A CRISPR-nonhomologous end-joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus [0.03%]
基于CRISPR非同源末端连接的快速高效基因敲除策略在脓肿分枝杆菌中的应用
Sanshan Zeng,Yanan Ju,Md Shah Alam et al.
Sanshan Zeng et al.
Mycobacterium abscessus, a fast-growing, non-tuberculous mycobacterium resistant to most antimicrobial drugs, causes a wide range of serious infections in humans, posing a significant public health challenge. The development of effective ge...
Cpx-mediated amino acid sensing diversifies gastrointestinal colonization of Klebsiella pneumoniae [0.03%]
Cpx介导的氨基酸感应可使肺炎克雷伯菌肠道定植多样化
Danyang Li,Qiucheng Shi,Liuqing He et al.
Danyang Li et al.
Klebsiella pneumoniae is a Gram-negative opportunistic pathogenic bacterium that occasionally inhabits the human gastrointestinal tracts. Gut-colonized K. pneumoniae may then metastasize to other organs and tissues, thus causing severe infe...
Correction to "NAC4ED: A high-throughput computational platform for the rational design of enzyme activity and substrate selectivity" [0.03%]
对“NAC4ED：一个用于理性设计酶活性和底物选择性的高通量计算平台”的勘误
[This corrects the article DOI: 10.1002/mlf2.12154.]. © 2025 The Author(s). mLife published by John Wiley & Sons Australia, Ltd on behalf of Institute o...
Published Erratum
mLife. 2025 Mar 27;4(2):226. DOI:10.1002/mlf2.70000 2025
Archaea show different geographical distribution patterns compared to bacteria and fungi in Arctic marine sediments [0.03%]
古菌与北极海洋沉积物中的细菌和真菌具有不同的地理分布模式
Jianxing Sun,Hongbo Zhou,Haina Cheng et al.
Jianxing Sun et al.
Microorganisms dominate marine environments in the polar oceans and are known to harbor greater diversity and abundance than was once thought, and yet, little is known about their biogeographic distribution patterns in marine sediments at a...
Identification of novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A [0.03%]
热硫珠菌REY15A中Ced和Ups系统新组分的识别
Pengju Wu,Mengqi Zhang,Yanlu Kou et al.
Pengju Wu et al.
In Sulfolobales cells, transcription of the Ups (UV-inducible pili of Sulfolobus) and Ced (Crenarchaeal system for exchange of DNA) genes is highly induced by DNA damage, and the two systems play key roles in pili-mediated cell aggregation ...
Jingchao Zhang,Yan Luo,Yiwu Zong et al.
Jingchao Zhang et al.
In Pseudomonas aeruginosa, the dynamic activity of type IV pilus (TFP) is essential for various bacterial behaviors. While PilU is considered a homolog of the TFP disassembling motor PilT, its specific roles remain unclear. Using pilus visu...
Complexation of CcmB with CcmACD safeguards heme translocation for cytochrome c maturation [0.03%]
CcmB与CcmACD的复合确保了血红素转运以促进细胞色素c的成熟过程
Yuanyou Xu,Wei Wang,Qianrou Zhang et al.
Yuanyou Xu et al.
Cytochrome c maturation (CCM), a posttranslational modification involving covalent attachment of heme to polypeptides (apocyt c), is essential for the activity and cellular function of cytochromes c. Here, we identify and substantiate CcmB ...
Reviewer Acknowledgments [0.03%]
审稿人鸣谢
Editorial
mLife. 2025 Jan 28;4(1):104-105. DOI:10.1002/mlf2.12162 2025
Genome-wide siRNA library screening identifies human host factors that influence the replication of the highly pathogenic H5N1 influenza virus [0.03%]
基因组范围的siRNA文库筛选确定影响高致病性H5N1流感病毒复制的人宿主因子
Guangwen Wang,Li Jiang,Jinliang Wang et al.
Guangwen Wang et al.
The global dissemination of H5 avian influenza viruses represents a significant threat to both human and animal health. In this study, we conducted a genome-wide siRNA library screening against the highly pathogenic H5N1 influenza virus, le...
Synthetic evolution of Saccharomyces cerevisiae for biomanufacturing: Approaches and applications [0.03%]
用于生物制造的毕赤酵母菌的合成进化：方法与应用
Zhen Wang,Xianni Qi,Xinru Ren et al.
Zhen Wang et al.
The yeast Saccharomyces cerevisiae is a well-studied unicellular eukaryote with a significant role in the biomanufacturing of natural products, biofuels, and bulk and value-added chemicals, as well as the principal model eukaryotic organism...