Exploring the Trans-Cleavage Activity with Rolling Circle Amplification for Fast Detection of miRNA [0.03%]
基于滚动环式扩增技术的miRNA转切割检测方法研究
Chenqi Niu,Juewen Liu,Xinhui Xing et al.
Chenqi Niu et al.
MicroRNAs (miRNAs) are a class of endogenous short noncoding RNA. They regulate gene expression and function, essential to biological processes. It is necessary to develop an efficient detection method to determine these valuable biomarkers...
Phages and Nanotechnology: New Insights against Multidrug-Resistant Bacteria [0.03%]
噬菌体和纳米技术：针对多重耐药细菌的新见解
Marco Pardo-Freire,Pilar Domingo-Calap
Marco Pardo-Freire
Bacterial infections are a major threat to the human healthcare system worldwide, as antibiotics are becoming less effective due to the emergence of multidrug-resistant strains. Therefore, there is a need to explore nontraditional antimicro...
Ling Liu,Shimaa Elsayed Helal,Nan Peng
Ling Liu
Probiotics are the treasure of the microbiology fields. They have been widely used in the food industry, clinical treatment, and other fields. The equivocal health-promoting effects and the unknown action mechanism were the largest obstacle...
qSanger: Quantification of Genetic Variants in Bacterial Cultures by Sanger Sequencing [0.03%]
基于桑格测序的细菌培养物中遗传变异定量的方法(qSanger)
Satya Prakash,Adrian Racovita,Teresa Petrucci et al.
Satya Prakash et al.
Genetic variations such as mutations and recombinations arise spontaneously in all cultured organisms. Although it is possible to identify nonneutral mutations by selection or counterselection, the identification of neutral mutations in a h...
Emily G Brooks,Estefania Elorriaga,Yang Liu et al.
Emily G Brooks et al.
High-precision bioengineering and synthetic biology require fine-tuning gene expression at both transcriptional and posttranscriptional levels. Gene transcription is tightly regulated by promoters and terminators. Promoters determine the ti...
Atligator Web: A Graphical User Interface for Analysis and Design of Protein-Peptide Interactions [0.03%]
Atligator Web：蛋白质-肽相互作用分析和设计的图形用户界面
Josef Paul Kynast,Birte Höcker
Josef Paul Kynast
A key functionality of proteins is based on their ability to form interactions with other proteins or peptides. These interactions are neither easily described nor fully understood, which is why the design of specific protein-protein intera...
Peptide Variant Detection by a Living Yeast Biosensor via an Epitope-Selective Protease [0.03%]
通过表位选择性蛋白酶利用活酵母生物传感器检测肽变异体
Tea Crnković,Benjamin J Bokor,Mead E Lockwood et al.
Tea Crnković et al.
We previously demonstrated that we could hijack the fungal pheromone signaling pathway to provide a living yeast biosensor where peptide biomarkers were recognized by G-protein-coupled receptors and engineered to transcribe a readout. Here,...
De Novo Design and Synthesis of Polypeptide Immunomodulators for Resetting Macrophage Polarization [0.03%]
从头设计和合成用于重置巨噬细胞极化的多肽免疫调节剂
Na Kong,Hongru Ma,Zhongji Pu et al.
Na Kong et al.
Modulating the extracellular matrix microenvironment is critical for achieving the desired macrophage phenotype in immune investigations or tumor therapy. Combining de novo protein design and biosynthesis techniques, herein, we designed a b...
Revealing the Host-Dependent Nature of an Engineered Genetic Inverter in Concordance with Physiology [0.03%]
生理学相一致的宿主依赖型工程遗传逆变器的研究
Dennis Tin Chat Chan,Geoff S Baldwin,Hans C Bernstein
Dennis Tin Chat Chan
Broad-host-range synthetic biology is an emerging frontier that aims to expand our current engineerable domain of microbial hosts for biodesign applications. As more novel species are brought to "model status," synthetic biologists are disc...
Ying-Chiang J Lee,Xuanqi Chen,Siddharth Marwaha
Ying-Chiang J Lee
The growth of biotechnology in recent decades and the dual-use nature of most bioscience research are making their misuse, or accidental misuse or release, more likely and present as threats to biosecurity. A proactive approach is through e...