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Pyrrolysyl-tRNA synthetase, an aminoacyl-tRNA synthetase for genetic code expansion

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Genetic code expansion (GCE) has become a central topic of synthetic biology. GCE relies on engineered aminoacyl-tRNA synthetases (aaRSs) and a cognate tRNA species to allow codon reassignment by co-translational insertion of non-canonical amino acids (ncAAs) ... ...