Structural characteristics correlate with immune responses induced by HIV envelope glycoprotein vaccines [0.03%]
结构特征与HIV衣壳糖蛋白疫苗诱导的免疫反应相关性研究
Victoria A Sharma,Elaine Kan,Yide Sun et al.
Victoria A Sharma et al.
HIV envelope glycoprotein (Env) is the target for inducing neutralizing antibodies. Env is present on the virus surface as a trimer, and, upon binding to CD4, a cascade of events leads to structural rearrangement exposing the co-receptor bi...
DNA nucleotide sequence analysis of the PvuII DNA fragment L of the genome of insect iridescent virus type 6 reveals a complex cluster of multiple tandem, overlapping, and interdigitated repetitive DNA elements [0.03%]
PvuⅡ限制性内切酶切割的昆虫虹彩病毒6型基因组DNA片段L的核苷酸序列分析表明它包含复杂的多重复、重叠和镶嵌排列的重复序列簇
M Fischer,P Schnitzler,J R Scholz et al.
M Fischer et al.
The DNA nucleotide sequence of the PvuII DNA fragment L (0.920 to 0.944 map units (m.u.)) of the genome (209 kbp) of insect iridescent virus type 6 was determined. The size of this DNA fragment was 5064 by with a base composition of 39.79% ...
Organization of the adeno-associated virus (AAV) capsid gene: mapping of a minor spliced mRNA coding for virus capsid protein [0.03%]
腺伴随病毒(AAV)衣壳基因的组织定位：一个编码病毒衣壳蛋白的次要剪辑mRNA的测定
P Cassinotti,M Weitzand,J D Tratschin
P Cassinotti
We have mapped a minor spliced transcript with mRNA properties which is derived from the capsid gene promoter (P40) of the adeno-associated virus (AAV) genome. By S1 nuclease mapping as well as primer extension analysis this mRNA was found ...
SV(LM21), a mutant of Sindbis virus able to grow in Aedes albopictus cells in the absence of methionine, shows increased sensitivity to S-adenosylhomocysteine hydrolase inhibitors such as neplanocin A [0.03%]
SV(LM21)，是一种辛德毕斯病毒突变株，能够在不含蛋氨酸的条件下于白纹伊蚊细胞中增殖，并且对S-腺苷蛋氨酸消旋酶抑制剂（如neplanocin A）敏感性增加
R K Durbin,V Stollar,E D Clercq
R K Durbin
Inhibition of S-adenosylhomocysteine (AdoHcy) hydrolase by compounds such as Neplanocin A (NPA) leads to the build-up of AdoHcy and the inhibition of methyltransferase enzymes. Whether assayed by efficiency of plaquing or virus yield, SVLM2...
Non-structural proteins and RNAs of alfalfa mosaic virus synthesized in tobacco and cowpea protoplasts [0.03%]
在烟草和菜豆原生质体中合成的苜蓿花叶病毒非结构蛋白和RNA
H van Pelt-Heerschap,H Verbeek,M J Huisman et al.
H van Pelt-Heerschap et al.
Three proteins, reacting specifically with sera raised against synthetic peptides identical to C-terminal amino acid sequences in alfalfa mosaic virus (AIMV) proteins P1, P2, and P3 translated in vitro from the AIMV RNAs 1, 2, and 3, respec...
Cauliflower mosaic virus replication intermediates are encapsidated into virion-like particles [0.03%]
cauliflower mosaic病毒复制中间体以病毒颗粒样粒子的形式封装起来
L E Marsh,T J Guilfoyle
L E Marsh
We have identified putative cauliflower mosaic virus (CaMV) replication intermediates of first (minus)-strand DNA synthesis which are associated with particles having properties similar to virions. The virion-like particles containing the p...
Protein encoded by ORF I of cauliflower mosaic virus is part of the viral inclusion body [0.03%]
芜菁花叶病毒ORF I表达蛋白与病毒包涵体相关
J A Martinez-Izquierdo,J Fütterer,T Hohn
J A Martinez-Izquierdo
Coding sequences of ORF I from cauliflower mosaic virus were cloned in an Escherichia coli expression vector. A protein derived from this ORF was used to raise antibodies in rabbits. Immunoblots revealed that in infected plants the ORF I pr...
Tobacco mosaic virus particles uncoat and express their RNA in Xenopus laevis oocytes: implications for early interactions between plant cells and viruses [0.03%]
烟草花叶病毒颗粒在非洲爪蟾卵母细胞中解衣脱壳并表达其RNA：对于植物细胞与病毒早期相互作用的启示
P C Turner,P A Watkins,M Zaitlin et al.
P C Turner et al.
Xenopus laevis oocytes were microinjected with tobacco mosaic virus (TMV) particles or unencapsidated TMV RNA at equivalent RNA concentrations. After a 5-hr incubation, batches of 10 oocytes from each treatment were frozen and lysed, and th...
Physical mapping of the DNA genome of insect iridescent virus type 9 from Wiseana spp. larvae [0.03%]
昆虫虹彩病毒9型的物理图谱测绘摘要来自Wiseana属幼虫的DNA基因组
V K Ward,J Kalmakoff
V K Ward
A physical map for the DNA genome of insect iridescent virus type 9 isolated from Wiseana spp. larvae [Lepidoptera: Hepialidae] was constructed using the restriction enzymes BamHl, EcoRl, and Pstl. Viral DNA was cloned into the plasmids pBR...
Immunocytochemical localization of the 130K and 180K proteins (putative replicase components) of tobacco mosaic virus [0.03%]
烟草花叶病毒的130K和180K蛋白（假定的复制酶组分）的免疫细胞化学定位
T Saito,D Hosokawa,T Meshi et al.
T Saito et al.
We have revealed the cellular localization of the putative replicase components of tobacco mosaic virus (TMV), 130K and 180K proteins, in TMV-infected tobacco leaves by the immunogold technique with antisera which specifically react with th...