Cultivating the uncultured: Harnessing the "sandwich agar plate" approach to isolate heme-dependent bacteria from marine sediment [0.03%]
利用“夹层培养基平板”方法从海洋沉积物中分离依赖血红素的细菌
Jing Zhang,Qi-Yun Liang,Da-Shuai Mu et al.
Jing Zhang et al.
In the classical microbial isolation technique, the isolation process inevitably destroys all microbial interactions and thus makes it difficult to culture the many microorganisms that rely on these interactions for survival. In this study,...
RsaL is a self-regulatory switch that controls alternative biosynthesis of two AHL-type quorum sensing signals in Pseudomonas aeruginosa PA1201 [0.03%]
PA1201中RSA L作为自我调控开关控制两种AHL型群体感应信号的交替产生
Ya-Wen He,Zi-Jing Jin,Ying Cui et al.
Ya-Wen He et al.
Pseudomonas aeruginosa is a ubiquitous and metabolically versatile microorganism naturally found in soil and water. It is also an opportunistic pathogen in plants, insects, animals, and humans. In response to increasing cell density, P. aer...
Cong Liu,Rui Shi,Marcus S Jensen et al.
Cong Liu et al.
Nucleotide second messengers are highly versatile signaling molecules that regulate a variety of key biological processes in bacteria. The best-studied examples are cyclic AMP (cAMP) and bis-(3'-5')-cyclic dimeric guanosine monophosphate (c...
Annan Ming,Jianxin Zhao,Yihan Liu et al.
Annan Ming et al.
O-glycosylation is an ancient yet underappreciated protein posttranslational modification, on which many bacteria and viruses heavily rely to perform critical biological functions involved in numerous infectious diseases or even cancer. But...
William B Whitman,Maria Chuvochina,Brian P Hedlund et al.
William B Whitman et al.
The SeqCode, formally called the Code of Nomenclature of Prokaryotes Described from Sequence Data, is a new code of nomenclature in which genome sequences are the nomenclatural types for the names of prokaryotic species. While similar to th...
Insertion sequence transposition activates antimycobacteriophage immunity through an lsr2-silenced lipid metabolism gene island [0.03%]
插入序列转座通过lsr2沉默的脂质代谢基因岛激活抗分枝杆菌噬菌体免疫
Yakun Li,Yuyun Wei,Xiao Guo et al.
Yakun Li et al.
Insertion sequences (ISs) exist widely in bacterial genomes, but their roles in the evolution of bacterial antiphage defense remain to be clarified. Here, we report that, under the pressure of phage infection, the IS1096 transposition of My...
Dawn E Holmes,Trevor L Woodard,Jessica A Smith et al.
Dawn E Holmes et al.
Anaerobic microbial corrosion of iron-containing metals causes extensive economic damage. Some microbes are capable of direct metal-to-microbe electron transfer (electrobiocorrosion), but the prevalence of electrobiocorrosion among diverse ...
Understanding the rapid spread of antimicrobial resistance genes mediated by IS 26 [0.03%]
了解IS 26介导的耐药基因快速传播机制
Na Tang,Dawei Wei,Yuan Zeng et al.
Na Tang et al.
Insertion sequences (ISs) promote the transmission of antimicrobial resistance genes (ARGs) across bacterial populations. However, their contributions and dynamics during the transmission of resistance remain unclear. In this study, we sele...
Ji Zeng,Shuying Fang,Jinquan Guo et al.
Ji Zeng et al.
Clostridioides difficile is a leading cause of healthcare-associated infections, causing billions of economic losses every year. Its symptoms range from mild diarrhea to life-threatening damage to the colon. Transmission and recurrence of C...
Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus [0.03%]
不受限制的pRN1衍生设计及其在构建Saccharolobus islandicus双质粒载体系统中的应用
Pengpeng Zhao,Xiaonan Bi,Xiaoning Wang et al.
Pengpeng Zhao et al.
Saccharolobus islandicus REY15A represents one of the very few archaeal models with versatile genetic tools, which include efficient genome editing, gene silencing, and robust protein expression systems. However, plasmid vectors constructed...