A Standardized Unpredictable Chronic Stress Protocol for Adult Zebrafish [0.03%]
适用于成年斑马鱼的标准化不可预测慢性压力方案
Matheus Gallas-Lopes,Ana P Herrmann,Angelo Piato
Matheus Gallas-Lopes
The unpredictable chronic stress (UCS) protocol, originating in the 1980s, has contributed to depression research by inducing behavioral and physiological changes in rodents that resemble the symptoms observed in patients. Its translational...
Cholesterol-Induced Senescent Macrophages and Evaluation of Cellular Senescence Phenotypes [0.03%]
胆固醇诱导的衰老巨噬细胞及细胞衰老表型评估
Ivan A Salladay-Perez,Anthony J Covarrubias
Ivan A Salladay-Perez
Recent discoveries linking cellular senescence to inflammaging and age-associated metabolic diseases have intensified interest in defining the mechanisms that drive cellular senescence. Single-cell transcriptomic studies have identified tis...
Determination of Total Soluble Sugars in Pteridophytes Using the Anthrone Method [0.03%]
蒽酮法测定pteridophyta中的总可溶性糖类
Pablo Denova-Lozano,Alejandra Chamorro-Flores,Amparo B Cerón-Carpio et al.
Pablo Denova-Lozano et al.
Sugars serve as crucial integrators of both internal and environmental signals in plants, shaping the regulation of diverse physiological processes that occur throughout the plant's life span, from early embryogenesis to later senescence st...
Michelle C Krzyzanowski,Helen Pan,Iris Glaze et al.
Michelle C Krzyzanowski et al.
Because of the urgent need to better understand the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission mechanism and clinical outcomes resulting from infection, health organizations and research institutes developed d...
Grasiela Torres,Utkarsh Tripathi,Ivan Salladay-Perez et al.
Grasiela Torres et al.
We present a reproducible in vitro protocol for harvesting and culturing murine bone marrow-derived macrophages, with the added capability of freezing bone marrow cells at -80°C for scalability and long-term storage. To induce macrophage s...
Laboratory Protocols for Candidatus Liberibacter asiaticus Transmission Biology and Management Research [0.03%]
亚洲 Liberibacter asiaticus 传输生物学和管理研究的实验室方案
Diogo M Galdeano,William Ingram,Anna Erickson et al.
Diogo M Galdeano et al.
Candidatus Liberibacter asiaticus (CLas), the causal agent of Huanglongbing, is transmitted by the Asian citrus psyllid (Diaphorina citri) in a persistent, propagative manner. Reproducible transmission studies require not only controlled in...
Multiparametric Characterization of Telomere Length in T Cells by Flow-FISH [0.03%]
流式细胞仪结合荧光原位杂交技术多参数分析T细胞端粒长度
Macedonia Trigueros,Yacine Kharraz,Hammad Alzayat et al.
Macedonia Trigueros et al.
Telomere shortening is a hallmark of aging and immune dysfunction, offering a reliable indicator of cellular replicative history and biological age. Measuring telomere length in defined immune subsets provides critical insights into immune ...
Current Overview and Main Challenges of the Use of Galleria mellonella in Biomedical Research [0.03%]
"Galleria mellonella在生物医学研究中的应用现状及主要挑战"
Fabiana Alves de Souza Silva,Maria Eduarda da Silva Costa,Patrícia Michelle Nagai de Lima et al.
Fabiana Alves de Souza Silva et al.
Larvae of the greater wax moth Galleria mellonella have become an increasingly important in vivo model for biomedical research, providing a practical, ethical, and biologically relevant alternative to vertebrate organisms. Its suitability a...
Cellular Characterization Using Microwave-Assisted Immunocytochemistry, A Time-Efficient Alternative to Traditional Immunocytochemistry [0.03%]
基于微波免疫细胞化学的高效细胞表征新技术
Aidyn M Medina-López,Irma I Torres-Vázquez,Noraida Martínez-Rivera et al.
Aidyn M Medina-López et al.
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are related but separate techniques used for cell and tissue characterization. ICC involves immunolabeling of cells, and IHC immunolabeling of tissues; thus, the main difference is sa...
Absolute Quantification of microRNA Copies in Exosomes Using Real-Time PCR [0.03%]
使用实时PCR法对外泌体中微小核糖核酸进行绝对定量的方法
Pragati Raghuwanshi,Prasenjit Srivastava,Nishant Singh et al.
Pragati Raghuwanshi et al.
Exosomes are small extracellular vesicles (50 to 150 nm in size) that carry different kind of biomolecules in their lumen and facilitate intercellular communication. Serum contains a large number of exosomes (∼1012/ml of serum) that contai...