Expression and Membrane Targeting of β1-integrin-ECFP in Mammalian Cells [0.03%]
哺乳动物细胞中的β1-整合素-ECFP的表达及其膜定位
Andres Martin Toscani,Federico Coluccio Leskow
Andres Martin Toscani
β1-integrin is a transmembrane glycoprotein that heterodimerizes with multiple α subunits and functions as a key receptor for extracellular matrix proteins. It contains a large extracellular ligand-binding domain, a single-pass transmembr...
A Facile Strategy for 3'-Terminal Functionalization of DNA and RNA via On-Column Conjugation with Integration into Tandem Oligonucleotide Synthesis [0.03%]
一种用于DNA和RNA 3'-末端官能化的简便策略及其在串联寡核苷酸合成中的应用
Jagandeep S Saraya,Derek K O&#x;Flaherty
Jagandeep S Saraya
Amino-functionalized oligonucleotides are valuable tools in therapeutics, biosensing, and nanotechnology. Although 5'-terminal modifications are readily accessible, incorporation at the 3'-terminus remains more challenging and often require...
Evaluation of Antibacterial Effects of Sargassum binderi-based Mouthwash via API 20E Profiling: Study Protocol for a Feasibility Randomized Controlled Trial [0.03%]
基于扁芽丝藻漱口水抗菌效果的API 20E特征研究：可行性随机对照试验方案
Ruslim Andre Kusuma,Nisrina Ekayani Nasrun,Muhammad Ruslin et al.
Ruslim Andre Kusuma et al.
The antibacterial effects of Sargassum binderi (brown macroalgae rich in phlorotannins and fucoidan) have not yet been explored in clinical settings, particularly regarding its use as an oral antimicrobial agent. This study aims to evaluate...
Brain Magnetic Resonance Elastography Experiments With an Electromagnetic Actuator [0.03%]
基于电磁激励的脑弹性图实验研究
Suhao Qiu,Yuan Feng,Guang-Zhong Yang
Suhao Qiu
Magnetic resonance elastography (MRE) enables non-invasive assessment of the mechanical properties of brain tissue, which is crucial for diagnosing neurodegenerative diseases and understanding brain development. This technique relies on ind...
Efficient Adaptation of Structure-Aware Protein Language Models for Diverse Protein Applications [0.03%]
高效适应结构感知的蛋白质语言模型以应用于各种蛋白质相关任务
Duolin Wang,Yichuan Zhang,Yongfang Qin et al.
Duolin Wang et al.
Protein language models (PLMs) trained solely on sequence data have significantly advanced our understanding of protein biology and achieved remarkable performance in protein prediction tasks. However, their lack of three-dimensional (3D) s...
Anzu Minami,Minami Shimizu,Shun Tamaki et al.
Anzu Minami et al.
Genome editing can enhance basic research and enable industrial applications of green algae. Here, we present an affordable, broadly applicable workflow for genome editing in the unicellular green alga Euglena gracilis using Cas9 nucleases....
Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal Lung [0.03%]
成人人类肺泡上皮细胞的永生化以研究远端肺部环境暴露
Evelyn Tran,Laurence St Pierre,Diego Velarde et al.
Evelyn Tran et al.
Alveolar epithelial cell type 1 (AT1) and type 2 (AT2) cells make up the saccular gas exchange units of the lung, called alveoli. Formation of alveoli during lung development accounts for the expansive surface area of the lung, allowing for...
Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand [0.03%]
表面等离子体共振（SPR）工作流程比较分析纳米抗体变异体与模型配基溶菌酶的结合能力
Escarlet Díaz-Galicia,Nicoleta Gutu,Yuli Peng et al.
Escarlet Díaz-Galicia et al.
Developing protein interaction-based technologies such as biosensors requires a clear understanding of receptor-target kinetics. Nanobodies, which are camelid-derived single-domain antibodies, are ideal biosensor receptors due to their high...
Point-of-CARE INFRARED IMAGING for Early Detection and Screening of Oral Cancer [0.03%]
口腔癌早期检测与筛查的临场关怀红外成像技术
Shalini R Gupta,Ravibabu Mulaveesala,Mani Kalaivani
Shalini R Gupta
India accounts for one-third of the global incidence and mortality of oral cancer. The national oral cancer screening program uses Conventional Oral Examination by Primary Healthcare (PHC) workers. This subjective assessment leads to unnece...
Cell Painting PLUS: An Iterative Staining-Elution Protocol for High-Content Phenotypic Screenings [0.03%]
细胞染色PLUS：用于高内涵表型筛选的迭代染色-洗脱协议
Marlene Wedler,Elena von Coburg,Jose M Muino et al.
Marlene Wedler et al.
Cell Painting (CP) methods use a combination of fluorescent dyes to label multiple cellular compartments simultaneously, enabling the comprehensive analysis of phenotypic changes through morphological profiling. Here, we present a detailed ...