Co-Culture Systems to Study Epithelial-Immune Interactions During SARS-CoV-2 Infection [0.03%]
用于研究SARS-CoV-2感染期间上皮细胞-免疫细胞相互作用的共培养系统
Scott H Randell,Katherine C Barnett
Scott H Randell
Robust inflammatory responses to viral infection are mediated by immune cell populations, including monocytes and dendritic cells. However, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) does not replicate efficiently in these...
Acid-Catalyzed Cycloaddition of Guanines with TMOP: A Unified Strategy for Synthesizing Tricyclic Acyclovir Analogues and M1G-Containing Oligonucleotides [0.03%]
酸催化鸟嘌呤与TMOP的环加成反应：合成三环阿昔洛韦类似物和含M1G的寡核苷酸的统一策略
Zhuo-Yue Jia,Bin Wu,Wen-Wu Sun
Zhuo-Yue Jia
This article describes an efficient acid-catalyzed cyclization strategy for constructing the tricyclic pyrimido[1,2-a]purin-10(3H)-one scaffold via the reaction of guanine derivatives with 1,1,3,3-tetramethoxypropane (TMOP) catalyzed by 2,4...
A Hierarchical Screening Strategy for Genome-Edited Events in Polyploid Species: A Case Study on Hexaploid Common Wheat [0.03%]
六倍体普通小麦基因组编辑事件的层次筛选策略研究
Mingjiu Li,Guofeng Gao,Yuling Jiao
Mingjiu Li
Genome editing via CRISPR/Cas9 has been widely adopted in cereal crops. In diploid species such as rice and barley, the generation of knockout mutants is relatively straightforward for functional characterization of the genes of interest du...
A Tutorial for Isolating, Characterizing, and Inducing Presenescence in Human Periodontal Ligament and Dental Pulp Stem Cells [0.03%]
牙周膜和牙髓干细胞衰老诱导、鉴定及生物学特性分析的实验方法学研究
Kamila Sauer Veiga Leme,Márjorie de Assis Golim,Aline Márcia Marques Braz et al.
Kamila Sauer Veiga Leme et al.
For several years, scientists have focused on studying mesenchymal stem cells because of their ability for self-regeneration, their potential for differentiation into multiple lineages (e.g., osteogenic, chondrogenic, and adipogenic cells),...
Synthesis of 2',4'- and 3',4'-Bridged Nucleosides via 4'-Carbon Radicals Generated by H-Abstraction [0.03%]
通过H- abstraction生成的4'-碳自由基合成2',4'-和3',4'-桥连核苷
Yuta Ito,Yoshiyuki Hari
Yuta Ito
This unit describes a method for the synthesis of 2',4'- and 3',4'-bridged nucleosides from nucleosides bearing oxime imidate moieties at the 5' position. Hydrogen abstraction at the 4'-position of nucleosides by iminyl radicals derived fro...
Synthesis of Oligonucleotide RNA Containing 2´-Deoxy-2´-Fluoroarabinoguanosine (2´F-araG) for Stabilization of the Human Telomeric RNA G-Quadruplex [0.03%]
合成含有2'脱氧-2'-氟阿拉伯果糖腺苷(2'F-araG)的人类端粒RNA-G四联体稳定的寡核苷酸RNA
Hisao Saneyoshi,Rie Iwakiri,Shiyu Wang et al.
Hisao Saneyoshi et al.
This article provides detailed synthetic protocols for the preparation of RNA oligonucleotides containing 2'-deoxy-2'-fluoroarabinoguanosine (2'F-araG). Incorporation of this modification into a human telomeric RNA sequence stabilizes the G...
Correction: An Optimized Protocol for the Generation of iPSC Lines from Lymphoblastoid Cell Lines Using the Non-integrative Sendai Virus [0.03%]
纠正：使用非整合沙甸病毒生成诱导多能干细胞系的淋巴母细胞系的优化方案
Laurie Martineau,Isabella Bienjonetti,Sabrina Bellenfant et al.
Laurie Martineau et al.
Published Erratum
Current protocols. 2026 Mar;6(3):e70367. DOI:10.1002/cpz1.70367 2026
Optimized High-Titer Lentivirus Production and Efficient CAR-T Cell Generation [0.03%]
高滴度慢病毒生产及高效CAR-T细胞制备的优化
Atul Kumar Roy,Ashwani Singh,Jyotsna Dandotiya et al.
Atul Kumar Roy et al.
Chimeric antigen receptor (CAR)-T cell treatment has shifted the paradigm of cancer immunotherapy, especially in B-cell malignancies, with around an 80% response rate in acute lymphoblastic leukemia. Emerging data on the use of CAR-T cells ...
Best Practices in Structural Ensemble Analysis: Avoiding Pitfalls, Interpreting Results, and Automating Workflows with EnsembleFlex [0.03%]
结构集合分析的最佳实践：避免陷阱、解释结果和使用EnsembleFlex自动化工作流程
Melanie Schneider,José Antonio Marquez,Andrew R Leach
Melanie Schneider
Structural ensemble analysis is critical for understanding protein flexibility, function, and interactions. EnsembleFlex provides a streamlined framework for dual-scale (backbone-only and all-atom) flexibility analysis by integrating both d...
Analysis of Reactive Oxygen Species-Induced Cellular Damage in Cervical Cancer [0.03%]
宫颈癌中活性氧诱导的细胞损伤分析
Hoi Ching Cherry Chan,Sachin Bhoora,Esther Zhou et al.
Hoi Ching Cherry Chan et al.
Reactive oxygen species (ROS) are highly reactive oxygen-based molecules comprising hydrogen peroxide, hydroxyl radicals, superoxide anion, and singlet oxygen. These species are produced intracellularly and play an important role in cellula...