Divergent roles of Hsp70 chaperones in orthoflavivirus protein secretion and virion formation [0.03%]
Hsp70分子伴侣在正链 flavivirus 蛋白分泌和病毒颗粒形成中的不同作用
Lea Blank,Christin Lorenz,Imke Steffen
Lea Blank
Orthoflaviviruses, such as tick-borne encephalitis virus (TBEV) and West Nile virus (WNV), can cause severe neurological disease and remain without specific antiviral treatments. We found that orthoflavivirus envelope (E) and non-structural...
Mechanistic insights into the impact of prenatal viral infections on maternal and offspring immunity [0.03%]
孕期病毒感染对母体和子代免疫影响的机制研究新进展
Gielenny M Salem,Tamiris Azamor,Débora Familiar-Macedo et al.
Gielenny M Salem et al.
Global outbreaks of human immunodeficiency virus (HIV) and respiratory viruses - severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza, accounted for ~50 million infections in 2024. Prenatal exposure to these viruses po...
The soluble G protein of respiratory syncytial virus promotes viral dissemination via TLR2-mediated NLRP3 priming and pyroptosis [0.03%]
呼吸道合胞病毒的可溶性G蛋白通过TLR2介导的NLRP3原发诱导和pyroptosis促进病毒扩散
Robert Meineke,Ayse Agac,Marie-Christin Knittler et al.
Robert Meineke et al.
Respiratory syncytial virus (RSV) causes severe lower respiratory tract infections in infants, immunocompromised individuals, and older adults. Although vaccines and monoclonal antibodies have recently become available, understanding RSV pa...
Efficient whole-genome sequencing of Monkeypox virus using a novel nuclease-multiple displacement amplification enrichment method [0.03%]
一种新型核酸酶多次分散复制富集法对猴痘病毒全基因组高效测序
Masayasu Misu,Takeshi Kurosu,Tomoki Yoshikawa et al.
Masayasu Misu et al.
Monkeypox virus (MPXV) has a large double-stranded DNA genome (~200 kb), which presents challenges for whole-genome sequencing. Conventional enrichment methods have specific limitations. To address them, we developed a novel enrichment stra...
In vitro synthesis of active recombinant orthoflavivirus nonstructural polyproteins in detergent micelles for biochemical analysis [0.03%]
用于生化分析的二毛病毒非结构聚蛋白在去垢剂胶束中的体外合成
Hirotaka Takahashi,Yusuke Uchiage,Yuki Emura et al.
Hirotaka Takahashi et al.
The nonstructural (NS) proteins of orthoflaviviruses are cleaved from a precursor polyprotein by both host and viral proteases, subsequently forming a replication complex. However, the biochemical properties underlying polyprotein cleavage ...
Characterisation of the tilapia lake virus proteome and identification of an 11th protein, S9-F3 [0.03%]
鰈湖病毒蛋白组的表征及第11种蛋白S9-F3的鉴定
Nunticha Pankaew,Dominic Kurian,Federico De Angelis et al.
Nunticha Pankaew et al.
Tilapia Lake Virus (TiLV) is an emerging RNA virus threatening global tilapia aquaculture. While proteins encoded by segments 1-3 (polymerase) and 4 (nucleoprotein) are characterised, the functions of polypeptides from the remaining segment...
K5 polysaccharides inhibit SARS-CoV-2 infection by preventing spike-proteolytic priming [0.03%]
K5多糖通过阻止刺突蛋白酶原激活抑制SARS-CoV-2感染
Maria Milanesi,Chiara Urbinati,Liv Zimmermann et al.
Maria Milanesi et al.
SARS-CoV-2 spike glycoprotein is a promising drug target due to its crucial role in viral infection. Heparin, a long linear polysaccharide that inhibits SARS-CoV-2 infection by acting on spike, has limited antiviral applications due to its ...
Visualising viral interactions and mechanisms at the nanoscale with expansion microscopy [0.03%]
利用膨胀显微镜在纳米尺度上可视化病毒相互作用及工作机制
Lisa Övermöhle,Maximilian Baum,Rohan Bhatia et al.
Lisa Övermöhle et al.
Expansion microscopy is a groundbreaking technique that enables nanoscale imaging of biological specimens using standard optical microscopes. By embedding specimens in swellable hydrogels, it achieves sub-diffraction resolution. Compatible ...
Discovery of potent cross-neutralizing antibodies against Marburg and Ravn viruses with therapeutic potential [0.03%]
具有治疗潜力的马堡病毒和拉文病毒强效交叉中和抗体的发现
Takeshi Saito,Hiroko Miyamoto,Manabu Igarashi et al.
Takeshi Saito et al.
Marburg virus (MARV) and Ravn virus (RAVV) are highly pathogenic filoviruses that cause Marburg virus disease (MVD), for which no approved treatment currently exists. In this study, we describe a panel of 10 novel mouse-derived monoclonal a...
VHH antibody loop guides design of a synthetic macrocyclic peptide that potently blocks influenza virus membrane fusion [0.03%]
VHH抗体可变环引导设计合成的大环肽以有效阻断流感病毒膜融合
Rameshwar U Kadam,Jarek Juraszek,Boerries Brandenburg et al.
Rameshwar U Kadam et al.
Miniaturizing biologically complex structural motifs to produce synthetic functional mimetics holds significant promise for development of new therapeutic modalities. Here, we demonstrate a unique approach using the key binding loop of the ...