Site-specific cleavage and religation of viral RNAs. I. Infectivity of barley stripe mosaic virus RNA religated from functionally active segments and restoration of the internal poly(A) tract in progeny
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The genomic RNAs of barley stripe mosaic virus (BSMV) were used in experiments involving site-specific cleavage to yield noninfective but functionally active fragments and subsequent restoration of infectious viral RNA by means of religation. The specific frag... ...