Microbial-type terpene synthases enable enhanced insect and fungal resistance in engineered plants [0.03%]
微生物萜类合酶使工程植物具备更佳的抗虫和抗真菌性能
Saif Ul Malook,Xinlu Chen,Bode A Olukolu et al.
Saif Ul Malook et al.
A major challenge in crop improvement is enhancing resistance to diverse biotic stresses. Because terpenoids play key roles in chemical defense, an envisioned strategy is to introduce new terpene metabolic pathways into crops through engine...
DeepCodon: A deep learning codon-optimization model to enhance protein expression [0.03%]
深度密码子：一种增强蛋白质表达的深度学习密码子优化模型
Xudong Han,Xiaotong Shao,Shuo Liu et al.
Xudong Han et al.
Codon optimization enhances heterologous gene expression by modulating synonymous codon usage, a critical task in genetic engineering and synthetic biology. Achieving optimal expression requires balancing multiple interdependent factors, su...
Engineering Escherichia coli for the S-selective production of 2-hydroxyisovalerate [0.03%]
工程改造大肠杆菌实现S-选择性生产2-羟基异戊酸
Peiling Wu,Haofeng Chen,Guojun Yang et al.
Peiling Wu et al.
2-Hydroxyisovalerate (2-HIV) is a value-added chemical that is widely applied in the synthesis of bioactive compounds and polymers. Here, we report an underexplored metabolic route for the de novo production of S-type 2-HIV (S-HIV) in Esche...
Protein design drives synthetic biology research of plant natural products [0.03%]
蛋白质设计推动植物天然产物合成生物学研究
Xiaopeng Zhang,Yinying Yao,Ye Wang et al.
Xiaopeng Zhang et al.
Plant natural products (PNPs) are widely used in pharmaceutical, cosmetic, and food industries. Currently, their commercial supply largely relies on plant extraction, yet, suffers from low yield and limited resources. Synthetic biology prov...
Advances in the microbial biosynthesis of ʟ-tryptophan and its derivatives [0.03%]
微生物合成左旋色氨酸及其衍生物研究进展
Honghao Li,Jiaming Zhang,Jiaxin Gao et al.
Honghao Li et al.
l-Tryptophan, an essential aromatic amino acid, is widely used in the pharmaceutical and food industries owing to its distinctive indole ring structure, which is unique among proteinogenic amino acids. Moreover, structurally modified deriva...
Four enzymes from button bush enable de novo biosynthesis of spirooxindole alkaloids in yeast [0.03%]
四种来自木蝴蝶的酶使.spirooxindole类生物碱从头合成途径在酵母中得以实现
Alyssa Dawn Seveck,Jacob Owen Perley,Di Gao et al.
Alyssa Dawn Seveck et al.
Oxindoles are a structurally unique subclass of monoterpenoid indole alkaloids (MIAs) with significant medicinal values such as neuroprotective and anticancer properties and are predominantly found in the Rubiaceae plant family. Recent rese...
De novo biosynthesis of Astragalus bioactive isoflavonoid calycosin-7-glucoside in yeast [0.03%]
从酵母中去 novo 生物合成 astragalus 活性异黄酮(calycosin-7-glucoside)
Cong Chen,Tengfei Liu,Ying Huang et al.
Cong Chen et al.
Isoflavonoids, such as calycosin-7-glucoside, possess significant pharmaceutical value but are typically sourced from plants, where production is limited by slow growth and complex isolation process. Here, we engineered Saccharomyces cerevi...
Metabolic engineering of Escherichia coli for de novo production of lauryl glucoside [0.03%]
大肠杆菌从头产生月桂基葡糖苷的代谢工程
Kasimaporn Promubon,Chaiwat Arjin,Chayakorn Pumas et al.
Kasimaporn Promubon et al.
Lauryl glucoside, a non-ionic surfactant used in various cosmetic products, is valued for its biodegradability and gentleness on the skin. However, its current production through chemical synthesis is considered unsustainable, necessitating...
Identification and engineering of a UDP-rhamnosyltransferase from Trillium tschonoskii for heterologous biosynthesis of polyphyllin Ⅱ in yeast [0.03%]
东京小苗UDP-半乳糖醛酸吡喃糖基转移酶的鉴定与改造及其在酵母中异源合成大黄素甲醚聚苯乙烯胺Ⅱ
Yuxin Yang,Haowen Wang,Ziya Wu et al.
Yuxin Yang et al.
Polyphyllins, a class of isospirostan-type steroidal saponins, exhibit cytotoxicity against a wide range of cancer cells. Despite extensive research, the complete biosynthetic pathway of these compounds remains elusive. To investigate these...
Advances in luminescence-based tracing technologies: applications and future perspectives [0.03%]
发光示踪技术研究进展：应用与展望
Siyuan Sun,Tiange Wang,Yingzi Li et al.
Siyuan Sun et al.
Luminescence-based imaging is a fundamental tool for visualizing dynamic biological processes, primarily through bioluminescence, which generates light via enzyme-substrate reactions, and fluorescence, which requires external excitation. Th...