Ivan Corbeski,Rajiv Kumar Bedi,Christian M Matter et al.
Ivan Corbeski et al.
The complex of methyltransferase-like proteins 3 and 14 (METTL3-14) is the main human enzyme that deposits the most abundant internal mRNA modification, N6-methyladenosine (m6A). In the heterodimeric complex, METTL3 acts as a catalytic subu...
CryoEM Structures of Native Quinol-Dependent Nitric Oxide Reductase in Resting and Quinol-Bound States [0.03%]
休息态和辅酶Q结合态的原生醌依赖型一氧化氮还原酶的低温电子显微镜结构
Faisal T Khaja,Allegra Mboukou,Louie P Aspinall et al.
Faisal T Khaja et al.
The membrane-bound quinol-dependent nitric oxide reductases (qNORs), which are members of the respiratory heme-copper oxidase superfamily, are of major importance to food production, environment, and human health. They are unique to bacteri...
High Drug Encapsulation Capacity in Cyclodextrin-Based Nanoparticles: Characterization and In Vivo Antitumor Efficacy in Tumor-Bearing Mice [0.03%]
肿瘤小鼠体内 cyclodextrin 纳米粒的高载药能力、性质及抗肿瘤活性研究
Tomoki Kosugi,Mina Sakuragi,Madoka Toya et al.
Tomoki Kosugi et al.
Insufficient drug encapsulation, typically at about 10 wt %, remains a major challenge in the design of nanotechnology-based drug delivery systems. In this study, we created cyclodextrin-based nanoparticles (CDNP_PEGs), synthesized by the h...
MAPK Pathway Inhibition Reshapes Kinase Chemical Probe Reactivity Reflecting Cellular Activation States [0.03%]
MAPK通路抑制重塑激酶化学探针活性反映细胞活化状态
Andrew F Jarvis,Mohd Younis Bhat,Timothé Maujean et al.
Andrew F Jarvis et al.
Despite the pivotal role of oncogenic kinases in cancer initiation, progression, and therapeutic resistance, functionally profiling their activity and conformational dynamics in live cells remains challenging. Existing methods often fail to...
Structural and Biochemical Characterization of Fusobacterium nucleatum Enoyl-ACP Reductase II (FabK) Reveals the Basis for Bacterial Species-Specific Inhibition [0.03%]
核糖体和生化特征鉴定福斯杆菌核杆菌酰基载体蛋白烯醇酶II（Fabk）揭示细菌物种特异性抑制基础
Kristiana Avad,Osama Alaidi,Destiny Okpomo et al.
Kristiana Avad et al.
Fusobacterium nucleatum is a Gram-negative anaerobic bacterium ubiquitous in the oral cavity and increasingly recognized for its involvement in diverse clinical conditions, including periodontal disease, inflammatory bowel disease, prematur...
Othman Al Musaimi,Daryl R Williams
Othman Al Musaimi
A significant advancement in Merrifield's classic solid-phase peptide synthesis (SPPS) that greatly expands the scope of accessible peptide structures is reported here. Building upon the one-bead, one-compound (OBOC) concept, this approach ...
Photoinduced Electron Transfer Informs on Pathway Coupling in Flavin-Based Electron Bifurcation [0.03%]
光诱导电子转移揭示黄素介导的电子分流中途径耦合机制
Seth A Wiley,Carolyn E Lubner
Seth A Wiley
Flavin-based electron bifurcation (FBEB) is an enzymatic mechanism that generates extremely high-energy electrons to drive unfavorable chemical reactions. It is utilized by the NADH-dependent ferredoxin:NADP+-oxidoreductase (Nfn) enzyme in ...
Optimization of Indole- and Pyrazole-fused Glycyrrhetinic Acid Derivatives as Potent PTP1B Inhibitors: In Silico, In Vitro, In Vivo, and Metabolomic Studies [0.03%]
优化异吲哚和吡唑融合甘草酸衍生物作为高效PTP1B抑制剂：基于计算机、体外、体内及 metaboomics研究
Mitzi López-Sánchez,Hannya Mendoza-Mota,Ledy De-la-Cruz-Martínez et al.
Mitzi López-Sánchez et al.
Protein tyrosine phosphatase 1B (PTP1B) is a crucial enzyme involved in regulating insulin and leptin signaling pathways, making it a promising target for treating type 2 diabetes. In this study, we synthesized 14 derivatives of indole- and...
Jacob Kress,Sookyeong Kim,Caitlynn Bryant et al.
Jacob Kress et al.
The development of genetically encoded labels for multicolor experiments requires a diverse palette of fluorescent proteins that are well-behaved. Here, we report mSumireF, a monomeric variant of the violet fluorescent protein Sumire. On it...
Is Thiourea the Weak Link? An Investigation of the In Vitro and In Vivo Destabilization of [203Pb]- and [212Pb]Pb2+ Thiourea-Based Bioconjugates [0.03%]
硫脲是薄弱环节吗？关于[203Pb]-和[212Pb]Pb2+ 硫脲基生物结合物的体外及体内去稳定作用的调查研究
Brooke L McNeil,Luke Wharton,Chao-Cheng Chen et al.
Brooke L McNeil et al.
Fibroblast activation protein (FAP) is overexpressed in a variety of cancers, making it an attractive target for bifunctional chelator-based radiopharmaceuticals. This study initially aimed to assess the effect of chelator structure on the ...