Fragment-Based Ab Initio Phasing of Peptidic Nanocrystals by MicroED [0.03%]
基于片段的微ED ab initio相位法测定肽纳米晶体结构
Logan S Richards,Maria D Flores,Claudia Millán et al.
Logan S Richards et al.
Electron diffraction (MicroED/3DED) can render the three-dimensional atomic structures of molecules from previously unamenable samples. The approach has been particularly transformative for peptidic structures, where MicroED has revealed no...
Sex-Specific Silica Nanoparticle Protein Corona Compositions Exposed to Male and Female BALB/c Mice Plasmas [0.03%]
性别特异性二氧化硅纳米颗粒蛋白壳成分的组成Exposed to Male and Female BALB/c小鼠血浆暴露
Ali Akbar Ashkarran,Hassan Gharibi,Jason W Grunberger et al.
Ali Akbar Ashkarran et al.
As various nanoparticles (NPs) are increasingly being used in nanomedicine products for more effective and less toxic therapy and diagnosis of diseases, there is a growing need to understand their biological fate in different sexes. Herein,...
Platelet Response to Allergens, CXCL10, and CXCL5 in the Context of Asthma [0.03%]
哮喘背景下过敏原、CXCL10和CXCL5诱导的血小板反应
Sarah Gruba,Xiaojie Wu,Eleni Spanolios et al.
Sarah Gruba et al.
Asthma is a chronic respiratory disease initiated by a variety of factors, including allergens. During an asthma attack, the secretion of C-X-C-motif chemokine 10 (CXCL10) and chemokine ligand 5 (CCL5) causes the migration of immune cells, ...
Improved Yield for the Enzymatic Synthesis of Radiolabeled Nicotinamide Adenine Dinucleotide [0.03%]
改善酶促合成放射性标记烟酰胺腺嘌呤二核苷酸的产率
Jared Eller,Shivansh Goyal,Xiaolu A Cambronne
Jared Eller
Labeled β-nicotinamide adenine dinucleotide (NAD) analogues have been critical for uncovering new biochemical connections and quantitating enzymatic activity. They function as tracers for enzymology, flux analyses, and in situ measurements...
Activity-Based Sensing for Chemistry-Enabled Biology: Illuminating Principles, Probes, and Prospects for Boronate Reagents for Studying Hydrogen Peroxide [0.03%]
基于活性的传感在化学驱动生物学中的应用：阐明用于研究过氧化氢的硼试剂的原则、探针及前景
Marco S Messina,Gianluca Quargnali,Christopher J Chang
Marco S Messina
Activity-based sensing (ABS) offers a general approach that exploits chemical reactivity as a method for selective detection and manipulation of biological analytes. Here, we illustrate the value of this chemical platform to enable new biol...
Adhesive Virulence Factors of Staphylococcus aureus Resist Digestion by Coagulation Proteases Thrombin and Plasmin [0.03%]
粘附的金黄色葡萄球菌毒力因素抵抗凝血蛋白酶-凝血酶和纤溶酶的分解消化
Fanny Risser,Joanan López-Morales,Michael A Nash
Fanny Risser
Staphylococcus aureus (S. aureus) is an invasive and life-threatening pathogen that has undergone extensive coevolution with its mammalian hosts. Its molecular adaptations include elaborate mechanisms for immune escape and hijacking of the ...
Unraveling the Chemistry of meso-Cl Tricarbocyanine Dyes in Conjugation Reactions for the Creation of Peptide Bonds [0.03%]
解开meso-Cl三碳氰染料在连接反应中创建肽键的化学原理
Rüdiger M Exner,Fernando Cortezon-Tamarit,Haobo Ge et al.
Rüdiger M Exner et al.
Tricarbocyanine dyes have become popular tools in life sciences and medicine. Their near-infrared (NIR) fluorescence makes them ideal agents for imaging of thick specimens or in vivo imaging, e.g., in fluorescence-guided surgery. Among othe...
Dual-Reporter System for Real-Time Monitoring of SARS-CoV-2 Main Protease Activity in Live Cells Enables Identification of an Allosteric Inhibition Path [0.03%]
用于实时监测活细胞中SARS-CoV-2主蛋白酶活性的双报告系统实现了对变构抑制途径的识别
Yaron Bram,Xiaohua Duan,Benjamin E Nilsson-Payant et al.
Yaron Bram et al.
The SARS-CoV-2 pandemic is an ongoing threat to global health, and the continuing emergence of contagious variants highlights the urgent need for additional antiviral therapy to attenuate COVID-19 disease. The SARS-CoV-2 main protease (3CLp...
Substrate Specificity and Kinetics of RNA Hydrolysis by SARS-CoV-2 NSP10/14 Exonuclease [0.03%]
SARS-CoV-2 NSP10/14 exonuclease对RNA水解的底物特异性和动力学研究
Tyler L Dangerfield,Kenneth A Johnson
Tyler L Dangerfield
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the virus that causes COVID-19, continues to evolve resistance to vaccines and existing antiviral therapies at an alarming rate, increasing the need for new direct-acting antivir...
In Vitro Demonstration of Human Lipoyl Synthase Catalytic Activity in the Presence of NFU1 [0.03%]
NFU1存在下的人类脂氧合成酶催化活性的体外演示
Douglas M Warui,Debangsu Sil,Kyung-Hoon Lee et al.
Douglas M Warui et al.
Lipoyl synthase (LS) catalyzes the last step in the biosynthesis of the lipoyl cofactor, which is the attachment of sulfur atoms at C6 and C8 of an n-octanoyllysyl side chain of a lipoyl carrier protein (LCP). The protein is a member of the...