of an archaeal TYW1, the enzyme catalyzing the second step of wye-base biosynthesis [0.03%]
一个古菌TYW1的结构，催化wye型碱基生物合成第二步反应酶
Sakurako Goto-Ito,Ryohei Ishii,Takuhiro Ito et al.
Sakurako Goto-Ito et al.
Wye bases are tricyclic bases that are found in archaeal and eukaryotic tRNAs. The most modified wye base, wybutosine, which appears at position 37 (the 3'-adjacent position to the anticodon), is known to be important for translational read...
Structure of an aliphatic amidase from Geobacillus pallidus RAPc8 [0.03%]
来自Geobacillus pallidus RAPc8的烷基胺酶结构
Serah W Kimani,Vinod B Agarkar,Donald A Cowan et al.
Serah W Kimani et al.
The amidase from Geobacillus pallidus RAPc8, a moderate thermophile, is a member of the nitrilase superfamily and catalyzes the conversion of amides to the corresponding carboxylic acids and ammonia. It shows both amide-hydrolysis and acyl-...
Structure of the nondiscriminating aspartyl-tRNA synthetase from the crenarchaeon Sulfolobus tokodaii strain 7 reveals the recognition mechanism for two different tRNA anticodons [0.03%]
来自栖热菌株7的非识别天冬氨酸氨酰-TRNA合成酶结构揭示了两种不同反密码子的识别机制
Yoshiteru Sato,Yohei Maeda,Satoru Shimizu et al.
Yoshiteru Sato et al.
In protein synthesis, 20 types of aminoacyl-tRNA synthetase (aaRS) are generally required in order to distinguish between the 20 types of amino acid so that each achieves strict recognition of the cognate amino acid and the cognate tRNA. In...
Structure-factor extrapolation using the scalar approximation: theory, applications and limitations [0.03%]
标量近似结构因子外推：理论、应用及局限性
Ulrich K Genick
Ulrich K Genick
For many experiments in macromolecular crystallography, the overall structure of the protein/nucleic acid is already known and the aim of the experiment is to determine the effect a chemical or physical perturbation/activation has on the st...
Using barium ions for heavy-atom derivatization and phasing of xylanase II from Trichoderma longibrachiatum [0.03%]
用钡离子对长枝木霉半纤维素酶Ⅱ进行重金属衍生和相位确定
Natalia Moiseeva,Marc Allaire
Natalia Moiseeva
This paper describes the use of barium chloride to produce a heavy-atom derivative of xylanase II crystals from Trichoderma longibrachiatum, which was obtained either by cocrystallization or soaking. SAD phasing led to interpretable electro...