Developing a microfluidic-based epicPCR reveals diverse potential hosts of the mcrA gene in marine cold seep [0.03%]
基于微流控的epicPCR技术揭示了mcrA基因在海洋冷泉中多样化的潜在宿主
Wenli Shen,Danrui Wang,Jiangtao Li et al.
Wenli Shen et al.
Anaerobic methanotrophic (ANME) microbes play a crucial role in the bioprocess of anaerobic oxidation of methane (AOM). However, due to their unculturable status, their diversity is poorly understood. In this study, we established a microfl...
The endocytic pathway for absorption of exogenous RNAs in Verticillium dahliae [0.03%]
大丽轮枝菌吸收外源RNAs的胞内吞途径
Chuanhui Liu,Chen Cui,Guanyin Zhou et al.
Chuanhui Liu et al.
RNAi technologies have been exploited to control viruses, pests, oomycetes, and fungal phytopathogens that cause disasters in host plants, including many agronomically significant crops. Double-stranded RNA (dsRNA) or small interfering RNA ...
A new type of ArsR transcriptional repressor controls transcription of the arsenic resistance operon of Arsenicibacter rosenii SM-1 [0.03%]
一种新的ArsR转录阻遏蛋白控制砷抗性操纵子Arsenicaibacter rosenii SM-1的转录
Yujie Zhang,Wenjun Wu,Ke Huang et al.
Yujie Zhang et al.
Arsenic is the most common toxic metalloid in the environment. Nearly all organisms have genes for arsenic detoxification. Arsenic detoxification genes are frequently organized in chromosomal or plasmid-encoded arsenic resistance (ars) oper...
Avian tuberculosis identified as the potential disease in an outbreak in wild migratory birds in China [0.03%]
中国野鸟迁徙过程中暴发潜在疾病的病原体为鸟类结核菌
Chunge Zhang,Liang Wang,Cheng Zhang et al.
Chunge Zhang et al.
This study identifies avian tuberculosis as a potential cause of mass mortality in wild migratory birds in Inner Mongolia, China. Combining meta-transcriptomic sequencing and histopathological analysis, it reveals one of the rare instances ...
NAC4ED: A high-throughput computational platform for the rational design of enzyme activity and substrate selectivity [0.03%]
基于理性设计的高效催化剂计算平台用于提升酶活性及选择性
Chuanxi Zhang,Yinghui Feng,Yiting Zhu et al.
Chuanxi Zhang et al.
In silico computational methods have been widely utilized to study enzyme catalytic mechanisms and design enzyme performance, including molecular docking, molecular dynamics, quantum mechanics, and multiscale QM/MM approaches. However, the ...
Bingxin Zhou,Yang Tan,Yutong Hu et al.
Bingxin Zhou et al.
Advances in deep learning have significantly aided protein engineering in addressing challenges in industrial production, healthcare, and environmental sustainability. This review frames frequently researched problems in protein understandi...
Genotype-microbiome-metabolome associations in early childhood and their link to BMI [0.03%]
儿童遗传基因-微生物-代谢组关联及其与BMI的联系
Andrea Aparicio,Zheng Sun,Diane R Gold et al.
Andrea Aparicio et al.
Through the analysis of data from children aged 6 months to 8 years enrolled in the Vitamin D Antenatal Asthma Reduction Trial (VDAART), significant simultaneous associations were identified between variants in the fragile histidine triad (...
Measurement of optical density of microbes by multi-light path transmission method [0.03%]
多光路透射法测量微生物的光学浓度
Hongwei Wang,Carina M Gu,Sujuan Xu et al.
Hongwei Wang et al.
Optical density (OD) is an important indicator of microbial density, and a commonly used variable in growth curves to express the growth of microbial culture. However, OD values show a linear relationship with bacterial concentration only a...
Phospholipase PlcH is involved in the secretion of cell wall glycoproteins and contributes to the host immune response of Aspergillus fumigatus [0.03%]
磷脂酶PlcH参与烟曲霉细胞壁糖蛋白的分泌并影响宿主免疫应答
Jinbin Hao,Yin Guo,Hui Zhou et al.
Jinbin Hao et al.
Glycosylphosphatidylinositol (GPI) anchoring is one of the conserved posttranslational modifications in eukaryotes that attach proteins to the plasma membrane. In fungi, in addition to plasma membrane GPI-anchored proteins (GPI-APs), some G...
HPClas: A data-driven approach for identifying halophilic proteins based on catBoost [0.03%]
基于catBoost的鉴定极端嗜盐蛋白的数据驱动方法
Shantong Hu,Xiaoyu Wang,Zhikang Wang et al.
Shantong Hu et al.
Halophilic proteins possess unique structural properties and show high stability under extreme conditions. This distinct characteristic makes them invaluable for application in various aspects such as bioenergy, pharmaceuticals, environment...