Proposed Mechanism for the Biosynthesis of the [FeFe] Hydrogenase H-Cluster: Central Roles for the Radical SAM Enzymes HydG and HydE [0.03%]
[铁铁]氢化酶H簇生物合成的提议机制——含SAM的自由基酶HydG和HydE起核心作用
R David Britt,Lizhi Tao,Guodong Rao et al.
R David Britt et al.
Radical S-adenosylmethionine (radical SAM or rSAM) enzymes use their S-adenosylmethionine cofactor bound to a unique Fe of a [4Fe-4S] cluster to generate the "hot" 5'-deoxyadenosyl radical, which drives highly selective radical reactions vi...
Binding of AP endonuclease-1 to G-quadruplex DNA depends on the N-terminal domain, Mg2+ and ionic strength [0.03%]
AP核酸内切酶-1与G-四链体DNA的结合依赖于N端结构域、Mg2+和盐浓度
Aaron M Fleming,Shereen A Howpay Manage,Cynthia J Burrows
Aaron M Fleming
The base excision repair enzyme apurinic/apyrimidinic endonuclease-1 (APE1) is also engaged in transcriptional regulation. APE1 can function in both pathways when the protein binds to a promoter G-quadruplex (G4) bearing an abasic site (mod...
A Stable Gold Nanoparticle-Based Vaccine for the Targeted Delivery of Tumor-Associated Glycopeptide Antigens [0.03%]
一种用于靶向递送肿瘤相关糖肽抗原的稳定的金纳米颗粒疫苗
Kevin R Trabbic,Kristopher A Kleski,Joseph J Barchi Jr
Kevin R Trabbic
We have developed a novel antigen delivery system based on polysaccharide-coated gold nanoparticles (AuNPs) targeted to antigen presenting cells (APCs) expressing Dectin-1. AuNPs were synthesized de-novo using yeast-derived β-1,3-glucans (...
Exploring the Effects of Incorporating Different Bioactive Phospholipids into Messenger Ribonucleic Acid Lipid Nanoparticle (mRNA LNP) Formulations [0.03%]
探讨不同生物活性磷脂对信使核糖核酸脂质纳米粒(mRNA LNP)制剂的影响
The current rapid advancement in ribonucleic acid (RNA) therapeutics research depends on innovations in drug delivery, especially the development of a lipid-nanoparticle (LNP)-based system. The conventional LNP formulation typically contain...
Metal-Triggered FAD Reduction in d-2-Hydroxyglutarate Dehydrogenase from Pseudomonas aeruginosa PAO1 [0.03%]
绿假单胞菌PAO1来源的d-2-羟基戊二酸脱氢酶中的金属离子触发的FAD还原反应
Alcohol oxidation is an indispensable chemical reaction in biological systems. This process, biologically catalyzed by alcohol dehydrogenases (ADHs) and alcohol oxidases (AOXs), follows two distinct chemical routes depending on the cofactor...