Bacterial Cytochrome P450 Catalyzed Macrocyclization of Ribosomal Peptides [0.03%]
核糖体肽的细菌细胞色素P450介导的宏环化反应
Jing Liu,Runze Liu,Bei-Bei He et al.
Jing Liu et al.
Macrocyclization is a vital process in the biosynthesis of ribosomally synthesized and post-translationally modified peptides (RiPPs), significantly enhancing their structural diversity and biological activity. Universally found in living o...
Chlorophyllase from Arabidopsis thaliana Reveals an Emerging Model for Controlling Chlorophyll Hydrolysis [0.03%]
拟南芥叶绿素酶揭示了控制叶绿素水解的新模型
Madison Knapp,Minshik Jo,Courtney L Henthorn et al.
Madison Knapp et al.
Chlorophyll (Chl) is one of Nature's most complex pigments to biosynthesize and derivatize. This pigment is vital for survival and also paradoxically toxic if overproduced or released from a protective protein scaffold. Therefore, along wit...
Bioinformatics-Facilitated Identification of Novel Bacterial Sulfoglycosidases That Hydrolyze 6-Sulfo- N-acetylglucosamine [0.03%]
基于生物信息学的新型细菌硫化糖苷酶发现及鉴定：从基因到功能
Mochen Dong,Zhuoyun Chen,Yuan He et al.
Mochen Dong et al.
Glycan sulfation is a widespread postglycosylation modification crucial for modulating biological functions including cellular adhesion, signaling, and bacterial colonization. 6-Sulfo-β-GlcNAcases are a class of enzyme that alters sulfatio...
Design, Synthesis, and Biological Evaluation of Darunavir Analogs as HIV-1 Protease Inhibitors [0.03%]
作为HIV-1蛋白酶抑制剂的达芦那韦类似物的设计、合成及生物活性评价
Muhammad Asad Ur Rehman,Hathaichanok Chuntakaruk,Soraat Amphan et al.
Muhammad Asad Ur Rehman et al.
Darunavir, a frontline treatment for HIV infection, faces limitations due to emerging multidrug resistant (MDR) HIV strains, necessitating the development of analogs with improved activity. In this study, a combinatorial in silico approach ...
Development of a Polymersome Blood Ammonia Assay Coupled with a Portable Near-Infrared Fluorometer [0.03%]
便携式近红外荧光仪聚合物血氨测定法的发展
Marie-Lynn Al-Hawat,Justine Caron,Sarah Djebbar et al.
Marie-Lynn Al-Hawat et al.
Ammonia is a key biomarker in inborn and acquired liver disease. As clinical point-of-care blood ammonia assays are lacking, we developed a polymersome formulation for point-of-care blood ammonia sensing combined with a portable fluorometer...
New Catalytic Residues and Catalytic Mechanism of the RNase T1 Family [0.03%]
RNase T1家族的新催化残基及其催化机制
Katsuki Takebe,Mamoru Suzuki,Yumiko Hara et al.
Katsuki Takebe et al.
The ribonuclease T1 family, including RNase Po1 secreted by Pleurotus ostreatus, exhibits antitumor activity. Here, we resolved the Po1/guanosine-3'-monophosphate complex (3'GMP) structure at 1.75 Å. Structure comparison and fragment molec...
Directed Evolution of Candidatus Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase for the Genetic Incorporation of Two Different Noncanonical Amino Acids in One Protein [0.03%]
卡氏甲基营养产甲烷菌乙酸裂解酶蛋白合成中两种非标准氨基酸的遗传密码重编程及定向进化研究
Chia-Chuan D Cho,Waye Michelle Leeuwon,Wenshe Ray Liu
Chia-Chuan D Cho
The genetic code expansion technique is a powerful chemical biology tool to install noncanonical amino acids (ncAAs) in proteins. As a key enzyme for this technique, pyrrolysyl-tRNA synthetase (PylRS), coupled with its cognate amber suppres...
Bioproduction Platform to Generate Functionalized Disulfide-Constrained Peptide Analogues [0.03%]
用于生成功能化二硫键约束肽类似物的生物生产平台
Sunhee Hwang,Aaron T Balana,Bryan Martin et al.
Sunhee Hwang et al.
Disulfide-constrained peptides (DCPs) have gained increased attention as a drug modality due to their exceptional stability and combined advantages of large biologics and small molecules. Chemical synthesis, although widely used to produce ...
Selagibenzophenone B and Its Derivatives: SelB-1, a Dual Topoisomerase I/II Inhibitor Identified through In Vitro and In Silico Analyses [0.03%]
从体内外研究中发现的番杏苯酮B及其衍生物：SelB-1是一种双重拓扑异构酶I/II抑制剂
Serhat Dönmez,Ringaile Lapinskaite,Hazal Nazlican Atalay et al.
Serhat Dönmez et al.
The development of multitargeted drugs represents an innovative approach to cancer treatment, aiming to enhance drug effectiveness while minimizing side effects. Herein, we sought to elucidate the inhibitory effect of selagibenzophenone B d...
Glycopolymers Prepared by Alternating Ring-Opening Metathesis Polymerization Provide Access to Distinct, Multivalent Structures for the Probing of Biological Activity [0.03%]
通过交替的开环元聚合制备的糖类聚合物提供了用于探测生物活性的不同多价结构的途径
Luz C Mendez,Francis O Boadi,Mitchell Kennedy et al.
Luz C Mendez et al.
A myriad of biological processes are facilitated by ligand-receptor interactions. The low affinities of these interactions are typically enhanced by multivalent engagements to promote binding. However, each biological interaction requires a...