An Open-Source Workflow for Semi-Automated Spatial Profiling of Multiplex Immunofluorescent Images [0.03%]
一种开源工作流程：对多重免疫荧光图像进行半自动空间分析
Alisha Dabb,Cynthia Morgan,Sophia Noble et al.
Alisha Dabb et al.
Multiplex immunofluorescence microscopy facilitates the quantification and spatial analysis of cellular features within tissue sections, allowing greater understanding of disease progression or the effects of drug treatment or other exposur...
How to Apply Social Network Analysis to Evaluate Professional Development Programs in Biomedical Research [0.03%]
如何运用社会网络分析评估生物医学研究中的职业发展项目
Diane B Smith,Carolyn J Hovde,Julia Thom Oxford
Diane B Smith
Effective evaluation and assessment of research-focused programs, including centers, core facilities, and institutes, is crucial but inherently challenging. Faculty members are often departmentalized, yet their research necessitates campus-...
Antisense Oligonucleotide Pulldown and Silencing of Circular RNA Nfix In Vivo in Neonatal Mouse Lungs [0.03%]
circNfix的反义寡核苷酸沉淀及其在新生小鼠肺部的体内干扰作用
Pragnya Das,Sharmistha Shyamal,Vineet Bhandari et al.
Pragnya Das et al.
RNA-based therapeutics are emerging as a powerful platform for disease treatment, and one of the novel RNA molecules with therapeutic potential is circular RNA (circRNA). The ubiquitously expressed circRNAs are covalently closed, single-str...
A Semi-Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels [0.03%]
一种半定量酵母互补平台 用于表征膜通道的尿素和氨转运能力
Anna Stoib,Sahar Shojaei,Christine Siligan et al.
Anna Stoib et al.
Yeast complementation assays provide a robust in vivo platform for characterizing the permeability and pH gating of transmembrane channels. This article details a liquid culture approach to quantify urea and ammonia transport using Saccharo...
An Optimized Protocol for the Generation of iPSC Lines from Lymphoblastoid Cell Lines Using the Non-integrative Sendai Virus [0.03%]
一种使用非整合性赛代氏病毒从淋巴母细胞系中生成iPSC系的优化方案
Laurie Martineau,Isabella Bienjonetti,Sabrina Bellenfant et al.
Laurie Martineau et al.
The choice of cell source for generating induced pluripotent stem cells (iPSCs) is influenced by several factors, including accessibility, reprogramming efficiency, and the invasiveness of the cell collection procedure. Traditional sources ...
Metagenomic Hi‑C Protocols for Viral Genome Binning, Taxonomic Annotation, and Interaction Network Visualization [0.03%]
元基因组Hi-C文库构建、病毒分类和互作网络解析技术及应用
Yuxuan Du,Yuqiu Wang,Fengzhu Sun
Yuxuan Du
Metagenomic Hi-C (metaHi-C) links mobile genetic elements to their cellular hosts directly within complex microbial communities. Once shotgun and Hi-C libraries have been generated, however, the main challenges shift to the bioinformatics r...
Assessment of Bone Regeneration in Rabbit Calvarial Defects Treated With Mineralized Collagen-Based Scaffolds Using In Vivo MicroCT and Three-Dimensional Rendering [0.03%]
基于矿化胶原的骨支架兔颅顶缺损修复效果的体内micro-CT与三维重建评估研究
Youngnam Kang,Kaavian Shariati,Catherine T Cascavita et al.
Youngnam Kang et al.
Cranial bone regeneration requires coordinated interactions among host cells, extracellular matrices, and the local biomechanical environment to restore both mineralized tissue and structural protection of the brain. Mineralized collagen-gl...
Editorial: How to Apply Social Network Analysis to Evaluate Professional Development Programs in Biomedical Research [0.03%]
社论：如何运用社会网络分析评估生物医学研究的职业发展项目
Julia Thom Oxford
Julia Thom Oxford
This editorial accompanies the protocol paper entitled How to apply social network analysis to evaluate professional development programs in biomedical research. © 2026 Wiley Periodicals LLC. ...
Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi-Marker Amplicon Panel [0.03%]
适用于现场部署的多重标记扩增子文库对裂体吸虫种类和杂种进行基因分型
Oluwaremilekun Grace Ajakaye,Michael E Grigg
Oluwaremilekun Grace Ajakaye
Schistosomes are parasitic trematode worms of the genus Schistosoma, responsible for causing urogenital and intestinal schistosomiasis. Six primary species infect humans: S. mansoni, S. japonicum, S. mekongi, S. intercalatum, S. guineensis,...
Yao Lu,Khoi Nguyen Nguyen,Jianguo Xia
Yao Lu
MicrobiomeNet (https://microbiomenet.com) is a web-based platform developed to provide functional insights into microbiome signatures using genome-scale metabolic models (GEMs). It currently hosts 12,400 GEMs and around 6 million microbial ...