Development of SYK NanoBRET cellular target engagement assays for gain-of-function variants [0.03%]
SYK NanoBRET细胞靶点参与测定的功效变异体的发展
Jacob L Capener,James D Vasta,Vittorio L Katis et al.
Jacob L Capener et al.
Spleen tyrosine kinase (SYK) is a non-receptor tyrosine kinase that is activated by phosphorylation events downstream of FcR, B-cell and T-cell receptors, integrins, and C-type lectin receptors. When the tandem Src homology 2 (SH2) domains ...
Cysteine-rich zinc finger proteins and the nuclear factor kappa-B pathway [0.03%]
半胱氨酸丰富型锌指蛋白和核因子κB途径
Andrew T Stoltzfus,Sarah L J Michel
Andrew T Stoltzfus
Inflammation-related disorders, such as autoimmune diseases and cancer, impose a significant global health burden. Zinc finger proteins (ZFs) are ubiquitous metalloproteins which regulate inflammation and many biological signaling pathways ...
Molecular interactions at the interface: polyoxometalates of the Anderson-Evans type and lipid membranes [0.03%]
安德森-埃文斯型多金属氧酸盐与脂质膜的界面分子相互作用
Alina A Pashkovskaya,Nadiia I Gumerova,Annette Rompel et al.
Alina A Pashkovskaya et al.
Polyoxometalates (POMs) are metal-oxygen clusters composed of {MO6} octahedra that have attracted considerable attention due to their remarkable antiviral, antibacterial and antitumor activities. Despite their potential, the molecular mecha...
Ananya A Basu,Xiaoyu Zhang
Ananya A Basu
The development of multiplexing technologies for proteomics has enabled the quantification of proteins on a global scale across samples with high confidence. In the covalent ligand discovery pipeline, quantitative proteomics can be used to ...
Structural and kinetic characterization of DUSP5 with a Di-phosphorylated tripeptide substrate from the ERK activation loop [0.03%]
人源DUSP5与含双磷酸化残基的ERK激活环肽段的结构及动力学研究
Andrea Imhoff,Noreena L Sweeney,Robert D Bongard et al.
Andrea Imhoff et al.
Introduction: Dual specific phosphatases (DUSPs) are mitogen-activated protein kinase (MAPK) regulators, which also serve as drug targets for treating various vascular diseases. Previously, we have presented mechanistic c...
An activity-based probe for antimicrobial target DXP synthase, a thiamin diphosphate-dependent enzyme [0.03%]
一种针对依赖焦磷酸硫胺素的抗菌靶标DXP合酶的基于活性的小分子探针
Lauren B Coco,Caren L Freel Meyers
Lauren B Coco
This work reports an alkyl acetylphosphonate (alkylAP) activity-based probe (ABP) for 1-deoxy-d-xylulose 5-phosphate synthase DXPS, a promising antimicrobial target. This essential thiamin diphosphate (ThDP)-dependent enzyme operates at a b...
An in vivo BSL-2 model for henipavirus infection based on bioluminescence imaging of recombinant Cedar virus replication in mice [0.03%]
基于重组红杉病毒小鼠体内复制的生物发光成像建立活体亨尼帕病毒属感染BSL-2模型
Celeste Huaman,Caitlyn Clouse,Madeline Rader et al.
Celeste Huaman et al.
Henipaviruses are enveloped single-stranded, negative-sense RNA viruses of the paramyxovirus family. Two henipaviruses, Nipah virus and Hendra virus, cause a systemic respiratory and/or neurological disease in humans and ten additional spec...
Quantitative Measurement of Transthyretin Mistargeting by Proximity Labeling and Parallel Reaction Monitoring [0.03%]
通过邻近标记和并行反应监测进行运甲状腺素蛋白误定位的定量检测
Ziqi Lyu,Joseph C Genereux
Ziqi Lyu
Proximity labeling is a powerful approach for characterizing subcellular proteomes. We recently demonstrated that proximity labeling can be used to identify mistrafficking of secretory proteins, such as occurs during pre-emptive quality con...