Acyclic Cucurbit[n]uril-Type Containers as Receptors for Neuromuscular Blocking Agents: Structure-Binding Affinity Relationships [0.03%]
神经肌肉阻断剂的受体无环葫芦[n]脲型容器：结构-结合亲和力关系
David Shaya,Lyle Isaacs
David Shaya
Acyclic cucurbit[n]uril molecular containers 1 and 2C3 have previously been shown to strongly bind to the neuromuscular blocking agents rocuronium, vecuronium, pancuronium, and cisatracurium in vitro by optical methods and to reverse neurom...
Pyrrolysyl-tRNA synthetase, an aminoacyl-tRNA synthetase for genetic code expansion [0.03%]
蛋白酶体驻留因子 Psr1/Psr2 通过介导 Retinitis Pigmentosa 27 相关的视锥细胞特异性蛋白质降解来调控视力
Ana Crnković,Tateki Suzuki,Dieter Söll et al.
Ana Crnković et al.
Genetic code expansion (GCE) has become a central topic of synthetic biology. GCE relies on engineered aminoacyl-tRNA synthetases (aaRSs) and a cognate tRNA species to allow codon reassignment by co-translational insertion of non-canonical ...
Lack of discrimination against non-proteinogenic amino acid norvaline by elongation factor Tu from Escherichia coli [0.03%]
大肠杆菌elongation因子Tu对非蛋白氨基酸norvaline缺乏识别性
Nevena Cvetesic,Irena Akmacic,Ita Gruic-Sovulj
Nevena Cvetesic
The GTP-bound form of elongation factor Tu (EF-Tu) brings aminoacylated tRNAs (aa-tRNA) to the A-site of the ribosome. EF-Tu binds all cognate elongator aa-tRNAs with highly similar affinities, and its weaker or tighter binding of misacylat...
Competitive Adsorption of Three Human Plasma Proteins onto Sulfhydryl-to-sulfonate Gradient Surfaces [0.03%]
三种人血清蛋白在硫醇-磺酸根梯度表面的竞争吸附
Yong-Xue Ding,Vladimir Hlady
Yong-Xue Ding
Competitive adsorption of three human plasma proteins: albumin (HSA), fibrinogen (Fgn), and immunoglobulin G (IgG) from their ternary solution mixtures onto a sulfhydryl-to-sulfonate gradient surface was investigated using spatially-resolve...
Single Molecule Probing of Exocytotic Protein Interactions Using Force Spectroscopy [0.03%]
使用力谱学对囊泡释放蛋白进行单分子检测
Vedrana Montana,Wei Liu,Umar Mohideen et al.
Vedrana Montana et al.
Relatively recently, the Atomic Force Microscope (AFM) emerged as a powerful tool for single molecule nanomechanical investigations. Parameters that can be measured by force spectroscopy using AFM, such as the force and total mechanical ext...
How Surface Heterogeneity Affects Protein Adsorption: Annealing of OTS Patterns and Albumin Adsorption Kinetics [0.03%]
OTS图案的退火处理及白蛋白吸附动力学对表面异质性影响的研究
Gerald N Hodgkinson,Vladimir Hlady
Gerald N Hodgkinson
Fluorescence microscopy and intensity histogram analysis techniques were used to monitor spatially-resolved albumin adsorption kinetics to model heterogeneous surfaces on sub-μm scales. Several distinct protein subpopulations were resolved...