High-speed in vivo calcium recording using structured illumination with self-supervised denoising [0.03%]
基于自监督去噪的结构照明在体高速钙成像
Forest Speed,Catherine A Saladrigas,Alec Teel et al.
Forest Speed et al.
High-speed widefield fluorescence imaging of neural activity in vivo is fundamentally limited by fluctuations in recorded signal due to background contamination and stochastic noise. In this study, we show background and shot noise-reduced ...
Gastón A Ayubi,Bartlomiej Kowalski,Alfredo Dubra
Gastón A Ayubi
The normalized cross-correlation ( N C C ) is widely used for image registration due to its simple geometrical interpretation and being feature-agnostic. Here, after reviewing N C C definitions for images with an arbitrary number of dimensi...
Simple, cost-effective, highly stable solid-state photoluminescence standard for fluorometer calibration in low-resource settings [0.03%]
简单低成本高稳定的固态光致发光标准用于资源匮乏地区荧光计校准
Joshua Eger,Mark Bailly,Jennifer Blain Christen
Joshua Eger
The proposed photoluminescence calibration standard comprises a solid-state phosphor film, neutral density gel filter(s), and a 3D-printed optomechanical cartridge. The proposed standard demonstrated exceptional photostability; photolumines...
Photothermal optical coherence tomography for 3D live cell detection and mapping [0.03%]
光热光学相干层析法用于活细胞的三维检测与成像
Jingyu Sun,Tianqi Fang,Hongjun Wang et al.
Jingyu Sun et al.
Imaging cells in their 3D environment with molecular specificity is important to cell biology study. Widely used microscopy techniques, such as confocal microscopy, have limited imaging depth when probing cells in optically scattering media...
Complex optically computed phase microscopy for label-free sub-cellular imaging [0.03%]
复杂光学计算相位显微镜实现无标记亚细胞成像
Xuan Liu,Yuwei Liu,Shupei Yu et al.
Xuan Liu et al.
In this study, we investigated a complex optically computed phase microscopy (complex-OCPM) technology. Based on a low coherence interferometer and an innovative optical computation approach, the complex-OCPM imaging system achieves depth r...
Computational microscopy for fast widefield deep-tissue fluorescence imaging using a commercial dual-cannula probe [0.03%]
基于商用双管探针的快速场宽深层荧光显微成像计算显微学技术
Ekata Mitra,Ruipeng Guo,Soren Nelson et al.
Ekata Mitra et al.
A solid-glass cannula serves as a micro-endoscope that can deliver excitation light deep inside tissue while also collecting emitted fluorescence. Then, we utilize deep neural networks to reconstruct images from the collected intensity dist...