Localization by immunogold cytochemistry of the virus-coded 30K protein in plasmodesmata of leaves infected with tobacco mosaic virus [0.03%]
烟草花叶病毒感染的叶片中病毒编码的30K蛋白在胞间连丝中的定位研究:免疫金细胞化学法
K Tomenius,D Clapham,T Meshi
K Tomenius
The 30K protein of tobacco mosaic virus (TMV) was localized to the plasmodesmata of infected tobacco leaves by immunogold cytochemistry. This protein has been reported to be in the nuclear fraction of TMV-infected protoplasts, but as it has...
Processing of the tobacco etch virus 49K protease requires autoproteolysis [0.03%]
烟草蚀刻病毒49K蛋白酶的加工需要自水解作用
J C Carrington,W G Dougherty
J C Carrington
The final products encoded by the tobacco etch virus genome arise by proteolytic cleavage of a single large polyprotein precursor. Processing of the polyprotein at several sites requires the activity of a viral protease of 49,000 molecular ...
D Zuidema,L A Heaton,A O Jackson
D Zuidema
The structure of the gene adjacent to the "leader RNA" gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, was deduced by dideoxyribonucleotide sequence analysis of SYNV genomic (g) RNA and a series of plasmids constructed from SY...
C Masuta,D Zuidema,B G Hunter et al.
C Masuta et al.
The relatedness of the genomes of satellite panicum mosaic virus (SPMV) and its helper virus, panicum mosaic virus (PMV), were investigated by nucleic acid hybridization. The results show that the satellite and helper virus RNAs have no app...
Site-specific cleavage and religation of viral RNAs. I. Infectivity of barley stripe mosaic virus RNA religated from functionally active segments and restoration of the internal poly(A) tract in progeny [0.03%]
病毒RNA的定点切割和连接。I. 小麦条纹花叶病毒功能片段重新连接的RNA的感染性和子代体内多聚(A)序列的恢复
L G Tyulkina,O V Karpova,N P Rodionova et al.
L G Tyulkina et al.
The genomic RNAs of barley stripe mosaic virus (BSMV) were used in experiments involving site-specific cleavage to yield noninfective but functionally active fragments and subsequent restoration of infectious viral RNA by means of religatio...
Expression of alfalfa mosaic virus and tobacco rattle virus coat protein genes in transgenic tobacco plants [0.03%]
转基因烟草中表达苜蓿花叶病毒和烟草坏死病毒的外壳蛋白基因
C M van Dun,J F Bol,L Van Vloten-Doting
C M van Dun
Using the Agrobacterium tumefaciens binary vector system, a chimeric gene consisting of the cauliflower mosaic virus 35 S promoter, alfalfa mosaic virus (AIMV) coat protein (CP) cistron, and the nopaline synthase polyadenylation signal was ...
Biological activity of T7 transcripts of a prototype clone and a sequence variant clone of a satellite RNA of cucumber mosaic virus [0.03%]
黄瓜花叶病毒卫星RNA原型克隆和序列变异体克隆T7转录本的生物学活性
G Kurath,P Palukaitis
G Kurath
Molecular cloning of the (D)CARNA 5 (previously known as n-CARNA 5) necrosis-inducing satellite RNA of cucumber mosaic virus produced a prototype clone (pDsat4) and a sequence variant clone (pDsat1). pDsat1 contained 10 nucleotide changes b...
The spherically averaged structure of a DNA isometric plant virus: cauliflower mosaic virus [0.03%]
球均化的DNA等边植物病毒的结构:花椰菜马osaic病毒
J Kruse,P Timmins,J Witz
J Kruse
The organization of cauliflower mosaic virus was reinvestigated by neutron small-angle scattering in buffers containing various amounts of D2O. The molecular weight of the virions and their DNA content were determined and found to agree wel...
In vitro translation products of mrnas derived from TMV-infected tobacco exhibiting a hypersensitive response [0.03%]
TMV感染后引起HR的烟草叶片中与HR有关的mRNA及其表达产物的研究
T E Smart,D D Dunigan,M Zaitlin
T E Smart
Expression of the hypersensitive response (HSR) to tobacco mosaic virus (TMV) infection in Nicotiana tabacum L. cv. Xanthi-nc (genotype NN) is controlled by the single dominant "N" gene and is temperature sensitive. TMV-infected Xanthi-nc t...
Nucleotide sequence analysis of six satellite RNAs of cucumber mosaic virus: primary sequence and secondary structure alterations do not correlate with differences in pathogenicity [0.03%]
黄瓜花叶病毒六种卫星RNA的核苷酸序列分析:初级序列和二级结构的变化与致病性差异无关
F García-Arenal,M Zaitlin,P Palukaitis
F García-Arenal
The nucleotide sequences of six satellite RNAs of cucumber mosaic virus (CMV) differing in their pathological properties have been determined. Although these RNAs varied in length from 333 to 342 nucleotides, the extent of nucleotide sequen...