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Analytical chemistry. 2012 Dec 18;84(24):10845-51. doi: 10.1021/ac303095z Q16.72025

Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification

一种快速无标记监测方法在 exonuclease Ⅲ参与的目标循环放大中的应用研究 翻译改进

Qinfeng Xu  1, Anping Cao, Ling-fei Zhang, Chun-yang Zhang

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  • 1 Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
  • DOI: 10.1021/ac303095z PMID: 23167838

    摘要 Ai翻译

    Target recycling-oriented amplification has been widely applied for sensitive detection of DNA, RNA, and proteins due to its successful overcoming the inherent limitation of target-to-signal ratio of 1:1 in the traditional hybridization assay. Exonuclease III (Exo III) is usually used as the cleavage enzyme in the target recycling-oriented amplification because of its easy availability, high catalytic activity, and wide applicability. Even though Exo III i... ...点击完成人机验证后继续浏览
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    期刊名:Analytical chemistry

    缩写:ANAL CHEM

    ISSN:0003-2700

    e-ISSN:1520-6882

    IF/分区:6.7/Q1

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    Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification