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Analytica chimica acta. 2020 Sep 22:1131:1-8. doi: 10.1016/j.aca.2020.07.025 Q16.02025

Detection of microRNAs by the combination of Exonuclease-III assisted target recycling amplification and repeated-fishing strategy

外切酶-III辅助靶标循环放大与重复富集策略结合检测microRNAs 翻译改进

Xiao-Mei Yan  1, Yin-Qi Wang  1, Yao Chen  2, Zeng-Ping Chen  3, Ru-Qin Yu  1

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作者单位

  • 1 State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, PR China.
  • 2 Hunan Key Lab of Biomedical Materials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou, 412008, PR China. Electronic address: chenyao717@hnu.edu.cn.
  • 3 State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, PR China. Electronic address: zpchen@hnu.edu.cn.

    • DOI: 10.1016/j.aca.2020.07.025 PMID: 32928469

    摘要 Ai翻译

    A simple but effective method for the detection of miRNAs was proposed by integrating exonuclease-III assisted target recycling amplification and repeated-fishing strategy. In the proposed method, exonuclease-III assisted target recycling amplification reaction is adopted to produce a large amount of DNA fragments with fluorescence group at its 5' end in the presence of the target miRNA, which are then repeatedly fished out from the reaction mixture by a g... ...点击完成人机验证后继续浏览
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    期刊名：Analytica chimica acta

    缩写：ANAL CHIM ACTA

    ISSN：0003-2670

    e-ISSN：1873-4324

    IF/分区：6.0/Q1

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    Detection of microRNAs by the combination of Exonuclease-III assisted target recycling amplification and repeated-fishing strategy