Cellulose saccharification to alleviate CCR of xylose for enhanced 1, 2, 4-butanetriol production [0.03%]
纤维素糖化缓解木糖CCR以增强1,2,4-丁三醇的生产
Shengji Li,Lisha Qin,Mengyue Ge et al.
Shengji Li et al.
1,2,4-Butanetriol (BT) is a valuable polyol with applications in pharmaceuticals, materials, and military sectors. BT biosynthesis primarily uses xylose as substrate, with glucose supporting cell growth. However, glucose-mediated carbon cat...
SynBioGPT2: A dynamic reasoning framework enables high-fidelity design of microbial cell factories [0.03%]
SynBioGPT2：一种动态推理框架，可实现微生物细胞工厂的高保真设计
Zhitao Mao,Jun Du,Jirun Guan et al.
Zhitao Mao et al.
The rational design of microbial cell factories is essential for sustainable biomanufacturing, yet the traditional Design-Build-Test-Learn (DBTL) cycle is bottlenecked by the highly non-linear and interconnected nature of biological systems...
Toward development of soil-derived Bacillus isolates as lung cancer cytotoxic agents [0.03%]
土壤芽孢杆菌菌株作为抗癌药物的开发研究进展
Dhruba Deb,Filippo Liguori,Bentley M Shuster et al.
Dhruba Deb et al.
The wide-ranging impact of the human microbiome on health and disease has sparked growing interest in employing bacteria as live therapeutics. Natural properties of bacteria have been enhanced using synthetic biology to treat diverse diseas...
Enhanced growth of 1-butanol producing Escherichia coli via a metabolic buffer [0.03%]
通过代谢缓冲实现产1-丁醇工程大肠杆菌的高效生长
Haidong Yu,Haofeng Chen,Guojun Yang et al.
Haidong Yu et al.
1-Butanol is a C4 aliphatic alcohol that is widely used as a gasoline blendstock. In this study, we report an underexplored metabolic route for the de novo biosynthesis of 1-butanol in Escherichia coli. Specifically, the 1-butanol biosynthe...
Prime editing: Emerging mechanisms, engineering innovations, and next-generation applications [0.03%]
prime编辑：新兴机制，工程创新及下一代应用
Waqar Muhammad,Ali Amjad,Yufei Liu et al.
Waqar Muhammad et al.
Prime editing has become a highly programmable and accurate genome-editing platform that can install targeted substitutions, insertions, and deletions without introducing double-strand breaks or requiring a separate donor DNA template. This...
RecN: A tunable switch for DNA repair choice and stress tolerance in Zymomonas mobilis [0.03%]
RecN：Zymomonas mobilis 中可调节的DNA修复切换蛋白以响应不同的胁迫压力
Saifu Pan,Xiaojie Wang,Jingjing He et al.
Saifu Pan et al.
Engineering polyploid industrial microorganisms is hindered by their intrinsic capacity to repair induced mutations, limiting the efficiency of genome editing and directed evolution. Using the ethanologenic bacterium Zymomonas mobilis- a po...
Archaea-driven bioremediation of polyolefins and polyesters in extreme environments [0.03%]
极端环境下的古菌驱动聚烯烃和聚酯的生物修复作用
Aubrey Dickson Chigwada,Memory Tekere
Aubrey Dickson Chigwada
Global plastic production surpassed 436 million metric tonnes in 2023, with polyolefins, polyethylene and polypropylene, and polyesters, polyethylene terephthalate and polybutylene adipate terephthalate dominating the persistent fraction. I...
Laticifer-specific engineering of rubber biosynthesis in Taraxacum kok-saghyz [0.03%]
唐古特乌头胶乳生物合成的橡胶弹性蛋白特异性工程化改造
Xue Yang,Junfeng Cao,Zhonghua Li et al.
Xue Yang et al.
Rubber dandelion (Taraxacum kok-saghyz, TKS) is a promising alternative source of natural rubber, which is synthesized in specialized laticifer cells. While genetic engineering of TKS holds promise for enhancing rubber yield, constitutive t...
High-resolution melting analysis for rapid and cost-effective detection of unnatural base pairs [0.03%]
高分辨率溶解曲线分析技术——快速经济地检测非天然碱基对
Sophie E J van der Vlugt,Jelke J Fros,Vitor A P Martins Dos Santos et al.
Sophie E J van der Vlugt et al.
Expansion of the genetic alphabet with unnatural base pairs (UBPs) enables new approaches in protein engineering, synthetic biology, and biocontainment. Such applications rely on faithful incorporation and retention of UBPs in semi-syntheti...
Xi Sun,Xinyun Yi,Jia Zhang
Xi Sun
Sauce-flavor Baijiu owes its layered aroma to a diverse microbial consortium fermenting under extreme, open, solid-state conditions. High-throughput sequencing has revealed marked spatiotemporal heterogeneity in community composition, yet a...