Correction: An Optimized Protocol for the Generation of iPSC Lines from Lymphoblastoid Cell Lines Using the Non-integrative Sendai Virus [0.03%]
纠正：使用非整合沙甸病毒生成诱导多能干细胞系的淋巴母细胞系的优化方案
Laurie Martineau,Isabella Bienjonetti,Sabrina Bellenfant et al.
Laurie Martineau et al.
Published Erratum
Current protocols. 2026 Mar;6(3):e70367. DOI:10.1002/cpz1.70367 2026
Optimized High-Titer Lentivirus Production and Efficient CAR-T Cell Generation [0.03%]
高滴度慢病毒生产及高效CAR-T细胞制备的优化
Atul Kumar Roy,Ashwani Singh,Jyotsna Dandotiya et al.
Atul Kumar Roy et al.
Chimeric antigen receptor (CAR)-T cell treatment has shifted the paradigm of cancer immunotherapy, especially in B-cell malignancies, with around an 80% response rate in acute lymphoblastic leukemia. Emerging data on the use of CAR-T cells ...
Best Practices in Structural Ensemble Analysis: Avoiding Pitfalls, Interpreting Results, and Automating Workflows with EnsembleFlex [0.03%]
结构集合分析的最佳实践：避免陷阱、解释结果和使用EnsembleFlex自动化工作流程
Melanie Schneider,José Antonio Marquez,Andrew R Leach
Melanie Schneider
Structural ensemble analysis is critical for understanding protein flexibility, function, and interactions. EnsembleFlex provides a streamlined framework for dual-scale (backbone-only and all-atom) flexibility analysis by integrating both d...
Analysis of Reactive Oxygen Species-Induced Cellular Damage in Cervical Cancer [0.03%]
宫颈癌中活性氧诱导的细胞损伤分析
Hoi Ching Cherry Chan,Sachin Bhoora,Esther Zhou et al.
Hoi Ching Cherry Chan et al.
Reactive oxygen species (ROS) are highly reactive oxygen-based molecules comprising hydrogen peroxide, hydroxyl radicals, superoxide anion, and singlet oxygen. These species are produced intracellularly and play an important role in cellula...
An Open-Source Workflow for Semi-Automated Spatial Profiling of Multiplex Immunofluorescent Images [0.03%]
一种开源工作流程：对多重免疫荧光图像进行半自动空间分析
Alisha Dabb,Cynthia Morgan,Sophia Noble et al.
Alisha Dabb et al.
Multiplex immunofluorescence microscopy facilitates the quantification and spatial analysis of cellular features within tissue sections, allowing greater understanding of disease progression or the effects of drug treatment or other exposur...
How to Apply Social Network Analysis to Evaluate Professional Development Programs in Biomedical Research [0.03%]
如何运用社会网络分析评估生物医学研究中的职业发展项目
Diane B Smith,Carolyn J Hovde,Julia Thom Oxford
Diane B Smith
Effective evaluation and assessment of research-focused programs, including centers, core facilities, and institutes, is crucial but inherently challenging. Faculty members are often departmentalized, yet their research necessitates campus-...
Antisense Oligonucleotide Pulldown and Silencing of Circular RNA Nfix In Vivo in Neonatal Mouse Lungs [0.03%]
circNfix的反义寡核苷酸沉淀及其在新生小鼠肺部的体内干扰作用
Pragnya Das,Sharmistha Shyamal,Vineet Bhandari et al.
Pragnya Das et al.
RNA-based therapeutics are emerging as a powerful platform for disease treatment, and one of the novel RNA molecules with therapeutic potential is circular RNA (circRNA). The ubiquitously expressed circRNAs are covalently closed, single-str...
A Semi-Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels [0.03%]
一种半定量酵母互补平台 用于表征膜通道的尿素和氨转运能力
Anna Stoib,Sahar Shojaei,Christine Siligan et al.
Anna Stoib et al.
Yeast complementation assays provide a robust in vivo platform for characterizing the permeability and pH gating of transmembrane channels. This article details a liquid culture approach to quantify urea and ammonia transport using Saccharo...
An Optimized Protocol for the Generation of iPSC Lines from Lymphoblastoid Cell Lines Using the Non-integrative Sendai Virus [0.03%]
一种使用非整合性赛代氏病毒从淋巴母细胞系中生成iPSC系的优化方案
Laurie Martineau,Isabella Bienjonetti,Sabrina Bellenfant et al.
Laurie Martineau et al.
The choice of cell source for generating induced pluripotent stem cells (iPSCs) is influenced by several factors, including accessibility, reprogramming efficiency, and the invasiveness of the cell collection procedure. Traditional sources ...
Metagenomic Hi‑C Protocols for Viral Genome Binning, Taxonomic Annotation, and Interaction Network Visualization [0.03%]
元基因组Hi-C文库构建、病毒分类和互作网络解析技术及应用
Yuxuan Du,Yuqiu Wang,Fengzhu Sun
Yuxuan Du
Metagenomic Hi-C (metaHi-C) links mobile genetic elements to their cellular hosts directly within complex microbial communities. Once shotgun and Hi-C libraries have been generated, however, the main challenges shift to the bioinformatics r...