Cobalamin-Dependent Radical S-Adenosylmethionine Enzymes: Capitalizing on Old Motifs for New Functions [0.03%]
钴胺素依赖性游离基S-腺苷甲硫氨酸酶：利用旧有模式产生新的功能
Jennifer Bridwell-Rabb,Bin Li,Catherine L Drennan
Jennifer Bridwell-Rabb
The members of the radical S-adenosylmethionine (SAM) enzyme superfamily are responsible for catalyzing a diverse set of reactions in a multitude of biosynthetic pathways. Many members of this superfamily accomplish their transformations us...
Exosome-Encased Nucleic Acid Scaffold Chemotherapeutic Agents for Superior Anti-Tumor and Anti-Angiogenesis Activity [0.03%]
一种外泌体封装核酸支架化疗药物用于增强抗肿瘤和抑制血管生成作用
Ryan P McNamara,Anthony B Eason,Yijun Zhou et al.
Ryan P McNamara et al.
Extracellular vesicles (EVs), or exosomes, play a pivotal role in tumor growth and metastasis, such as in the case of Kaposi Sarcoma. By loading tumor-derived EVs with chemotherapeutic drugs, we noted that their pro-tumor/pro-angiogenic phe...
Resolving the Multidecade-Long Mystery in MoaA Radical SAM Enzyme Reveals New Opportunities to Tackle Human Health Problems [0.03%]
揭秘莫亚A类Radical SAM酶数十年之谜发现解决人类健康问题的新机遇
Kenichi Yokoyama,Di Li,Haoran Pang
Kenichi Yokoyama
MoaA is one of the most conserved radical S-adenosyl-l-methionine (SAM) enzymes, and is found in most organisms in all three kingdoms of life. MoaA contributes to the biosynthesis of molybdenum cofactor (Moco), a redox enzyme cofactor used ...
Evolution of Methods for the Study of Cobalamin-Dependent Radical SAM Enzymes [0.03%]
钴胺素依赖的Radical SAM酶研究方法的发展变迁
Erica K Sinner,Daniel R Marous,Craig A Townsend
Erica K Sinner
While bioinformatic evidence of cobalamin-dependent radical S-adenosylmethionine (SAM) enzymes has existed since the naming of the radical SAM superfamily in 2001, none were biochemically characterized until 2011. In the past decade, the fi...
Proposed Mechanism for the Biosynthesis of the [FeFe] Hydrogenase H-Cluster: Central Roles for the Radical SAM Enzymes HydG and HydE [0.03%]
[铁铁]氢化酶H簇生物合成的提议机制——含SAM的自由基酶HydG和HydE起核心作用
R David Britt,Lizhi Tao,Guodong Rao et al.
R David Britt et al.
Radical S-adenosylmethionine (radical SAM or rSAM) enzymes use their S-adenosylmethionine cofactor bound to a unique Fe of a [4Fe-4S] cluster to generate the "hot" 5'-deoxyadenosyl radical, which drives highly selective radical reactions vi...
Binding of AP endonuclease-1 to G-quadruplex DNA depends on the N-terminal domain, Mg2+ and ionic strength [0.03%]
AP核酸内切酶-1与G-四链体DNA的结合依赖于N端结构域、Mg2+和盐浓度
Aaron M Fleming,Shereen A Howpay Manage,Cynthia J Burrows
Aaron M Fleming
The base excision repair enzyme apurinic/apyrimidinic endonuclease-1 (APE1) is also engaged in transcriptional regulation. APE1 can function in both pathways when the protein binds to a promoter G-quadruplex (G4) bearing an abasic site (mod...
A Stable Gold Nanoparticle-Based Vaccine for the Targeted Delivery of Tumor-Associated Glycopeptide Antigens [0.03%]
一种用于靶向递送肿瘤相关糖肽抗原的稳定的金纳米颗粒疫苗
Kevin R Trabbic,Kristopher A Kleski,Joseph J Barchi Jr
Kevin R Trabbic
We have developed a novel antigen delivery system based on polysaccharide-coated gold nanoparticles (AuNPs) targeted to antigen presenting cells (APCs) expressing Dectin-1. AuNPs were synthesized de-novo using yeast-derived β-1,3-glucans (...
Exploring the Effects of Incorporating Different Bioactive Phospholipids into Messenger Ribonucleic Acid Lipid Nanoparticle (mRNA LNP) Formulations [0.03%]
探讨不同生物活性磷脂对信使核糖核酸脂质纳米粒(mRNA LNP)制剂的影响
The current rapid advancement in ribonucleic acid (RNA) therapeutics research depends on innovations in drug delivery, especially the development of a lipid-nanoparticle (LNP)-based system. The conventional LNP formulation typically contain...
Metal-Triggered FAD Reduction in d-2-Hydroxyglutarate Dehydrogenase from Pseudomonas aeruginosa PAO1 [0.03%]
绿假单胞菌PAO1来源的d-2-羟基戊二酸脱氢酶中的金属离子触发的FAD还原反应
Alcohol oxidation is an indispensable chemical reaction in biological systems. This process, biologically catalyzed by alcohol dehydrogenases (ADHs) and alcohol oxidases (AOXs), follows two distinct chemical routes depending on the cofactor...