Reduction and Refinement of Genetically Altered Mouse Breeding: Controlling Quality and Quantity According to Experimental Needs [0.03%]
转基因小鼠繁殖的简约化和精细化——实验需求为导向的质量与数量控制
Karim Mesbah,Abdelkader Ayadi
Karim Mesbah
The mouse is currently the most widely used animal model for scientific research worldwide. In Europe, statistical data show that half of all mice used in research are genetically modified, and that 60% of the transgenic mice produced are u...
Bilcag Akgun,Farid Rajabli
Bilcag Akgun
Genetic ancestry inference has become essential in population and medical genetics, especially for studies of admixed populations. Accurate determination of both global ancestry (GA) proportions and local ancestry (LA) segmental origins req...
Streamline Protocol for Bulk-RNA Sequencing: From Data Extraction to Expression Analysis [0.03%]
简化版的RNA测序协议：从数据提取到表达分析
Abdullah Al Mohit,Niher Ranjan Das,Arushi Jain et al.
Abdullah Al Mohit et al.
Next-generation RNA sequencing (RNA-seq) allows researchers to study gene expression across the whole genome. However, its analysis often needs powerful computers and advanced command-line skills, which can be challenging when resources are...
Incorporating Scale Uncertainty into Differential Expression Analyses Using ALDEx2 [0.03%]
利用ALDEx2将尺度不确定性纳入差异表达分析中
Scott J Dos Santos,Gregory B Gloor
Scott J Dos Santos
Differential abundance or expression analyses are routinely performed on metagenomic, metatranscriptomic, and amplicon sequencing data. In such datasets, analysts usually have no information regarding the true scale (i.e., size) of the micr...
Transient Cyclic Structured Oligonucleotide Designs for Therapeutic Applications [0.03%]
用于治疗应用的瞬时循环结构化寡核苷酸设计
Sudhir Agrawal
Sudhir Agrawal
Oligonucleotide-based therapeutics are now widely used in clinical settings. From the late 1980s to the mid-1990s, efforts to improve therapeutic efficacy focused on imparting drug-like properties to oligonucleotides, emphasizing nuclease s...
Pia Kahnau
Pia Kahnau
The IntelliCage is a home-cage-based radiofrequency identification (RFID) test system for studying learning behavior of group-housed mice. Specifically, the mice must learn where and how to get water within the IntelliCage. The protocol des...
Imaging Flow Cytometric Assessment of del(17p) and amp(1q21) in Circulating Plasma Cells of Myeloma Patients [0.03%]
影像流式细胞仪检测多发性骨髓瘤患者循环浆细胞中del(17p)和amp(1q21)的状态
Thomas I Mincherton,Matthew Harms,Sarah E Clarke et al.
Thomas I Mincherton et al.
Multiple myeloma is a bone marrow-derived neoplastic proliferation of plasma cells with low levels of circulating tumor cells. Cytogenetic abnormalities are present in >90% of patients, when assessed by fluorescence in situ hybridization (F...
From Struggling (With) Screening Tests to Mouse Models of Depression-Relevant Neurobehavioral States [0.03%]
从抑郁症行为神经模型的筛选到抑郁相关的神经行为状态的小鼠模型
Christopher R Pryce
Christopher R Pryce
A major, serendipitous psychiatric discovery is monoamine-transporter reuptake inhibition as an antidepressant mechanism of action. Chronic treatment with such antidepressants is efficacious, with onset requiring 1-2 weeks, in many but by n...
Rapid, Scalable, and Cost-Effective Manufacturing of Uniform Non-Enveloped, Tag-Free Virus-Like Particles [0.03%]
快速、可扩展且低成本的制造均一非包膜无标签病毒样颗粒的方法
Xiaoyan Wang,Yanhao Gu,Hong Luo et al.
Xiaoyan Wang et al.
Virus-like particles (VLPs) are widely recognized as safe and versatile nanostructures with broad applications in vaccine development, gene delivery, and nanotechnology, but their production typically requires time-consuming procedures, inc...
A Beginner's Guide to Using DeepVirFinder for Viral Sequence Identification From Metagenomic Datasets [0.03%]
DeepVirFinder病毒序列识别入门指南：基于元基因组数据集
Yuqian Mo,Nathan Ahlgren,Jed A Fuhrman et al.
Yuqian Mo et al.
Identifying viral sequences from metagenomic datasets is critical for investigating their origins, evolutionary patterns, and ecological functions. Previously, we developed a novel deep learning software, DeepVirFinder, to predict viral seq...